Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
November-2017 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2017 Volume 16 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Peptidome analysis of amniotic fluid from pregnancies with preeclampsia

  • Authors:
    • Yating Qian
    • Lei Zhang
    • Can Rui
    • Hongjuan Ding
    • Pengyuan Mao
    • Hongjie Ruan
    • Ruizhe Jia
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China, Department of Human Resources, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210004, P.R. China
    Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 7337-7344
    |
    Published online on: September 21, 2017
       https://doi.org/10.3892/mmr.2017.7582
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Preeclampsia (PE), a life‑threatening, complicated pregnancy‑associated disease, has recently become a research focus in obstetrics. However, the peptidome of the amniotic fluid in PE patients has rarely been investigated. The present study used peptidomic profiling to perform a comparative analysis of human amniotic fluid between normal and PE pregnancies. Centrifugal ultrafiltration and liquid chromatography‑tandem mass spectrometry (LC‑MS/MS) was combined with isotopomeric dimethyl labels to gain a deeper understanding of the role of proteins and the peptidome in the onset of PE. Following ultrafiltration and LC‑MS/MS, 352 peptides were identified. Of these, 23 peptides were observed to be significantly differentially expressed (6 downregulated and 17 upregulated; P<0.05). Using Gene Ontology and Blastp analyses, the functions and biological activities of these 23 peptides were identified and revealed to include autophagy, signal transduction, receptor activity, enzymatic activity and nucleic acid binding. In addition, a bibliographic search revealed that some of the identified peptides, including Titin, are crucial to the pathogenesis underlying PE. The present study identified 23 peptides expressed at significantly different levels in the amniotic fluid of PE and normal pregnancies. A comprehensive peptidome analysis is more efficient than a simple biomarker analysis at revealing deficiencies and improving the detection rate in diseases. These analyses therefore provide a substantial advantage in applications aimed at the discovery of disease‑specific biomarkers.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Phipps E, Prasanna D, Brima W and Jim B: Preeclampsia: Updates in pathogenesis, definitions and guidelines. Clin J Am Soc Nephrol. 11:1102–1113. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Vikse BE, Irgens LM, Leivestad T, Skjaerven R and Iversen BM: Preeclampsia and the risk of end-stage renal disease. N Engl J Med. 359:800–809. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Drost JT, Maas AH, van Eyck J and van der Schouw YT: Preeclampsia as a female-specific risk factor for chronic hypertension. Maturitas. 67:321–326. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Liu L, Zhang X, Rong C, Rui C, Ji H, Qian YJ, Jia R and Sun L: Distinct DNA methylomes of human placentas between pre-eclampsia and gestational diabetes mellitus. Cell Physiol Biochem. 34:1877–1889. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Ghossein-Doha C, van Neer J, Wissink B, Breetveld NM, de Windt LJ, van Dijk AP, Van Der Vlugt MJ, Janssen MC, Heidema WM, Scholten RR and Spaanderman ME: Preeclampsia, an important female specific risk factor for asymptomatic heart failure. Ultrasound Obstet. Gynecol. 49:143–149. 2016.

6 

Wang A, Rana S and Karumanchi SA: Preeclampsia: The role of angiogenic factors in its pathogenesis. Physiology (Bethesda). 24:147–158. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Baumwell S and Karumanchi SA: Pre-eclampsia: Clinical manifestations and molecular mechanisms. Nephron Clin Pract. 106:C72–C81. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Sankaralingam S, Arenas IA, Lalu MM and Davidge ST: Preeclampsia: Current understanding of the molecular basis of vascular dysfunction. Expert Rev Mol Med. 8:1–20. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Steegers EA, von Dadelszen P, Duvekot JJ and Pijnenborg R: Pre-eclampsia. Lancet. 376:631–644. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Jia R, Li J, Rui C, Ji H, Ding H, Lu Y, De W and Sun L: Comparative Proteomic profile of the human umbilical cord blood exosomes between normal and preeclampsia pregnancies with high-resolution mass spectrometry. Cell Physiol Biochem. 36:2299–2306. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Pillar N, Yoffe L, Hod M and Shomron N: The possible involvement of microRNAs in preeclampsia and gestational diabetes mellitus. Best Pract Res Clin Obstet Gynaecol. 29:176–182. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Zhang YG, Yang HL, Long Y and Li WL: Circular RNA in blood corpuscles combined with plasma protein factor for early prediction of pre-eclampsia. BJOG. 123:2113–2118. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Qian Y, Lu Y, Rui C, Qian Y, Cai M and Jia R: Potential Significance of circular RNA in human placental tissue for patients with preeclampsia. Cell Physiol Biochem. 39:1380–1390. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Hu L, Ye M and Zou H: Recent advances in mass spectrometry-based peptidome analysis. Expert Rev Proteomics. 6:433–447. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Schrader M and Schulz-Knappe P: Peptidomics technologies for human body fluids. Trends Biotechnol. 19:55–60. 2001. View Article : Google Scholar

16 

Xiang Y, Kurokawa MS, Kanke M, Takakuwa Y and Kato T: Peptidomics: Identification of pathogenic and marker peptides. Methods Mol Biol. 615:259–271. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Casalegno-Garduño R, Schmitt A and Schmitt M: Clinical peptide vaccination trials for leukemia patients. Expert Rev Vaccines. 10:785–799. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Tsangaris GT, Karamessinis P, Kolialexi A, Garbis SD, Antsaklis A, Mavrou A and Fountoulakis M: Proteomic analysis of amniotic fluid in pregnancies with Down syndrome. Proteomics. 6:4410–4419. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Li X, Wu LJ, Gu M, Chen YM, Zhang QJ, Li H, Cheng ZJ, Hu P, Han SP, Yu ZB and Qian LM: Peptidomic analysis of amniotic fluid for identification of putative bioactive peptides in ventricular septal defect. Cell Physiol Biochem. 38:1999–2014. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Hsu JL, Huang SY, Chow NH and Chen SH: Stable-isotope dimethyl labeling for quantitative proteomics. Anal Chem. 75:6843–6852. 2004. View Article : Google Scholar

21 

Liu F, Zhao C, Liu L, Ding H, Huo R and Shi Z: Peptidome profiling of umbilical cord plasma associated with gestational diabetes-induced fetal macrosomia. J Proteomics. 139:38–44. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Wan J, Cui XW, Zhang J, Fu ZY, Guo XR, Sun LZ and Ji CB: Peptidome analysis of human skim milk in term and preterm milk. Biochem Biophys Res Commun. 438:236–241. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Cui X, Li Y, Yang L, You L, Wang X, Shi C, Ji C and Guo X: Peptidome analysis of human milk from women delivering macrosomic fetuses reveals multiple means of protection for infants. Oncotarget. 7:63514–63525. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Ghulmiyyah L and Sibai B: Maternal mortality from preeclampsia/eclampsia. Semin Perinatol. 36:56–59. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Shah DM: Preeclampsia: New insights. Curr Opin Nephrol Hypertens. 16:213–220. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Ivanov VT and Yatskin ON: Peptidomics: A logical sequel to proteomics. Expert Rev Proteomics. 2:463–473. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Dallas DC, Guerrero A, Parker EA, Robinson RC, Gan J, German JB, Barile D and Lebrilla CB: Current peptidomics: Applications, purification, identification, quantification, and functional analysis. Proteomics. 15:1026–1038. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Xu RN, Fan L, Rieser MJ and El-Shourbagy TA: Recent advances in high-throughput quantitative bioanalysis by LC-MS/MS. J Pharm Biomed Anal. 44:342–355. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Wang F, Zhu J, Hu L, Qin H, Ye M and Zou H: Comprehensive analysis of the N and C terminus of endogenous serum peptides reveals a highly conserved cleavage site pattern derived from proteolytic enzymes. Protein Cell. 3:669–674. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Gigli M, Begay RL, Morea G, Graw SL, Sinagra G, Taylor MRG, Granzier H and Mestroni L: A review of the giant protein titin in clinical molecular diagnostics of cardiomyopathies. Front Cardiovasc Med. 3:212016. View Article : Google Scholar : PubMed/NCBI

31 

Lindstedt S and Nishikawa K: Huxleys' missing filament: Form and function of titin in vertebrate striated muscle. Annu Rev Physiol. 79:145–166. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Walker JS and de Tombe PP: Titin and the developing heart. Circ Res. 94:860–862. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Liang Y, Lin Q, Luo F, Wu W, Yang T and Wan S: Requirement of miR-144 in CsA induced proliferation and invasion of human trophoblast cells by targeting titin. J Cell Biochem. 115:690–696. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Farina A, Morano D, Arcelli D, De Sanctis P, Sekizawa A, Purwosunu Y, Zucchini C, Simonazzi G, Okai T and Rizzo N: Gene expression in chorionic villous samples at 11 weeks of gestation in women who develop preeclampsia later in pregnancy: Implications for screening. Prenat Diagn. 29:1038–1044. 2009. View Article : Google Scholar : PubMed/NCBI

35 

Araki Y, Nonaka D, Tajima A, Maruyama M, Nitto T, Ishikawa H, Yoshitake H, Yoshida E, Kuronaka N, Asada K, et al: Quantitative peptidomic analysis by a newly developed one-step direct transfer technology without depletion of major blood proteins: Its potential utility for monitoring of pathophysiological status in pregnancy-induced hypertension. Proteomics. 11:2727–2737. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Qian Y, Zhang L, Rui C, Ding H, Mao P, Ruan H and Jia R: Peptidome analysis of amniotic fluid from pregnancies with preeclampsia. Mol Med Rep 16: 7337-7344, 2017.
APA
Qian, Y., Zhang, L., Rui, C., Ding, H., Mao, P., Ruan, H., & Jia, R. (2017). Peptidome analysis of amniotic fluid from pregnancies with preeclampsia. Molecular Medicine Reports, 16, 7337-7344. https://doi.org/10.3892/mmr.2017.7582
MLA
Qian, Y., Zhang, L., Rui, C., Ding, H., Mao, P., Ruan, H., Jia, R."Peptidome analysis of amniotic fluid from pregnancies with preeclampsia". Molecular Medicine Reports 16.5 (2017): 7337-7344.
Chicago
Qian, Y., Zhang, L., Rui, C., Ding, H., Mao, P., Ruan, H., Jia, R."Peptidome analysis of amniotic fluid from pregnancies with preeclampsia". Molecular Medicine Reports 16, no. 5 (2017): 7337-7344. https://doi.org/10.3892/mmr.2017.7582
Copy and paste a formatted citation
x
Spandidos Publications style
Qian Y, Zhang L, Rui C, Ding H, Mao P, Ruan H and Jia R: Peptidome analysis of amniotic fluid from pregnancies with preeclampsia. Mol Med Rep 16: 7337-7344, 2017.
APA
Qian, Y., Zhang, L., Rui, C., Ding, H., Mao, P., Ruan, H., & Jia, R. (2017). Peptidome analysis of amniotic fluid from pregnancies with preeclampsia. Molecular Medicine Reports, 16, 7337-7344. https://doi.org/10.3892/mmr.2017.7582
MLA
Qian, Y., Zhang, L., Rui, C., Ding, H., Mao, P., Ruan, H., Jia, R."Peptidome analysis of amniotic fluid from pregnancies with preeclampsia". Molecular Medicine Reports 16.5 (2017): 7337-7344.
Chicago
Qian, Y., Zhang, L., Rui, C., Ding, H., Mao, P., Ruan, H., Jia, R."Peptidome analysis of amniotic fluid from pregnancies with preeclampsia". Molecular Medicine Reports 16, no. 5 (2017): 7337-7344. https://doi.org/10.3892/mmr.2017.7582
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team