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Article

Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol

  • Authors:
    • Yuejiao Wei
    • Tianyu Meng
    • Chaofeng Sun
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 495-501
    |
    Published online on: October 26, 2017
       https://doi.org/10.3892/mmr.2017.7906
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Abstract

The aim of the present study was to analyze the effect of diltiazem on myocardial fibrosis and remodeling of connexin43 (Cx43) in myocardial ischemic rats and mechanisms underlying these processes. A total of 36 Sprague‑Dawley rats were randomly allocated into three groups (control, isoproterenol and isoproterenol with diltiazem). The myocardial ischemic model was established by 5 mg/kg/day isoproterenol administration for 7 days, and the diltiazem group received 25 mg/kg/day diltiazem for 4 weeks. Following the treatment, paraffin sections were prepared to observe microstructural changes and to evaluate the concentration of Ca2+ in myocardium. The expression of transforming growth factors‑β1 (TGF‑β1), mothers against decapentaplegic homologues (Smad)2 and 7 and Cx43, were analyzed by reverse transcription-quantitative polymerase chain reaction and western blotting. The percentage Cx43 expression in intercalated disks was evaluated using immunohistochemistry. Fibrosis did not differ significantly between the control and the diltiazem‑treated group. The concentration of Ca2+ increased in the myocardium of model rats. The expression of Smad7 and Cx43 was decreased in the rat model, while the expression of TGF‑β1 and Smad2 was increased. There was a significant decrease in the relative abundance of intercalated disk Cx43 in the model group. The results of the present study suggest that diltiazem may serve a protective role during remodeling of myocardial ischemia, especially in fibrosis and Cx43 remodeling.
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Copy and paste a formatted citation
Spandidos Publications style
Wei Y, Meng T and Sun C: Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol. Mol Med Rep 17: 495-501, 2018.
APA
Wei, Y., Meng, T., & Sun, C. (2018). Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol. Molecular Medicine Reports, 17, 495-501. https://doi.org/10.3892/mmr.2017.7906
MLA
Wei, Y., Meng, T., Sun, C."Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol". Molecular Medicine Reports 17.1 (2018): 495-501.
Chicago
Wei, Y., Meng, T., Sun, C."Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol". Molecular Medicine Reports 17, no. 1 (2018): 495-501. https://doi.org/10.3892/mmr.2017.7906
Copy and paste a formatted citation
x
Spandidos Publications style
Wei Y, Meng T and Sun C: Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol. Mol Med Rep 17: 495-501, 2018.
APA
Wei, Y., Meng, T., & Sun, C. (2018). Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol. Molecular Medicine Reports, 17, 495-501. https://doi.org/10.3892/mmr.2017.7906
MLA
Wei, Y., Meng, T., Sun, C."Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol". Molecular Medicine Reports 17.1 (2018): 495-501.
Chicago
Wei, Y., Meng, T., Sun, C."Protective effect of diltiazem on myocardial ischemic rats induced by isoproterenol". Molecular Medicine Reports 17, no. 1 (2018): 495-501. https://doi.org/10.3892/mmr.2017.7906
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