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Article

Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice

  • Authors:
    • Chenxiao Li
    • Jihan Xia
    • Wenjuan Zhu
    • Leilei Xin
    • Cuiping An
    • Shulin Yang
    • Kui Li
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China
  • Pages: 7738-7744
    |
    Published online on: September 20, 2017
       https://doi.org/10.3892/mmr.2017.7530
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Abstract

Glucocorticoids are associated with lipid metabolism and their abnormal expression has an important function in the development of metabolic syndrome. The 11β‑hydroxysteroid dehydrogenase type 1 (11β‑HSD1) is a metabolic enzyme of glucocorticoids and may be a potential drug target for the treatment of metabolic syndrome. However, the association between the systemic expression of 11β‑HSD1 and metabolic syndrome remains to be elucidated. The present study used a cytomegalovirus promoter to obtain mice that systemically overexpressed the 11β‑HSD1 gene. The transgenic mice and negative control groups received a high‑fat diet at the age of 10 weeks in order to induce metabolic syndrome and this diet was continued for 12 weeks. Several indicators, including body weight, blood glucose, glucose tolerance and insulin resistance, were monitored in vivo. In addition, the protein expression levels of 11β‑HSD1 and DNA damage inducible transcript 3 were detected and the histopathology of important tissues for metabolic syndrome were analyzed. The current findings revealed that the body weights of transgenic mice were significantly higher compared with the control group before and during the periods of high fat diet induction. Transgenic mice also exhibited significantly impaired glucose tolerance, insulin resistance, endoplasmic reticulum stress and increased metabolic syndrome‑associated biochemical indicators in the blood and severely impaired liver and kidney functions. The present study successfully established a 11β‑HSD1 systemic overexpression mouse model that exhibited typical characteristics of metabolic syndrome and may be useful for future studies of metabolic syndrome.
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Copy and paste a formatted citation
Spandidos Publications style
Li C, Xia J, Zhu W, Xin L, An C, Yang S and Li K: Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice. Mol Med Rep 16: 7738-7744, 2017.
APA
Li, C., Xia, J., Zhu, W., Xin, L., An, C., Yang, S., & Li, K. (2017). Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice. Molecular Medicine Reports, 16, 7738-7744. https://doi.org/10.3892/mmr.2017.7530
MLA
Li, C., Xia, J., Zhu, W., Xin, L., An, C., Yang, S., Li, K."Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice". Molecular Medicine Reports 16.5 (2017): 7738-7744.
Chicago
Li, C., Xia, J., Zhu, W., Xin, L., An, C., Yang, S., Li, K."Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice". Molecular Medicine Reports 16, no. 5 (2017): 7738-7744. https://doi.org/10.3892/mmr.2017.7530
Copy and paste a formatted citation
x
Spandidos Publications style
Li C, Xia J, Zhu W, Xin L, An C, Yang S and Li K: Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice. Mol Med Rep 16: 7738-7744, 2017.
APA
Li, C., Xia, J., Zhu, W., Xin, L., An, C., Yang, S., & Li, K. (2017). Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice. Molecular Medicine Reports, 16, 7738-7744. https://doi.org/10.3892/mmr.2017.7530
MLA
Li, C., Xia, J., Zhu, W., Xin, L., An, C., Yang, S., Li, K."Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice". Molecular Medicine Reports 16.5 (2017): 7738-7744.
Chicago
Li, C., Xia, J., Zhu, W., Xin, L., An, C., Yang, S., Li, K."Systemic overexpression of the 11β‑HSD1 promotes endoplasmic reticulum stress in multiple tissues and the development of metabolic syndrome in mice". Molecular Medicine Reports 16, no. 5 (2017): 7738-7744. https://doi.org/10.3892/mmr.2017.7530
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