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Article

Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy

  • Authors:
    • Jibin Sha
    • Bo Sui
    • Xiaoqing Su
    • Qingfang Meng
    • Chenggang Zhang
  • View Affiliations / Copyright

    Affiliations: School of Sports Science and Health, Shandong Sports University, Jinan, Shandong 250102, P.R. China, Department of Endoscopy, Zhangqiu People's Hospital, Jinan, Shandong 250200, P.R. China, School of Social Sports Science, Shandong Sports University, Jinan, Shandong 250102, P.R. China, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China
  • Pages: 7715-7723
    |
    Published online on: September 19, 2017
       https://doi.org/10.3892/mmr.2017.7522
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Abstract

Diabetic nephropathy (DN) is one of the most significant long‑term complications in terms of morbidity and mortality for diabetic patients; however, the exact cause remains unknown. To address this, the DN model was established, and oxidative stress indexes, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px), and inflammatory cytokines, includinginterleukin‑6 (IL‑6), tumor necrosis factor‑alpha (TNF‑α) and transforming growth factor‑beta (TGF‑β), were examined by ELISA. Renal pathological alterations and cell apoptosis was examined by hematoxylin and eosin and terminal deoxynucleotidyl transferase mediated dUTP nick‑end labeling staining, respectively. The expression levels of B‑cell lymphoma‑2 (Bcl‑2), Bcl‑2 associated X (Bax) and caspase‑3 wereexamined by immunohistochemistry and western blotting. The DN model was correctly established, with lower body weight and the higher blood glucose in the diabetes model group. The expression levels of SOD and GSH‑Px were significantly decreased in the diabetes model group; however, MDA, IL‑6, TNF‑α and TGF‑β were significantly increased. The kidney was severely damaged in the diabetes model group, with inflammatory cell invasion, increasing amount of interstitial matrix and hypertrophy with vacuolar degeneration of renal tubular cells. Cell apoptosis levels were significantly increased, and Bcl‑2 was significantly decreased in the diabetes model group in contrast with that of the sham group; however, Bax and caspase‑3 were significantly increased. It suggested that increased oxidative stress and inflammatory cytokines may enhance the apoptosis levels in DN, and may provide a significant diagnostic reference for DN in diabetes patients.
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Copy and paste a formatted citation
Spandidos Publications style
Sha J, Sui B, Su X, Meng Q and Zhang C: Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy. Mol Med Rep 16: 7715-7723, 2017.
APA
Sha, J., Sui, B., Su, X., Meng, Q., & Zhang, C. (2017). Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy. Molecular Medicine Reports, 16, 7715-7723. https://doi.org/10.3892/mmr.2017.7522
MLA
Sha, J., Sui, B., Su, X., Meng, Q., Zhang, C."Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy". Molecular Medicine Reports 16.5 (2017): 7715-7723.
Chicago
Sha, J., Sui, B., Su, X., Meng, Q., Zhang, C."Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy". Molecular Medicine Reports 16, no. 5 (2017): 7715-7723. https://doi.org/10.3892/mmr.2017.7522
Copy and paste a formatted citation
x
Spandidos Publications style
Sha J, Sui B, Su X, Meng Q and Zhang C: Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy. Mol Med Rep 16: 7715-7723, 2017.
APA
Sha, J., Sui, B., Su, X., Meng, Q., & Zhang, C. (2017). Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy. Molecular Medicine Reports, 16, 7715-7723. https://doi.org/10.3892/mmr.2017.7522
MLA
Sha, J., Sui, B., Su, X., Meng, Q., Zhang, C."Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy". Molecular Medicine Reports 16.5 (2017): 7715-7723.
Chicago
Sha, J., Sui, B., Su, X., Meng, Q., Zhang, C."Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy". Molecular Medicine Reports 16, no. 5 (2017): 7715-7723. https://doi.org/10.3892/mmr.2017.7522
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