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Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells

  • Authors:
    • Wei Ji
    • Shijia Liu
    • Xia Zhao
    • Yunke Guo
    • Sha Xia
    • Yueyang Lu
    • Menyun Yin
    • Xiao Xu
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatology and Immunology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China, Laboratory of Clinical Pharmacology, Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China, Hematology‑Oncology Department, Zhenjiang Hospital of Integrated Chinese and Western Medicine, Zhenjiang, Jiangsu 212000, P.R. China, College of Basic Medical Sciences, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China, College of Nursing, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China
    Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7391-7397
    |
    Published online on: September 21, 2017
       https://doi.org/10.3892/mmr.2017.7568
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Abstract

Ankylosing spondylitis (AS) is characterized by the formation of bony spurs. Treatment of the resulting ankylosis, excessive bone formation and associated functional impairment, remain the primary therapeutic aims in research regarding this condition. Triptolide is the primary active component of the perennial vine Tripterygium wilfordii Hook. f., and has previously been demonstrated to exert anti‑tumor activities including inhibition of cell growth and the induction of apoptosis, however, the effect of triptolide on osteoblasts remains to be elucidated. In the present study, the MC3T3‑E1 mouse osteoblast cell line was treated with differing concentrations of triptolide for various intervals. Cell proliferation was detected using the bromodeoxyuridine assay, cell cycle and apoptosis were measured by flow cytometry, nuclear apoptosis was observed by Hoechst staining and associated proteins were determined via western blot analysis. The cells were then further incubated with osteogenic induction medium supplemented with triptolide for 7 or 12 days and the differentiation to osteoblasts was examined by picrosirius staining, observation of alkaline phosphatase activity and a calcium deposition assay. It was demonstrated that treatment with triptolide significantly inhibited osteoblast proliferation and induced cell cycle arrest and apoptosis of the osteoblasts. Furthermore, treatment with triptolide reduced collagen formation, alkaline phosphatase activity and calcium deposition. The present study demonstrated an inhibitory effect of triptolide on osteoblast proliferation and differentiation, and therefore suggests a potential therapeutic agent for the treatment of AS in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Ji W, Liu S, Zhao X, Guo Y, Xia S, Lu Y, Yin M and Xu X: Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells. Mol Med Rep 16: 7391-7397, 2017.
APA
Ji, W., Liu, S., Zhao, X., Guo, Y., Xia, S., Lu, Y. ... Xu, X. (2017). Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells. Molecular Medicine Reports, 16, 7391-7397. https://doi.org/10.3892/mmr.2017.7568
MLA
Ji, W., Liu, S., Zhao, X., Guo, Y., Xia, S., Lu, Y., Yin, M., Xu, X."Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16.5 (2017): 7391-7397.
Chicago
Ji, W., Liu, S., Zhao, X., Guo, Y., Xia, S., Lu, Y., Yin, M., Xu, X."Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16, no. 5 (2017): 7391-7397. https://doi.org/10.3892/mmr.2017.7568
Copy and paste a formatted citation
x
Spandidos Publications style
Ji W, Liu S, Zhao X, Guo Y, Xia S, Lu Y, Yin M and Xu X: Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells. Mol Med Rep 16: 7391-7397, 2017.
APA
Ji, W., Liu, S., Zhao, X., Guo, Y., Xia, S., Lu, Y. ... Xu, X. (2017). Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells. Molecular Medicine Reports, 16, 7391-7397. https://doi.org/10.3892/mmr.2017.7568
MLA
Ji, W., Liu, S., Zhao, X., Guo, Y., Xia, S., Lu, Y., Yin, M., Xu, X."Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16.5 (2017): 7391-7397.
Chicago
Ji, W., Liu, S., Zhao, X., Guo, Y., Xia, S., Lu, Y., Yin, M., Xu, X."Triptolide inhibits proliferation, differentiation and induces apoptosis of osteoblastic MC3T3‑E1 cells". Molecular Medicine Reports 16, no. 5 (2017): 7391-7397. https://doi.org/10.3892/mmr.2017.7568
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