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Article

Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway

  • Authors:
    • Yu Shunzhi
    • Li Zhonghai
    • Yan Ning
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, Department of Orthopedics, First Affiliated Hospital of Dalian Medical University, Dalian 116011, P.R. China
  • Pages: 7692-7698
    |
    Published online on: September 20, 2017
       https://doi.org/10.3892/mmr.2017.7543
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Abstract

The aim of the present study was to investigate the effects of mechanical stress on the osteogenic differentiation of human ligamentum flavum cells via the bone morphogenetic protein (BMP)‑Smad1 signaling pathway. Mechanical stress increased cell proliferation and induced osteogenic differentiation of human cells derived from the ossification of the ligamentum flavum (OLF). In addition, mechanical stress activated osteocalcin (OC), alkaline phosphatase (ALP) and runt‑related transcription factor 2 (RUNX‑2) mRNA expression, and suppressed Ets proto‑oncogene 1 (Ets‑1) and sex determining region Y‑box 2 (SOX‑2) mRNA expression in OLF cells. Src protein expression was suppressed by mechanical stress in human OLF cells. In addition, the protein expression levels of BMP, phosphorylated (p)‑mothers against decapentaplegic homolog‑1 (Smad1) and p‑p38‑mitogen‑activated protein kinases (p38MAPK) were increased by mechanical stress. These results demonstrate that mechanical stress effectively increases cell proliferation, promotes the osteogenic differentiation rate of OLF cells, activates OC, ALP and RUNX‑2, and suppresses Ets‑1 and SOX‑2 potentially via the BMP‑Smad1 and Src‑p38MAPK signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Shunzhi Y, Zhonghai L and Ning Y: Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway. Mol Med Rep 16: 7692-7698, 2017.
APA
Shunzhi, Y., Zhonghai, L., & Ning, Y. (2017). Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway. Molecular Medicine Reports, 16, 7692-7698. https://doi.org/10.3892/mmr.2017.7543
MLA
Shunzhi, Y., Zhonghai, L., Ning, Y."Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway". Molecular Medicine Reports 16.5 (2017): 7692-7698.
Chicago
Shunzhi, Y., Zhonghai, L., Ning, Y."Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway". Molecular Medicine Reports 16, no. 5 (2017): 7692-7698. https://doi.org/10.3892/mmr.2017.7543
Copy and paste a formatted citation
x
Spandidos Publications style
Shunzhi Y, Zhonghai L and Ning Y: Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway. Mol Med Rep 16: 7692-7698, 2017.
APA
Shunzhi, Y., Zhonghai, L., & Ning, Y. (2017). Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway. Molecular Medicine Reports, 16, 7692-7698. https://doi.org/10.3892/mmr.2017.7543
MLA
Shunzhi, Y., Zhonghai, L., Ning, Y."Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway". Molecular Medicine Reports 16.5 (2017): 7692-7698.
Chicago
Shunzhi, Y., Zhonghai, L., Ning, Y."Mechanical stress affects the osteogenic differentiation of human ligamentum flavum cells via the BMP‑Smad1 signaling pathway". Molecular Medicine Reports 16, no. 5 (2017): 7692-7698. https://doi.org/10.3892/mmr.2017.7543
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