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Article

GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways

  • Authors:
    • Ming‑Zhen Wang
    • Cheng‑Zhi Qiu
    • Wai‑Shi Yu
    • Yan‑Ta Guo
    • Chun‑Xiao Wang
    • Zhi‑Xiong Chen
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, Department of Gastroenterology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China
  • Pages: 542-548
    |
    Published online on: October 25, 2017
       https://doi.org/10.3892/mmr.2017.7877
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Abstract

The novel proto‑oncogene Golgi phosphoprotein (GOLPH)3 is overexpressed in a variety of tumor tissues and is associated with poor prognosis. The authors previously demonstrated that GOLPH3 gene is overexpressed in colorectal cancer tissues and promotes the proliferation of colonic cancer cells by activating the phosphatidylinositol‑3‑kinase/protein kinase B/the mammalian target of rapamycin and Wnt/β‑catenin signaling pathways. However, to the best of the authors' knowledge, if and how the GOLPH3 gene is involved in inducing resistance to colonic cancer chemotherapy has not been reported. In the present study, the association between the overexpression of the GOLPH3 gene and resistance of HT29 colonic cancer cells to 5‑fluorouracil (5‑FU) was investigated. Following confirmation of the effective silencing of the GOLPH3 gene, proliferation and apoptosis of colonic cancer cells were detected by MTT assay, colony formation assay and flow cytometry, and then the mechanism of GOLPH3‑induced resistance to 5‑FU chemotherapy in colonic cancer cells was investigated by western blotting. The results demonstrated that the expression of phosphorylated (p)‑glycoprotein and GOLPH3 was increased in HT29 cells following treatment with 5‑FU, which resulted in the development of drug resistance. Silencing GOLPH3 increased the sensitivity of HT29 cells to 5‑FU, reduced their tumorigenicity and partly reversed their resistance to 5‑FU. The expression of p‑extracellular signal‑regulated kinase (pERK)1/2 and β‑catenin was decreased, which indicated that its mechanism was associated with the activation of the mitogen‑activated protein kinase/ERK and Wnt/β‑catenin signaling pathways. Therefore, GOLPH3 may be a potential, novel target for reversing chemotherapy resistance in colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Wang MZ, Qiu CZ, Yu WS, Guo YT, Wang CX and Chen ZX: GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways. Mol Med Rep 17: 542-548, 2018.
APA
Wang, M., Qiu, C., Yu, W., Guo, Y., Wang, C., & Chen, Z. (2018). GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways. Molecular Medicine Reports, 17, 542-548. https://doi.org/10.3892/mmr.2017.7877
MLA
Wang, M., Qiu, C., Yu, W., Guo, Y., Wang, C., Chen, Z."GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways". Molecular Medicine Reports 17.1 (2018): 542-548.
Chicago
Wang, M., Qiu, C., Yu, W., Guo, Y., Wang, C., Chen, Z."GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways". Molecular Medicine Reports 17, no. 1 (2018): 542-548. https://doi.org/10.3892/mmr.2017.7877
Copy and paste a formatted citation
x
Spandidos Publications style
Wang MZ, Qiu CZ, Yu WS, Guo YT, Wang CX and Chen ZX: GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways. Mol Med Rep 17: 542-548, 2018.
APA
Wang, M., Qiu, C., Yu, W., Guo, Y., Wang, C., & Chen, Z. (2018). GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways. Molecular Medicine Reports, 17, 542-548. https://doi.org/10.3892/mmr.2017.7877
MLA
Wang, M., Qiu, C., Yu, W., Guo, Y., Wang, C., Chen, Z."GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways". Molecular Medicine Reports 17.1 (2018): 542-548.
Chicago
Wang, M., Qiu, C., Yu, W., Guo, Y., Wang, C., Chen, Z."GOLPH3 expression promotes the resistance of HT29 cells to 5‑fluorouracil by activating multiple signaling pathways". Molecular Medicine Reports 17, no. 1 (2018): 542-548. https://doi.org/10.3892/mmr.2017.7877
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