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Article

Adiponectin promotes preadipocyte differentiation via the PPARγ pathway

  • Authors:
    • Wenkai Yang
    • Chunli Yang
    • Jun Luo
    • Yutao Wei
    • Wenying Wang
    • Yingmei Zhong
  • View Affiliations / Copyright

    Affiliations: Department of Cardio‑Thoracic Surgery, Ganzhou People's Hospital, Ganzhou, Jiangxi 341000, P.R. China, Department of Intensive Care, Jiangxi People's Hospital, Nanchang, Jiangxi 330006, P.R. China, Department of Cardio‑Thoracic Surgery, The First Affiliated Hospital of Shihezi University Medical College, Shihezi, Xinjiang 832008, P.R. China
  • Pages: 428-435
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    Published online on: October 26, 2017
       https://doi.org/10.3892/mmr.2017.7881
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Abstract

According to the results of a preliminary study, it was hypothesized that the effects of adiponectin (APN) on the improvement of atherosclerosis may be associated with adipocyte differentiation and peroxisome proliferator‑activated receptor γ (PPARγ). The present study simulated the inflammatory environment of epicardial adipose tissue by stimulating mature adipocytes with lipopolysaccharide (LPS); subsequently, the differentiation of 3T3‑L1 preadipocytes was observed. 3T3‑L1 preadipocytes were infected with an adenovirus containing the human adiponectin gene apM1 (Ad‑apM1) and were co‑cultured with mature adipocytes stimulated with LPS. Differentiation into mature adipocytes was initiated using differentiation medium. After 8 days, an MTT assay was used to examine cell viability and oil red O staining was used to observe preadipocyte differentiation. In addition, the mRNA expression levels of monocyte chemoattractant protein‑1 (MCP‑1), interleukin (IL)‑6, IL‑8 and tumor necrosis factor α (TNF‑α) were examined by quantitative polymerase chain reaction, and the protein expression levels of PPARγ, CCAAT/enhancer binding protein α (C/EBPα) and preadipocyte factor‑1 (Pref‑1) were measured by western blotting. The results indicated that APN overexpression significantly increased preadipocyte differentiation and cell viability, inhibited MCP‑1, IL‑6, IL‑8 and TNF‑α expression, upregulated PPARγ and C/EBPα expression, and downregulated Pref‑1 under LPS stimulation. In addition, inhibition of PPARγ activity by T0070907 markedly attenuated the effects of APN overexpression. Taken together, the present study demonstrated that the effects of APN on the promotion of preadipocyte differentiation under inflammatory conditions may involve the PPARγ signaling pathway, and at least partly depends on upregulation of PPARγ expression.
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Spandidos Publications style
Yang W, Yang C, Luo J, Wei Y, Wang W and Zhong Y: Adiponectin promotes preadipocyte differentiation via the PPARγ pathway. Mol Med Rep 17: 428-435, 2018.
APA
Yang, W., Yang, C., Luo, J., Wei, Y., Wang, W., & Zhong, Y. (2018). Adiponectin promotes preadipocyte differentiation via the PPARγ pathway. Molecular Medicine Reports, 17, 428-435. https://doi.org/10.3892/mmr.2017.7881
MLA
Yang, W., Yang, C., Luo, J., Wei, Y., Wang, W., Zhong, Y."Adiponectin promotes preadipocyte differentiation via the PPARγ pathway". Molecular Medicine Reports 17.1 (2018): 428-435.
Chicago
Yang, W., Yang, C., Luo, J., Wei, Y., Wang, W., Zhong, Y."Adiponectin promotes preadipocyte differentiation via the PPARγ pathway". Molecular Medicine Reports 17, no. 1 (2018): 428-435. https://doi.org/10.3892/mmr.2017.7881
Copy and paste a formatted citation
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Spandidos Publications style
Yang W, Yang C, Luo J, Wei Y, Wang W and Zhong Y: Adiponectin promotes preadipocyte differentiation via the PPARγ pathway. Mol Med Rep 17: 428-435, 2018.
APA
Yang, W., Yang, C., Luo, J., Wei, Y., Wang, W., & Zhong, Y. (2018). Adiponectin promotes preadipocyte differentiation via the PPARγ pathway. Molecular Medicine Reports, 17, 428-435. https://doi.org/10.3892/mmr.2017.7881
MLA
Yang, W., Yang, C., Luo, J., Wei, Y., Wang, W., Zhong, Y."Adiponectin promotes preadipocyte differentiation via the PPARγ pathway". Molecular Medicine Reports 17.1 (2018): 428-435.
Chicago
Yang, W., Yang, C., Luo, J., Wei, Y., Wang, W., Zhong, Y."Adiponectin promotes preadipocyte differentiation via the PPARγ pathway". Molecular Medicine Reports 17, no. 1 (2018): 428-435. https://doi.org/10.3892/mmr.2017.7881
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