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Article

Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro

  • Authors:
    • Wei‑Min Zhang
    • Zhi‑Ren Zhang
    • Xi‑Tao Yang
    • Yong‑Gang Zhang
    • Yan‑Sheng Gao
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Zhumadian Central Hospital, Zhumadian, Henan 463000, P.R. China, Medical Department, Zhumadian Central Hospital, Zhumadian, Henan 463000, P.R. China, Department of Interventional Radiotherapy, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, P.R. China
  • Pages: 330-335
    |
    Published online on: October 24, 2017
       https://doi.org/10.3892/mmr.2017.7856
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Abstract

The primary aim of the present study was to examine the effects of microRNA‑21 (miR‑21) on the proliferation and differentiation of rat primary neural stem cells (NSCs) in vitro. miR‑21 was overexpressed in NSCs by transfection with a miR‑21 mimic. The effects of miR‑21 overexpression on NSC proliferation were revealed by Cell Counting kit 8 and 5‑ethynyl‑2'‑deoxyuridine incorporation assay, and miR‑21 overexpression was revealed to increase NSC proliferation. miR‑21 overexpression was confirmed using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). mRNA and protein expression levels of key molecules (β‑catenin, cyclin D1, p21 and miR‑21) in the Wnt/β‑catenin signaling pathway were studied by RT‑qPCR and western blot analysis. RT‑qPCR and western blot analyses revealed that miR‑21 overexpression increased β‑catenin and cyclin D1 expression, and decreased p21 expression. These results suggested that miR‑21‑induced increase in proliferation was mediated by activation of the Wnt/β‑catenin signaling pathway, since overexpression of miR‑21 increased β‑catenin and cyclin D1 expression and reduced p21 expression. Furthermore, inhibition of the Wnt/β‑catenin pathway with FH535 attenuated the influence of miR‑21 overexpression on NSC proliferation, indicating that the factors activated by miR‑21 overexpression were inhibited by FH535 treatment. Furthermore, overexpression of miR‑21 enhanced the differentiation of NSCs into neurons and inhibited their differentiation into astrocytes. The present study indicated that in primary rat NSCs, overexpression of miR‑21 may promote proliferation and differentiation into neurons via the Wnt/β‑catenin signaling pathway in vitro.
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1 

Aranha MM, Santos DM, Solá S, Steer CJ and Rodrigues CM: miR-34a regulates mouse neural stem cell differentiation. PLoS One. 6:e213962011. View Article : Google Scholar : PubMed/NCBI

2 

Shi Y, Sun G, Zhao C and Stewart R: Neural stem cell self-renewal. Crit Rev Oncol Hematol. 65:43–53. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Koliatsos VE, Xu L and Cummings BJ: Stem cell therapies for traumatic brain injury. Regen Med. 10:917–920. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Bi YY, Feng DF and Pan DC: Stem/progenitor cells: A potential source of retina-specific cells for retinal repair. Neurosci Res. 65:215–221. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Piltti KM, Avakian SN, Funes GM, Hu A, Uchida N, Anderson AJ and Cummings BJ: Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury. Stem Cell Res. 15:341–353. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Lu H, Li M, Song T, Qian Y, Xiao X, Chen X, Zhang P, Feng X, Parker T and Liu Y: Retrovirus delivered neurotrophin-3 promotes survival, proliferation and neuronal differentiation of human fetal neural stem cells in vitro. Brain Res Bull. 77:158–164. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Zhou XM, Sun JB, Yuan HP, Wu DL, Zhou XR, Sun DW, Li HY, Shao ZB and Zhang ZR: A rat model for studying neural stem cell transplantation. Acta Pharmacol Sin. 30:1496–1504. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Luo Y and Zhu D: Combinatorial control of transgene expression by hypoxia-responsive promoter and microrna regulation for neural stem cell-based cancer therapy. Biomed Res Int. 2014:7513972014. View Article : Google Scholar : PubMed/NCBI

9 

Lopez-Ramirez MA and Nicoli S: Role of miRNAs and epigenetics in neural stem cell fate determination. Epigenetics. 9:90–100. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Rolando C and Taylor V: Neural stem cell of the hippocampus: Development, physiology regulation, and dysfunction in disease. Curr Top Dev Biol. 107:183–206. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Xia T, Li J, Cheng H, Zhang C and Zhang Y: Small-molecule regulators of MicroRNAs in biomedicine. Drug Dev Res. 76:375–381. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Chen B, Li H, Zeng X, Yang P, Liu X, Zhao X and Liang S: Roles of microRNA on cancer cell metabolism. J Transl Med. 10:2282012. View Article : Google Scholar : PubMed/NCBI

13 

Iwakawa HO and Tomari Y: The functions of MicroRNAs: mRNA decay and translational repression. Trends Cell Biol. 25:651–665. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Huang J, Zhang SY, Gao YM, Liu YF, Liu YB, Zhao ZG and Yang K: MicroRNAs as oncogenes or tumour suppressors in oesophageal cancer: Potential biomarkers and therapeutic targets. Cell Prolif. 47:277–286. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Fang S, Deng Y, Gu P and Fan X: MicroRNAs regulate bone development and regeneration. Int J Mol Sci. 16:8227–8253. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Li J, You T and Jing J: MiR-125b inhibits cell biological progression of Ewing's sarcoma by suppressing the PI3K/Akt signalling pathway. Cell Prolif. 47:152–160. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Li M, Yu M, Liu C, Zhu H, He X, Peng S and Hua J: miR-34c works downstream of p53 leading to dairy goat male germline stem-cell (mGSCs) apoptosis. Cell Prolif. 46:223–231. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Gangaraju VK and Lin H: MicroRNAs: Key regulators of stem cells. Nat Rev Mol Cell Biol. 10:116–125. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Choi E, Choi E and Hwang KC: MicroRNAs as novel regulators of stem cell fate. World J Stem Cells. 5:172–187. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Zhu W, Mao Y, Zhao Y, Zhou LF, Wang Y, Zhu JH, Zhu Y and Yang GY: Transplantation of vascular endothelial growth factor-transfected neural stem cells into the rat brain provides neuroprotection after transient focal cerebral ischemia. Neurosurgery. 57:325–333. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methds. 25:402–408. 2001. View Article : Google Scholar

22 

Xiong Y, Hannon GJ, Zhang H, Casso D, Kobayashi R and Beach D: p21 is a universal inhibitor of cyclin kinases. Nature. 366:701–704. 1993. View Article : Google Scholar : PubMed/NCBI

23 

Sherr CJ: D-type cyclins. Trends Biochem Sci. 20:187–190. 1995. View Article : Google Scholar : PubMed/NCBI

24 

Wu D, Shi M and Fan XD: Mechanism of miR-21 via Wnt/β-catenin signaling pathway in human A549 lung cancer cells and Lewis lung carcinoma in mice. Asian Pac J Trop Med. 8:479–484. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Liu XJ, Hong Q, Wang Z, Yu YY, Zou X and Xu LH: MicroRNA21 promotes interstitial fibrosis via targeting DDAH1: A potential role in renal fibrosis. Mol Cell Biochem. 411:181–199. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Shi C, Yang Y, Xia Y, Okugawa Y, Yang J, Liang Y, Chen H, Zhang P, Wang F, Han H, et al: Novel evidence for an oncogenic role of microRNA-21 in colitis-associated colorectal cancer. Gut. 65:1470–1481. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Yu Y, Nangia-Makker P, Farhana LG, Rajendra S, Levi E and Majumdar AP: miR-21 and miR-145 cooperation in regulation of colon cancer stem cells. Mol Cancer. 14:982015. View Article : Google Scholar : PubMed/NCBI

28 

Munji RN, Choe Y, Li G, Siegenthaler JA and Pleasure SJ: Wnt signaling regulates neuronal differentiation of cortical intermediate progenitors. J Neurosci. 31:1676–1687. 2011. View Article : Google Scholar : PubMed/NCBI

29 

De Ferrari GV, Avila ME, Medina MA, Perez-Palma E, Bustos BI and Alarcon MA: Wnt/β-catenin signaling in Alzheimer's disease. CNS Neurol Disord Drug Targets. 13:745–754. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Mazur M, Bujak A, Matloka M, Janowska S, Gunerka P, Bojarski L, Stanczak A, Klejman A, Bednarek A, Lamparska-Przybysz M and Wieczorek M: Cell-based assay for low-and high-scale screening of the Wnt/β-catenin signaling modulators. Anal Biochem. 475:56–67. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Sun FL, Wang W, Zuo W, Xue JL, Xu JD, Ai HX, Zhang L, Wang XM and Ji XM: Promoting neurogenesis via Wnt/β-catenin signaling pathway accounts for the neurorestorative effects of morroniside against cerebral ischemia injury. Eur J Pharmacol. 738:214–221. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Jamieson C, Sharma M and Henderson BR: Wnt signaling from membrane to nucleus: β-catenin caught in a loop. Int J Biochem Cell Biol. 44:847–850. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang WM, Zhang ZR, Yang XT, Zhang YG and Gao YS: Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro. Mol Med Rep 17: 330-335, 2018.
APA
Zhang, W., Zhang, Z., Yang, X., Zhang, Y., & Gao, Y. (2018). Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro. Molecular Medicine Reports, 17, 330-335. https://doi.org/10.3892/mmr.2017.7856
MLA
Zhang, W., Zhang, Z., Yang, X., Zhang, Y., Gao, Y."Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro". Molecular Medicine Reports 17.1 (2018): 330-335.
Chicago
Zhang, W., Zhang, Z., Yang, X., Zhang, Y., Gao, Y."Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro". Molecular Medicine Reports 17, no. 1 (2018): 330-335. https://doi.org/10.3892/mmr.2017.7856
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang WM, Zhang ZR, Yang XT, Zhang YG and Gao YS: Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro. Mol Med Rep 17: 330-335, 2018.
APA
Zhang, W., Zhang, Z., Yang, X., Zhang, Y., & Gao, Y. (2018). Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro. Molecular Medicine Reports, 17, 330-335. https://doi.org/10.3892/mmr.2017.7856
MLA
Zhang, W., Zhang, Z., Yang, X., Zhang, Y., Gao, Y."Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro". Molecular Medicine Reports 17.1 (2018): 330-335.
Chicago
Zhang, W., Zhang, Z., Yang, X., Zhang, Y., Gao, Y."Overexpression of miR‑21 promotes neural stem cell proliferation and neural differentiation via the Wnt/β‑catenin signaling pathway in vitro". Molecular Medicine Reports 17, no. 1 (2018): 330-335. https://doi.org/10.3892/mmr.2017.7856
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