Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway

Fan,Lin; Xiong,Yan; Fu,Zhen; Xu,Dingfeng; Wang,Lei; Chen,Yong; Xia,Haoyang; Peng,Na; Ye,Shaojun; Wang,Yanfeng; Zhang,Lina; Ye,Qifa

doi:10.3892/mmr.2017.7534

Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway

Authors:
- Lin Fan
- Yan Xiong
- Zhen Fu
- Dingfeng Xu
- Lei Wang
- Yong Chen
- Haoyang Xia
- Na Peng
- Shaojun Ye
- Yanfeng Wang
- Lina Zhang
- Qifa Ye
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Affiliations: Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan, Hubei 430071, P.R. China, Research Center of National Health Ministry on Transplantation Medicine Engineering and Technology, The 3rd Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, College of Chemistry & Molecule Sciences, Wuhan University, Wuhan, Hubei 430081, P.R. China, Department of Abdominal Laparoscopic Surgery, Qinghai University Affiliated Hospital, Xining, Qinghai 810001, P.R. China
Published online on: September 20, 2017 https://doi.org/10.3892/mmr.2017.7534
Pages: 7534-7540
Copyright: © Fan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A previous study has demonstrated a progression in the nerve regeneration by polyaniline/cellulose (PANI/RC), although the underlying mechanism was not elucidated. In the present study, regenerated nerves were investigated, using histological techniques, functional assays and western blot analysis. The triceps surae muscle weight ratio percentages of the sham, regenerated cellulose (RC) and the PANI/RC groups were 38.88±4.76 and 76.32±7.11%, respectively. The thickness of the myelin sheath for the aforementioned groups were as follows: 1.2±0.27; 0.49±0.21 and 0.93±0.28 µl. Western blot analysis demonstrated that the ciliary neurotrophic factor (CNTF) and brain‑derived neurotrophic factor (BDNF) were highly expressed in the regenerated nerve in the presence of polyaniline. Phosphorylated extracellular kinase (p‑ERK)1/2 expression in the PANI/RC group was significantly elevated compared with the RC group (1.83‑fold) and the sham group (4.92‑fold). The expression of the axon sprout‑associated proteins, such as Tau, α‑tubulin and growth associated protein‑43, were increased (1.64, 1.59 and 1.24‑fold, respectively) compared with the RC group. The results demonstrated that PANI enhances the expression and secretion of BDNF and CNTF, activates the ERK1/2 signaling pathway and increases the expression levels of the GAP‑43, Tau and α‑tubulin, suggesting an insight into nerve regeneration and possible clinical interventions in nerve injury.

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