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Article Open Access

Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells

  • Authors:
    • Silong Lou
    • Jia Ji
    • Xin Cheng
    • Jian Ruan
    • Rong Li
    • Zhengjun Guo
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Chongqing Cancer Hospital, Chongqing 400030, P.R. China, Institute of Pathology and Southwest Cancer Center, and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Third Military Medical University, Chongqing 400038, P.R. China
    Copyright: © Lou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7221-7228
    |
    Published online on: September 21, 2017
       https://doi.org/10.3892/mmr.2017.7572
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Abstract

Glioblastoma multiforme (GBM) is the leading type of brain tumor, exhibiting unlimited proliferation and invasion potential. The present study indicated that a high expression level of miR‑132 was detected in the neural subtype of GBM and predicted an unfavorable prognosis for patients from The Cancer Genome Atlas cohort (n=526). Cox hazard regression analysis demonstrated miR‑132 as an independent prognostic indicator for GBM patients. Further in vitro experiments indicated that miR‑132 promoted the proliferation and sphere formation of U87 cells. Unsupervised hierarchical clustering analysis was performed to compare differently expressed genes between two Gene Expression Omnibus (GEO) datasets and Gene Ontology analysis was applied to evaluate the significant signaling pathways modulated by miR‑132 in GBM cells within a genetic bioinformatic lab, the Gene‑Cloud of Biotechnology Information. By combining the results based on GEO datasets and the miRNA bioinformatic prediction, polypyrimidine tract‑binding protein 2 (PTBP2), a brain tissue‑specific post‑transcriptional protein, was identified as a potential downstream target of miR‑132 in GBM. Thus, miR‑132 overexpression in GBM cells predicted an unfavorable outcome for patients, and sustained the proliferation and self‑renewal abilities of GBM cells in an miR‑132/PTBP2 signaling pathway.
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1 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Miller KD, Siegel RL, Lin CC, Mariotto AB, Kramer JL, Rowland JH, Stein KD, Alteri R and Jemal A: Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin. 66:271–289. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Berindan-Neagoe I, Pdel C Monroig, Pasculli B and Calin GA: MicroRNAome genome: A treasure for cancer diagnosis and therapy. CA Cancer J Clin. 64:311–336. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Chen CZ: MicroRNAs as oncogenes and tumor suppressors. N Engl J Med. 353:1768–1771. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Lei CJ, Li L, Gao X, Zhang J, Pan QY, Long HC, Chen CZ, Ren DF and Zheng G: hsa-miR-132 inhibits proliferation of hepatic carcinoma cells by targeting YAP. Cell Biochem Funct. 33:326–333. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Li W, Zhang J, Chen T, Yin P, Yang J and Cao Y: miR-132 upregulation promotes gastric cancer cell growth through suppression of FoxO1 translation. Tumour Biol. Aug 23–2015.(Epub ahead of print).

8 

Mokutani Y, Uemura M, Munakata K, Okuzaki D, Haraguchi N, Takahashi H, Nishimura J, Hata T, Murata K, Takemasa I, et al: Down-Regulation of microRNA-132 is associated with poor prognosis of colorectal cancer. Ann Surg Oncol. 23 Suppl 5:S599–S608. 2016. View Article : Google Scholar

9 

Tavolaro S, Colombo T, Chiaretti S, Peragine N, Fulci V, Ricciardi MR, Messina M, Bonina S, Brugnoletti F, Marinelli M, et al: Increased chronic lymphocytic leukemia proliferation upon IgM stimulation is sustained by the upregulation of miR-132 and miR-212. Genes Chromosomes Cancer. 54:222–234. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Makeyev EV, Zhang J, Carrasco MA and Maniatis T: The MicroRNA miR-124 promotes neuronal differentiation by triggering brain-specific alternative pre-mRNA splicing. Mol Cell. 27:435–448. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Magill ST, Cambronne XA, Luikart BW, Lioy DT, Leighton BH, Westbrook GL, Mandel G, Goodman RH, et al: microRNA-132 regulates dendritic growth and arborization of newborn neurons in the adult hippocampus. Proc Natl Acad Sci USA. 107:20382–20387. 2010; View Article : Google Scholar : PubMed/NCBI

12 

Kawashima H, Numakawa T, Kumamaru E, Adachi N, Mizuno H, Ninomiya M, Kunugi H and Hashido K: Glucocorticoid attenuates brain-derived neurotrophic factor-dependent upregulation of glutamate receptors via the suppression of microRNA-132 expression. Neuroscience. 165:1301–1311. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Shaked I, Meerson A, Wolf Y, Avni R, Greenberg D, Gilboa-Geffen A and Soreq H: MicroRNA-132 potentiates cholinergic anti-inflammatory signaling by targeting acetylcholinesterase. Immunity. 31:965–973. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Miller BH, Zeier Z, Xi L, Lanz TA, Deng S, Strathmann J, Willoughby D, Kenny PJ, Elsworth JD, Lawrence MS, et al: MicroRNA-132 dysregulation in schizophrenia has implications for both neurodevelopment and adult brain function. Proc Natl Acad Sci USA. 109:3125–3130. 2012; View Article : Google Scholar : PubMed/NCBI

15 

Lee ST, Chu K, Im WS, Yoon HJ, Im JY, Park JE, Park KH, Jung KH, Lee SK, Kim M and Roh JK: Altered microRNA regulation in Huntington's disease models. Exp Neurol. 227:172–179. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Liu Q, Liao F, Wu H, Cai T, Yang L, Wang ZF and Zou R: Upregulation of miR-132 expression in glioma and its clinical significance. Tumour Biol. 35:12299–12304. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Ruan J, Lou S, Dai Q, Mao D, Ji J and Sun X: Tumor suppressor miR-181c attenuates proliferation, invasion, and self-renewal abilities in glioblastoma. Neuroreport. 26:66–73. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Setty M, Helmy K, Khan AA, Silber J, Arvey A, Neezen F, Agius P, Huse JT, Holland EC and Leslie CS: Inferring transcriptional and microRNA-mediated regulatory programs in glioblastoma. Mol Syst Biol. 8:6052012. View Article : Google Scholar : PubMed/NCBI

20 

An F, Zhan Q, Xia M, Jiang L, Lu G, Huang M, Guo J and Liu S: From moderately severe to severe hypertriglyceridemia induced acute pancreatitis: Circulating miRNAs play role as potential biomarkers. PLoS One. 9:e1110582014. View Article : Google Scholar : PubMed/NCBI

21 

Dweep H, Sticht C, Pandey P and Gretz N: miRWalk - database: Prediction of possible miRNA binding sites by ‘walking’ the genes of three genomes. J Biomed Inform. 44:839–847. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Verhaak RG, Hoadley KA, Purdom E, Wang V, Qi Y, Wilkerson MD, Miller CR, Ding L, Golub T, Mesirov JP, et al: Integrated genomic analysis identifies clinically relevant subtypes of glioblastoma characterized by abnormalities in PDGFRA, IDH1, EGFR, and NF1. Cancer Cell. 17:98–110. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Wang H, Li XT, Wu C, Wu ZW, Li YY, Yang TQ, Chen GL, Xie XS, Huang YL, Du ZW and Zhou YX: miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro. Onco Targets Ther. 8:3211–3218. 2015.PubMed/NCBI

24 

Yang Z, Chen Y, Fu Y, Yang Y, Zhang Y, Chen Y and Li D: Meta-analysis of differentially expressed genes in osteosarcoma based on gene expression data. BMC Med Genet. 15:802014. View Article : Google Scholar : PubMed/NCBI

25 

Tian H, Hou L, Xiong YM, Huang JX, Zhang WH, Pan YY and Song XR: miR-132 targeting E2F5 suppresses cell proliferation, invasion, migration in ovarian cancer cells. Am J Transl Res. 8:1492–1501. 2016.PubMed/NCBI

26 

Phillips HS, Kharbanda S, Chen R, Forrest WF, Soriano RH, Wu TD, Misra A, Nigro JM, Colman H, Soroceanu L, et al: Molecular subclasses of high-grade glioma predict prognosis, delineate a pattern of disease progression, and resemble stages in neurogenesis. Cancer Cell. 9:157–173. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Zagore LL, Grabinski SE, Sweet TJ, Hannigan MM, Sramkoski RM, Li Q and Licatalosi DD: RNA binding protein Ptbp2 is essential for male germ cell development. Mol Cell Biol. 35:4030–4042. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Fang D, Yang H, Lin J, Teng Y, Jiang Y, Chen J and Li Y: 17β-estradiol regulates cell proliferation, colony formation, migration, invasion and promotes apoptosis by upregulating miR-9 and thus degrades MALAT-1 in osteosarcoma cell MG-63 in an estrogen receptor-independent manner. Biochem Biophys Res Commun. 457:500–506. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Zheng S, Gray EE, Chawla G, Porse BT, O'Dell TJ and Black DL: PSD-95 is post-transcriptionally repressed during early neural development by PTBP1 and PTBP2. Nat Neurosci. 15:381–388. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Cheung HC, Hai T, Zhu W, Baggerly KA, Tsavachidis S, Krahe R and Cote GJ: Splicing factors PTBP1 and PTBP2 promote proliferation and migration of glioma cell lines. Brain. 132:2277–2288. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Han W, Wang L, Yin B and Peng X: Characterization of a novel posttranslational modification in polypyrimidine tract-binding proteins by SUMO1. BMB Rep. 47:233–238. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lou S, Ji J, Cheng X, Ruan J, Li R and Guo Z: Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells. Mol Med Rep 16: 7221-7228, 2017.
APA
Lou, S., Ji, J., Cheng, X., Ruan, J., Li, R., & Guo, Z. (2017). Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells. Molecular Medicine Reports, 16, 7221-7228. https://doi.org/10.3892/mmr.2017.7572
MLA
Lou, S., Ji, J., Cheng, X., Ruan, J., Li, R., Guo, Z."Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells". Molecular Medicine Reports 16.5 (2017): 7221-7228.
Chicago
Lou, S., Ji, J., Cheng, X., Ruan, J., Li, R., Guo, Z."Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells". Molecular Medicine Reports 16, no. 5 (2017): 7221-7228. https://doi.org/10.3892/mmr.2017.7572
Copy and paste a formatted citation
x
Spandidos Publications style
Lou S, Ji J, Cheng X, Ruan J, Li R and Guo Z: Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells. Mol Med Rep 16: 7221-7228, 2017.
APA
Lou, S., Ji, J., Cheng, X., Ruan, J., Li, R., & Guo, Z. (2017). Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells. Molecular Medicine Reports, 16, 7221-7228. https://doi.org/10.3892/mmr.2017.7572
MLA
Lou, S., Ji, J., Cheng, X., Ruan, J., Li, R., Guo, Z."Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells". Molecular Medicine Reports 16.5 (2017): 7221-7228.
Chicago
Lou, S., Ji, J., Cheng, X., Ruan, J., Li, R., Guo, Z."Oncogenic miR‑132 sustains proliferation and self‑renewal potential by inhibition of polypyrimidine tract‑binding protein 2 in glioblastoma cells". Molecular Medicine Reports 16, no. 5 (2017): 7221-7228. https://doi.org/10.3892/mmr.2017.7572
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