Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
December-2017 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 16 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis

  • Authors:
    • Long Wang
    • Ya‑Qian Hu
    • Zhuo‑Jie Zhao
    • Hong‑Yang Zhang
    • Bo Gao
    • Wei‑Guang Lu
    • Xiao‑Long Xu
    • Xi‑Sheng Lin
    • Jin‑Peng Wang
    • Qiang Jie
    • Zhuo‑Jing Luo
    • Liu Yang
  • View Affiliations / Copyright

    Affiliations: Institute of Orthopedic Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 8427-8433
    |
    Published online on: September 26, 2017
       https://doi.org/10.3892/mmr.2017.7620
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Postmenopausal osteoporosis is one of the most prominent worldwide public health problems and the morbidity is increasing with the aging population. It has been demonstrated that early diagnosis and intervention delay the disease progression and improve the outcome. Therefore, searching for biomarkers that are able to identify postmenopausal women at high risk for developing osteoporosis is an effective way to improve the quality of life of patients, and alleviate social and economic burdens. In the present study, a protein array was used to identify potential biomarkers. The bone mineral densities of 10 rats were dynamically measured in an ovariectomized model by micro‑computed tomography assessment, and the early stage of osteoporosis was defined. Through the protein array‑based screening, the expression levels of six serum protein biomarkers in ovariectomized rats were observed to alter at the initiation stage of the postmenopausal osteoporosis. Fractalkine, tissue inhibitor of metalloproteinases‑1 and monocyte chemotactic protein‑1 were finally demonstrated to be increased in the serum of eight enrolled postmenopausal osteoporosis patients using ELISA assay and were correlated with the severity of progressive bone loss. These biomarkers may be explored as potential early biomarkers to readily evaluate and diagnose postmenopausal osteoporosis in the clinic.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Kanis JA, Melton LJ III, Christiansen C, Johnston CC and Khaltaev N: The diagnosis of osteoporosis. J Bone Miner Res. 9:1137–1141. 1994. View Article : Google Scholar : PubMed/NCBI

2 

Consensus development conference: Diagnosis, prophylaxis and treatment of osteoporosis. Am J Med. 94:646–650. 1993. View Article : Google Scholar : PubMed/NCBI

3 

Mediati RD, Vellucci R and Dodaro L: Pathogenesis and clinical aspects of pain in patients with osteoporosis. Clin Cases Miner Bone Metab. 11:169–172. 2014.PubMed/NCBI

4 

Nagae M, Hiraga T, Wakabayashi H, Wang L, Iwata K and Yoneda T: Osteoclasts play a part in pain due to the inflammation adjacent to bone. Bone. 39:1107–1115. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Roth T, Kammerlander C, Gosch M, Luger TJ and Blauth M: Outcome in geriatric fracture patients and how it can be improved. Osteoporos Int. 21 Suppl 4:S615–S619. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Rosen CJ: Clinical practice. Postmenopausal osteoporosis. N Engl J Med. 353:595–603. 2005. View Article : Google Scholar : PubMed/NCBI

7 

McCormick RK: Osteoporosis: Integrating biomarkers and other diagnostic correlates into the management of bone fragility. Altern Med Rev. 12:113–145. 2007.PubMed/NCBI

8 

Vasikaran S, Eastell R, Bruyère O, Foldes AJ, Garnero P, Griesmacher A, McClung M, Morris HA, Silverman S, Trenti T, et al: Markers of bone turnover for the prediction of fracture risk and monitoring of osteoporosis treatment: A need for international reference standards. Osteoporos Int. 22:391–420. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Kassahun K, McIntosh I, Koeplinger K, Sun L, Talaty JE, Miller DL, Dixon R, Zajic S and Stoch SA: Disposition and metabolism of the cathepsin K inhibitor odanacatib in humans. Drug Metab Dispos. 42:818–827. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Li X, Ominsky MS, Niu QT, Sun N, Daugherty B, D'Agostin D, Kurahara C, Gao Y, Cao J, Gong J, et al: Targeted deletion of the sclerostin gene in mice results in increased bone formation and bone strength. J Bone Miner Res. 23:860–869. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Wang H, Yoshiko Y, Yamamoto R, Minamizaki T, Kozai K, Tanne K, Aubin JE and Maeda N: Overexpression of fibroblast growth factor 23 suppresses osteoblast differentiation and matrix mineralization in vitro. J Bone Miner Res. 23:939–948. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Wang Y, Li L, Moore BT, Peng XH, Fang X, Lappe JM, Recker RR and Xiao P: MiR-133a in human circulating monocytes: A potential biomarker associated with postmenopausal osteoporosis. PLoS One. 7:e346412012. View Article : Google Scholar : PubMed/NCBI

13 

Cao Z, Moore BT, Wang Y, Peng XH, Lappe JM, Recker RR and Xiao P: MiR-422a as a potential cellular microRNA biomarker for postmenopausal osteoporosis. PLoS One. 9:e970982014. View Article : Google Scholar : PubMed/NCBI

14 

Cahill DJ: Protein and antibody arrays and their medical applications. J Immunol Methods. 250:81–91. 2001. View Article : Google Scholar : PubMed/NCBI

15 

Senior K: Fingerprinting disease with protein chip arrays. Mol Med Today. 5:326–327. 1999. View Article : Google Scholar : PubMed/NCBI

16 

Austen BM, Frears ER and Davies H: The use of seldi proteinchip arrays to monitor production of Alzheimer's betaamyloid in transfected cells. J Pept Sci. 6:459–469. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Park SB, Lee YJ and Chung CK: Bone Mineral Density Changes after Ovariectomy in Rats as an Osteopenic Model: Stepwise Description of Double Dorso-Lateral Approach. J Korean Neurosurg Soc. 48:309–312. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Yoshie O, Imai T and Nomiyama H: Chemokines in immunity. Adv Immunol. 78:57–110. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Imai T, Hieshima K, Haskell C, Baba M, Nagira M, Nishimura M, Kakizaki M, Takagi S, Nomiyama H, Schall TJ and Yoshie O: Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91:521–530. 1997. View Article : Google Scholar : PubMed/NCBI

20 

Fong AM, Robinson LA, Steeber DA, Tedder TF, Yoshie O, Imai T and Patel DD: Fractalkine and CX3CR1 mediate a novel mechanism of leukocyte capture, firm adhesion and activation under physiologic flow. J Exp Med. 188:1413–1419. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Han KH, Ryu JW, Lim KE, Lee SH, Kim Y, Hwang CS, Choi JY and Han KO: Vascular expression of the chemokine CX3CL1 promotes osteoclast recruitment and exacerbates bone resorption in an irradiated murine model. Bone. 61:91–101. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Koizumi K, Saitoh Y, Minami T, Takeno N, Tsuneyama K, Miyahara T, Nakayama T, Sakurai H, Takano Y and Nishimura M: Role of CX3CL1/fractalkine in osteoclast differentiation and bone resorption. J Immunol. 183:7825–7831. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Hoshino A, Ueha S, Hanada S, Imai T, Ito M, Yamamoto K, Matsushima K, Yamaguchi A and Iimura T: Roles of chemokine receptor CX3CR1 in maintaining murine bone homeostasis through the regulation of both osteoblasts and osteoclasts. J Cell Sci. 126:1032–1045. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Chen YD, Huang CY, Liu HY, Yao WF, Wu WG, Lu YL and Wang W: Serum CX3CL1/fractalkine concentrations are positively associated with disease severity in postmenopausal osteoporotic patients. Br J Biomed Sci. 73:121–128. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Rifas L, Fausto A, Scott MJ, Avioli LV and Welgus HG: Expression of metalloproteinases and tissue inhibitors of metalloproteinases in human osteoblast-like cells: Differentiation is associated with repression of metalloproteinase biosynthesis. Endocrinology. 134:213–221. 1994. View Article : Google Scholar : PubMed/NCBI

26 

Murphy E, Roux-Lombard P, Rooney T, Fitzgerald O, Dayer JM and Bresnihan B: Serum levels of tissue inhibitor of metalloproteinase-1 and periarticular bone loss in early rheumatoid arthritis. Clin Rheumatol. 28:285–291. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Stanojkovic I, Kotur-Stevuljevic J, Spasic S, Milenkovic B, Vujic T, Stefanovic A and Ivanisevic J: Relationship between bone resorption, oxidative stress and inflammation in severe COPD exacerbation. Clin Biochem. 46:1678–1682. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Ginaldi L, Di Benedetto MC and De Martinis M: Osteoporosis, inflammation and ageing. Immun Ageing. 2:142005. View Article : Google Scholar : PubMed/NCBI

29 

Luo XH, Guo LJ, Shan PF, Xie H, Wu XP, Zhang H, Cao XZ, Yuan LQ and Liao EY: Relationship of circulating MMP-2, MMP-1, and TIMP-1 levels with bone biochemical markers and bone mineral density in postmenopausal Chinese women. Osteoporos Int. 17:521–526. 2006. View Article : Google Scholar : PubMed/NCBI

30 

Eraltan H, Cacina C, Kahraman OT, Kurt O, Aydogan HY, Uyar M, Can A and Cakmakoğlu B: MCP-1 and CCR2 gene variants and the risk for osteoporosis and osteopenia. Genet Test Mol Biomarkers. 16:229–233. 2012. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang L, Hu YQ, Zhao ZJ, Zhang HY, Gao B, Lu WG, Xu XL, Lin XS, Wang JP, Jie Q, Jie Q, et al: Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis. Mol Med Rep 16: 8427-8433, 2017.
APA
Wang, L., Hu, Y., Zhao, Z., Zhang, H., Gao, B., Lu, W. ... Yang, L. (2017). Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis. Molecular Medicine Reports, 16, 8427-8433. https://doi.org/10.3892/mmr.2017.7620
MLA
Wang, L., Hu, Y., Zhao, Z., Zhang, H., Gao, B., Lu, W., Xu, X., Lin, X., Wang, J., Jie, Q., Luo, Z., Yang, L."Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis". Molecular Medicine Reports 16.6 (2017): 8427-8433.
Chicago
Wang, L., Hu, Y., Zhao, Z., Zhang, H., Gao, B., Lu, W., Xu, X., Lin, X., Wang, J., Jie, Q., Luo, Z., Yang, L."Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis". Molecular Medicine Reports 16, no. 6 (2017): 8427-8433. https://doi.org/10.3892/mmr.2017.7620
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Hu YQ, Zhao ZJ, Zhang HY, Gao B, Lu WG, Xu XL, Lin XS, Wang JP, Jie Q, Jie Q, et al: Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis. Mol Med Rep 16: 8427-8433, 2017.
APA
Wang, L., Hu, Y., Zhao, Z., Zhang, H., Gao, B., Lu, W. ... Yang, L. (2017). Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis. Molecular Medicine Reports, 16, 8427-8433. https://doi.org/10.3892/mmr.2017.7620
MLA
Wang, L., Hu, Y., Zhao, Z., Zhang, H., Gao, B., Lu, W., Xu, X., Lin, X., Wang, J., Jie, Q., Luo, Z., Yang, L."Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis". Molecular Medicine Reports 16.6 (2017): 8427-8433.
Chicago
Wang, L., Hu, Y., Zhao, Z., Zhang, H., Gao, B., Lu, W., Xu, X., Lin, X., Wang, J., Jie, Q., Luo, Z., Yang, L."Screening and validation of serum protein biomarkers for early postmenopausal osteoporosis diagnosis". Molecular Medicine Reports 16, no. 6 (2017): 8427-8433. https://doi.org/10.3892/mmr.2017.7620
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team