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Article

In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging

  • Authors:
    • Yong Qin
    • Lisha Zhuo
    • Jinhua Cai
    • Xiaoya He
    • Bo Liu
    • Chuan Feng
    • Lin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Children's Hospital of Chongqing Medical University, Chongqing 400014, P.R. China, Outpatient Department, 77100 Troops, Chinese People's Liberation Army, Chongqing 400020, P.R. China, Department of Radiology, Xinan Hospital of Third Military Medical University, Chongqing 400038, P.R. China
  • Pages: 452-458
    |
    Published online on: October 26, 2017
       https://doi.org/10.3892/mmr.2017.7902
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Abstract

Although mesenchymal stem cells (MSCs) have been demonstrated to possess a tumor‑homing feature, their tropism to liver tumors has not been delineated in a visible manner. The aim of the present study was to evaluate the tumor‑homing capacity of MSCs and to investigate the spatial and temporal distributions of MSCs in liver tumors using magnetic resonance imaging (MRI). MSCs were colabeled with superparamagnetic iron oxide (SPIO) particles and 4',6‑diamidino‑2‑phenylindole (DAPI), and then transplanted into rabbits with VX2 liver tumors through intravenous injections. The rabbits were subjected to MRI before and at 3, 7 and 14 days after cell transplantation using a clinical 1.5‑T MRI system. Immediately after the MRI examination, histological analyses were performed using fluorescence and Prussian blue staining. At 3 days after injection with labeled MSCs, heterogeneous hypointensity was detected on the MRI images of the tumor. At 7 days after transplantation, the tumor exhibited anisointense MRI signal, whereas a hypointense ring was detected at the border of the tumor. At 14 days after transplantation, the MRI signal recovered the hyperintensity. As demonstrated in the histological analyses, the distribution of the iron particles visualized with Prussian blue staining was consistent with the DAPI‑stained bright fluorescent nuclei, and the particles corresponded to the hypointense region on the MR images. Thus, systemically administered MSCs could localize to liver tumors with high specificity and possessed a migration feature with active tumor growth. These results demonstrated that the targeting and distribution of the magnetically labeled stem cells in the tumor could be tracked for 7 days in vivo using a clinical 1.5‑T MRI scanner.
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Copy and paste a formatted citation
Spandidos Publications style
Qin Y, Zhuo L, Cai J, He X, Liu B, Feng C and Zhang L: In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging. Mol Med Rep 17: 452-458, 2018.
APA
Qin, Y., Zhuo, L., Cai, J., He, X., Liu, B., Feng, C., & Zhang, L. (2018). In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging. Molecular Medicine Reports, 17, 452-458. https://doi.org/10.3892/mmr.2017.7902
MLA
Qin, Y., Zhuo, L., Cai, J., He, X., Liu, B., Feng, C., Zhang, L."In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging". Molecular Medicine Reports 17.1 (2018): 452-458.
Chicago
Qin, Y., Zhuo, L., Cai, J., He, X., Liu, B., Feng, C., Zhang, L."In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging". Molecular Medicine Reports 17, no. 1 (2018): 452-458. https://doi.org/10.3892/mmr.2017.7902
Copy and paste a formatted citation
x
Spandidos Publications style
Qin Y, Zhuo L, Cai J, He X, Liu B, Feng C and Zhang L: In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging. Mol Med Rep 17: 452-458, 2018.
APA
Qin, Y., Zhuo, L., Cai, J., He, X., Liu, B., Feng, C., & Zhang, L. (2018). In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging. Molecular Medicine Reports, 17, 452-458. https://doi.org/10.3892/mmr.2017.7902
MLA
Qin, Y., Zhuo, L., Cai, J., He, X., Liu, B., Feng, C., Zhang, L."In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging". Molecular Medicine Reports 17.1 (2018): 452-458.
Chicago
Qin, Y., Zhuo, L., Cai, J., He, X., Liu, B., Feng, C., Zhang, L."In vivo monitoring of magnetically labeled mesenchymal stem cells homing to rabbit hepatic VX2 tumors using magnetic resonance imaging". Molecular Medicine Reports 17, no. 1 (2018): 452-458. https://doi.org/10.3892/mmr.2017.7902
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