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Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma

  • Authors:
    • Xinchao Deng
    • Congzhe Hou
    • Huali Wang
    • Tingting Liang
    • Lin Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Second Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7497-7503
    |
    Published online on: September 20, 2017
       https://doi.org/10.3892/mmr.2017.7564
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Abstract

Endometrial carcinoma is the most common malignancy of the female genital tract and is the fourth most common malignancy among women worldwide. Endometrial adenocarcinoma (EAC) accounts for ~80% of endometrial carcinoma cases. Numerous critical genetic events have been determined to serve an essential role in EAC progression; however, the precise molecular mechanisms underlying EAC progression remain unclear. Pyrosequencing and methylation‑specific PCR were used to detect the methylation status of Wnt inhibitory factor 1 (WIF1). Immunohistochemistry and western blot were used to detect the expression of WIF1, Wnt family member 1 and other related pathways. The anticancer role of WIF1 in EAC was investigated in vitro and in vivo. Two of the three EAC cases exhibited significantly high methylation in five CpG sites, and the WIF1 methylation rate in EAC and endometrial tissues was 43.4 and 8%, respectively (P<0.05). The kappa consistency coefficient was ‑0.369 between methylation and mRNA expression (P<0.05) and WIF1 expression levels were significantly downregulated in EAC tissues compared with non‑tumorous tissues (P<0.01). The 5‑year overall survival rates were significantly lower for patients with tumors that negatively expressed WIF1 when compared with the 77.9% exhibited by those with positive WIF1 expression. Furthermore, the proliferation rate of KLE cells was significantly reduced following 5‑aza‑20‑deoxycytidine treatment or WIF1 overexpression in vitro and in vivo, which may be associated with downregulated c‑Myc and phosphorylated‑extracellular signal‑regulated kinase expression. These results demonstrated the important role of WIF1 in EAC tumorigenesis, and suggested that WIF1 may be a potential drug target in EAC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Deng X, Hou C, Wang H, Liang T and Zhu L: Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma. Mol Med Rep 16: 7497-7503, 2017.
APA
Deng, X., Hou, C., Wang, H., Liang, T., & Zhu, L. (2017). Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma. Molecular Medicine Reports, 16, 7497-7503. https://doi.org/10.3892/mmr.2017.7564
MLA
Deng, X., Hou, C., Wang, H., Liang, T., Zhu, L."Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma". Molecular Medicine Reports 16.5 (2017): 7497-7503.
Chicago
Deng, X., Hou, C., Wang, H., Liang, T., Zhu, L."Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma". Molecular Medicine Reports 16, no. 5 (2017): 7497-7503. https://doi.org/10.3892/mmr.2017.7564
Copy and paste a formatted citation
x
Spandidos Publications style
Deng X, Hou C, Wang H, Liang T and Zhu L: Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma. Mol Med Rep 16: 7497-7503, 2017.
APA
Deng, X., Hou, C., Wang, H., Liang, T., & Zhu, L. (2017). Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma. Molecular Medicine Reports, 16, 7497-7503. https://doi.org/10.3892/mmr.2017.7564
MLA
Deng, X., Hou, C., Wang, H., Liang, T., Zhu, L."Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma". Molecular Medicine Reports 16.5 (2017): 7497-7503.
Chicago
Deng, X., Hou, C., Wang, H., Liang, T., Zhu, L."Hypermethylation of WIF1 and its inhibitory role in the tumor growth of endometrial adenocarcinoma". Molecular Medicine Reports 16, no. 5 (2017): 7497-7503. https://doi.org/10.3892/mmr.2017.7564
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