Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen

  • Authors:
    • De‑Wang Wang
    • Wei‑Wei Ni
    • Yan‑Jun Zhou
    • Wen Huang
    • Meng‑Da Cao
    • Ling Meng
    • Ji‑Fu Wei
  • View Affiliations / Copyright

    Affiliations: Research Division of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 394-399
    |
    Published online on: October 26, 2017
       https://doi.org/10.3892/mmr.2017.7883
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Platanus acerifolia is one of the major sources of outdoor allergens to humans, and can induce allergic asthma, rhinitis, dermatitis and other allergic diseases. Pla a 2 is a polygalacturonase and represents the major allergen identified in P. acerifolia pollen. The aim of the present study was to express and purify Pla a 2, and to predict B and T cell epitopes of Pla a 2. The gene encoding Pla a 2 was cloned into the pET28a vector and subsequently transfected into ArcticExpress™ (DE3) Escherichia coli cells; purified Pla a 2 was analyzed by western blot analysis. The results of the present study revealed that the Pla a 2 allergen has the ability to bind immunoglobulin E within the sera of patients allergic to P. acerifolia pollen. In addition, the B cell epitopes of Pla a 2 were predicted using the DNAStar Protean system, Bioinformatics Predicted Antigenic Peptides and BepiPred 1.0 software; T cell epitopes were predicted using NetMHCIIpan ‑3.0 and ‑2.2. In total, eight B cell epitopes (15‑24, 60‑66, 78‑86, 109‑124, 232‑240, 260‑269, 298‑306 and 315‑322) and five T cell epitopes (62‑67, 86‑91, 125‑132, 217‑222 and 343‑350) were predicted in the present study. These findings may be used to improve allergen immunotherapies and reduce the frequency of pollen‑associated allergic reactions.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Malmstrom K, Kaila M, Kajosaari M, Syvanen P and Juntunen-Backman K: Fatal asthma in Finnish children and adolescents 1976–1998: validity of death certificates and a clinical description. Pediatr Pulmonol. 42:210–215. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Sullivan PW, Smith KL, Ghushchyan VH, Globe DR, Lin SL and Globe G: Asthma in USA: Its impact on health-related quality of life. J Asthma. 50:891–899. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Moorman JE, Rudd RA, Johnson CA, King M, Minor P, Bailey C, Scalia MR and Akinbami LJ: National surveillance for asthma-United States, 1980–2004. MMWR Surveill Summ. 56:1–54. 2007.PubMed/NCBI

4 

Snijders BE, Thijs C, Dagnelie PC, Stelma FF, Mommers M, Kummeling I, Penders J, van Ree R and van den Brandt PA: Breast-feeding duration and infant atopic manifestations, by maternal allergic status, in the first 2 years of life (KOALA study). J Pediatr. 151(347–351): 351. e341–342. 2007.

5 

Hendaus MA, Jomha FA and Ehlayel M: Allergic diseases among children: nutritional prevention and intervention. Ther Clin Risk Manag. 12:361–372. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Warner JO, Kaliner MA, Crisci CD, Del Giacco S, Frew AJ, Liu GH, Maspero J, Moon HB, Nakagawa T, Potter PC, et al: Allergy practice worldwide: A report by the world allergy organization specialty and training council. Int Arch Allergy Immunol. 139:166–174. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Alcazar P, Garcia-Mozo H, Mdel Trigo M, Ruiz L, Gonzalez-Minero FJ, Hidalgo P, de la Guardia Diaz C and Galan C: Platanus pollen season in Andalusia (southern Spain): Trends and modeling. J Environ Monit. 13:2502–2510. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Fernandez-Gonzalez D, Gonzalez-Parrado Z, Vega-Maray AM, Valencia-Barrera RM, Camazon-Izquierdo B, De Nuntiis P and Mandrioli P: Platanus pollen allergen, Pla a 1: Quantification in the atmosphere and influence on a sensitizing population. Clinical and experimental allergy. J Br Soc Allergy Clin Immunol. 40:1701–1708. 2010. View Article : Google Scholar

9 

Lauer I, Miguel-Moncin MS, Abel T, Foetisch K, Hartz C, Fortunato D, Cistero-Bahima A, Vieths S and Scheurer S: Identification of a plane pollen lipid transfer protein (Pla a 3) and its immunological relation to the peach lipid-transfer protein, Pru p 3. Clin Exp Allergy. 37:261–269. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Ibarrola I, Arilla MC, Martinez A and Asturias JA: Identification of a polygalacturonase as a major allergen (Pla a 2) from Platanus acerifolia pollen. J Allergy Clin Immunol. 113:1185–1191. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Wangorsch A, Larsson H, Messmer M, Garcia-Moral A, Lauer I, Wolfheimer S, Schulke S, Bartra J, Vieths S, Lidholm J, et al: Molecular cloning of plane pollen allergen Pla a 3 and its utility as diagnostic marker for peach associated plane pollen allergy. Clin Exp Allergy. 46:764–774. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Abramson MJ, Puy RM and Weiner JM: Allergen immunotherapy for asthma. Cochrane Database Syst Rev Cd001186. 2003. View Article : Google Scholar

13 

Vadlamudi A and Shaker M: New developments in allergen immunotherapy. Curr Opin Pediatr. 27:649–655. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Calderon MA, Alves B, Jacobson M, Hurwitz B, Sheikh A and Durham S: Allergen injection immunotherapy for seasonal allergic rhinitis. Cochrane Database Syst Rev Cd001936. 2007. View Article : Google Scholar

15 

Dahl R, Kapp A, Colombo G, de Monchy JG, Rak S, Emminger W, Rivas MF, Ribel M and Durham SR: Efficacy and safety of sublingual immunotherapy with grass allergen tablets for seasonal allergic rhinoconjunctivitis. J Allergy Clin Immunol. 118:434–440. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Madonini E and Musarra A: Allergen immunotherapy in asthma: Current evidence and future requirements. Eur Ann Allergy Clin Immunol. 43:103–110. 2011.PubMed/NCBI

17 

Kim JM, Lin SY, Suarez-Cuervo C, Chelladurai Y, Ramanathan M, Segal JB and Erekosima N: Allergen-specific immunotherapy for pediatric asthma and rhinoconjunctivitis: A systematic review. Pediatrics. 131:1155–67. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Thomas WR: The advent of recombinant allergens and allergen cloning. J Allergy Clin Immunol. 127:855–859. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Jutel M, Kosowska A and Smolinska S: Allergen immunotherapy: Past, Present, and Future. Allergy Asthma Immunol Res. 8:191–197. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Shendure JA, Porreca GJ, Church GM, Gardner AF, Hendrickson CL, Kieleczawa J and Slatko BE: Overview of DNA sequencing strategies. Curr Protoc Mol Biol Chapter. 7:Unit7.12011.

21 

Minato Y, Ueda T, Machiyama A, Shimada I and Iwai H: Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing. J Biomol NMR. 53:191–207. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Kumar S, Jain KK, Singh A, Panda AK and Kuhad RC: Characterization of recombinant pectate lyase refolded from inclusion bodies generated in E. coli BL21(DE3). Protein Expr Purif. 110:43–51. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Liu Y, Sun X, Wang G, Tao A, Wu Y, Li M, Shi H and Xie M: Expression, purification and identification of Pla a1 in a codon-optimized Platanus pollen allergen. Mol Med Rep. 12:2197–2202. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Zhang Y, Du WX, Fregevu C, Kothary MH, Harden L and McHugh TH: Expression, purification, and characterization of almond (Prunus dulcis) allergen Pru du 4. J Agric Food Chem. 62:12695–12700. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Tong X, Guo M, Jin M, Chen H, Li Y and Wei JF: In silico epitope prediction, expression and functional analysis of Per a 10 allergen from the American cockroach. Int J Mol Med. 38:1806–1814. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Kiefer F, Arnold K, Kunzli M, Bordoli L and Schwede T: The SWISS-MODEL Repository and associated resources. Nucleic Acids Res. 37:D387–392. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Arnold K, Bordoli L, Kopp J and Schwede T: The SWISS-MODEL workspace: A web-based environment for protein structure homology modelling. Bioinformatics. 22:195–201. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Yang H, Chen H, Jin M, Xie H, He S and Wei JF: Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach. Int J Mol Med. 38:1795–1805. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Yang X and Yu X: An introduction to epitope prediction methods and software. Rev Med Virol. 19:77–96. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Zheng LN, Lin H, Pawar R, Li ZX and Li MH: Mapping IgE binding epitopes of major shrimp (Penaeus monodon) allergen with immunoinformatics tools. Food Chem Toxicol. 49:2954–2960. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Nielsen M and Lund O: NN-align. An artificial neural network-based alignment algorithm for MHC class II peptide binding prediction. BMC Bioinformatics. 10:2962009. View Article : Google Scholar : PubMed/NCBI

32 

Karosiene E, Rasmussen M, Blicher T, Lund O, Buus S and Nielsen M: NetMHCIIpan-3.0, a common pan-specific MHC class II prediction method including all three human MHC class II isotypes, HLA-DR, HLA-DP and HLA-DQ. Immunogenetics. 65:711–724. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Chen H, Yang HW, Wei JF and Tao AL: In silico prediction of the T-cell and IgE-binding epitopes of Per a 6 and Bla g 6 allergens in cockroaches. Mol Med Rep. 10:2130–2136. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Goldblum RM, Ning B, Judy BM, Holthauzen LM, van Bavel J, Kamijo A and Midoro-Horiuti T: A single mouse monoclonal antibody, E58 modulates multiple IgE epitopes on group 1 cedar pollen allergens. Mol Immunol. 74:106–112. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Li X, Yang HW, Chen H, Wu J, Liu Y and Wei JF: In silico prediction of T and B cell epitopes of Der f 25 in Dermatophagoides farinae. Int J Genom. 2014:4839052014.

36 

van Ree R: The CREATE project: EU support for the improvement of allergen standardization in Europe. Allergy. 59:571–574. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Asero R, Mistrello G, Amato S and Villalta D: Monosensitization to a novel plane pollen allergen. Eur Ann Allergy Clin Immunol. 44:167–169. 2012.PubMed/NCBI

38 

Enrique E, Alonso R, Bartolome B, San Miguel-Moncin M, Bartra J, Fernandez-Parra B, Tella R, Asturias JA, Ibarrola I, Martínez A, et al: IgE reactivity to profilin in Platanus acerifolia pollen-sensitized subjects with plant-derived food allergy. J Investig Allergol Clin Immunol. 14:335–342. 2004.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang DW, Ni WW, Zhou YJ, Huang W, Cao MD, Meng L and Wei JF: Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen. Mol Med Rep 17: 394-399, 2018.
APA
Wang, D., Ni, W., Zhou, Y., Huang, W., Cao, M., Meng, L., & Wei, J. (2018). Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen. Molecular Medicine Reports, 17, 394-399. https://doi.org/10.3892/mmr.2017.7883
MLA
Wang, D., Ni, W., Zhou, Y., Huang, W., Cao, M., Meng, L., Wei, J."Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen". Molecular Medicine Reports 17.1 (2018): 394-399.
Chicago
Wang, D., Ni, W., Zhou, Y., Huang, W., Cao, M., Meng, L., Wei, J."Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen". Molecular Medicine Reports 17, no. 1 (2018): 394-399. https://doi.org/10.3892/mmr.2017.7883
Copy and paste a formatted citation
x
Spandidos Publications style
Wang DW, Ni WW, Zhou YJ, Huang W, Cao MD, Meng L and Wei JF: Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen. Mol Med Rep 17: 394-399, 2018.
APA
Wang, D., Ni, W., Zhou, Y., Huang, W., Cao, M., Meng, L., & Wei, J. (2018). Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen. Molecular Medicine Reports, 17, 394-399. https://doi.org/10.3892/mmr.2017.7883
MLA
Wang, D., Ni, W., Zhou, Y., Huang, W., Cao, M., Meng, L., Wei, J."Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen". Molecular Medicine Reports 17.1 (2018): 394-399.
Chicago
Wang, D., Ni, W., Zhou, Y., Huang, W., Cao, M., Meng, L., Wei, J."Expression, purification and epitope analysis of Pla a 2 allergen from Platanus acerifolia pollen". Molecular Medicine Reports 17, no. 1 (2018): 394-399. https://doi.org/10.3892/mmr.2017.7883
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team