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Article

Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells

  • Authors:
    • Jing Wang
    • Hui‑Min Gong
    • Hui‑Hui Zou
    • Ling Liang
    • Xin‑Yi Wu
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Qi Lu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China, Ophthalmic Center, Qingdao Municipal Hospital, Qingdao, Shandong 266000, P.R. China, Department of Ophthalmology, Dezhou People's Hospital, Dezhou, Shandong 253014, P.R. China
  • Pages: 648-652
    |
    Published online on: October 27, 2017
       https://doi.org/10.3892/mmr.2017.7916
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Abstract

Isorhamnetin, a 3‑O‑methylated metabolite of quercetin, exhibits antioxidant effects. However, to the best of our knowledge, no study to date has focused on the effects of isorhamnetin on retinal pigment epithelium (RPE) cells, and its underlying molecular mechanisms. Therefore, the present study aimed to examine the potential protective effect of isorhamnetin against oxidative stress in human RPE cells. The results demonstrated that pretreatment of RPE cells with isorhamnetin significantly protected cell viability against oxidative stress. In addition, isorhamnetin pretreatment inhibited hydrogen peroxide (H2O2)‑induced reactive oxygen species (ROS) production and caspase‑3 activation in RPE cells. Furthermore, isorhamnetin pretreatment significantly increased the phosphorylation of phosphoinositide 3‑kinase (PI3K) and AKT serine/threonine kinase 1 (Akt) in RPE cells exposed to H2O2, compared with cells treated with H2O2 alone. Taken together, the present results demonstrated that isorhamnetin protected human RPE cells from oxidative stress‑induced cell death, and this effect was associated with activation of the PI3K/Akt signaling pathway. Thus, isorhamnetin may be considered as a potential antioxidant useful for the prevention of age‑related macular degeneration.
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1 

Congdon NG, Friedman DS and Lietman T: Important causes of visual impairment in the world today. JAMA. 290:2057–2060. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Fletcher EC and Scholl HP: Ophthalmic disease in the ageing society. Ophthalmology and the Ageing Society. 1–9. 2013. View Article : Google Scholar

3 

Curcio CA, Zanzottera EC, Messinger JD, Ach T, Smith T and Freund KB: Retinal pigment epithelium (RPE) transdifferentiation and death in age-related macular degeneration (AMD), seen in the Project MACULA grading system. Invest Ophth Vis Sci. 56:893. 2015.

4 

Beatty S, Koh HH, Phil M, Henson D and Boulton M: The role of oxidative stress in the pathogenesis of age-related macular degeneration. Surv Ophthalmol. 45:115–134. 2000. View Article : Google Scholar : PubMed/NCBI

5 

Jarrett SG and Boulton ME: Consequences of oxidative stress in age-related macular degeneration. Mol Aspects Med. 33:399–417. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Liang FQ and Godley BF: Oxidative stress-induced mitochondrial DNA damage in human retinal pigment epithelial cells: A possible mechanism for RPE aging and age-related macular degeneration. Exp Eye Res. 76:397–403. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Drobek-Słowik M, Karczewicz D and Safranow K: The potential role of oxidative stress in the pathogenesis of the age-related macular degeneration (AMD). Postepy Hig Med Dosw (Online). 61:28–37. 2007.(In Polish). PubMed/NCBI

8 

Park JC, Young HS, Yu YB and Lee JH: Isorhamnetin sulphate from the leaves and stems of oenanthe javanica in korea. Planta Med. 61:377–378. 1995. View Article : Google Scholar : PubMed/NCBI

9 

Chen TL, Zhu GL, Wang JA, Zhang GD, Liu HF, Chen JR, Wang Y and He XL: Protective effects of isorhamnetin on apoptosis and inflammation in TNF-α-induced HUVECs injury. Int J Clin Exp Pathol. 8:2311–2320. 2015.PubMed/NCBI

10 

Saud SM, Young MR, Jones-Hall YL, Ileva L, Evbuomwan MO, Wise J, Colburn NH, Kim YS and Bobe G: Chemopreventive activity of plant flavonoid isorhamnetin in colorectal cancer is mediated by oncogenic Src and β-catenin. Cancer Res. 73:5473–5484. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Zhang N, Pei F, Wei H, Zhang T and Yang C, Ma G and Yang C: Isorhamnetin protects rat ventricular myocytes from ischemia and reperfusion injury. Exp Toxicol Pathol. 63:33–38. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Yang JH, Shin BY, Han JY, Kim MG, Wi JE, Kim YW, Cho IJ, Kim SC, Shin SM and Ki SH: Isorhamnetin protects against oxidative stress by activating Nrf2 and inducing the expression of its target genes. Toxicol Applied Pharmacol. 274:293–301. 2014. View Article : Google Scholar

13 

Kong CS, Kim JA, Qian ZJ, Kim YA, Lee JI, Kim SK, Nam TJ and Seo Y: Protective effect of isorhamnetin 3-О-β-d-glucopyranoside from Salicornia herbacea against oxidation-induced cell damage. Food Chem Toxicol. 47:1914–1920. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Bouhlel I, Skandrani I, Nefatti A, Valenti K, Ghedira K, Mariotte AM, Hininger-Favier I, Laporte F, Dijoux-Franca MG and Chekir-Ghedira L: Antigenotoxic and antioxidant activities of isorhamnetin 3-O neohesperidoside from Acacia salicina. Drug Chem Toxicol. 32:258–267. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Choi YH: The cytoprotective effect of isorhamnetin against oxidative stress is mediated by the upregulation of the Nrf2-dependent HO-1 expression in C2C12 myoblasts through scavenging reactive oxygen species and ERK inactivation. Gen Physiol Biophs. 35:145–154. 2016. View Article : Google Scholar

16 

Jänicke RU, Sprengart ML, Wati MR and Porter AG: Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis. J Biol Chem. 273:9357–9360. 1998. View Article : Google Scholar : PubMed/NCBI

17 

Faghiri Z and Bazan NG: PI3K/Akt and mTOR/p70S6K pathways mediate neuroprotection D1-induced retianl pigment epithelial cell survival during oxidarive stress-induced apoptosis. Exp Eye Res. 90:718–725. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Kim MH, Chung J, Yang JW, Chung SM, Kwag NH and Yoo JS: Hydrogen peroxide-induced cell death in a human retinal pigment epithelial cell line, ARPE-19. Korean J Ophthalmol. 17:19–28. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Hanneken A, Lin FF, Johnson J and Maher P: Flavonoids protect human retinal pigment epithelial cells from oxidative-stress-induced death. Invest Ophthalmol Vis Sci. 47:3164–3177. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Sreekumar PG, Kannan R, Yaung J, Spee CK, Ryan SJ and Hinton DR: Protection from oxidative stress by methionine sulfoxide reductases in RPE cells. Biochem Bioph Res Co. 334:245–253. 2005. View Article : Google Scholar

21 

Cai J, Nelson KC, Wu M, Sternberg P Jr and Jones DP: Oxidative damage and protection of the RPE. Prog Retin Eye Res. 19:205–221. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Sun B, Sun GB, Xiao J, Chen RC, Wang X, Wu Y, Cao L, Yang ZH and Sun XB: Isorhamnetin inhibits H2O2-induced activation of the intrinsic apoptotic pathway in H9c2 cardiomyocytes through scavenging reactive oxygen species and ERK inactivation. J Cell Biochem. 113:473–485. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Porter AG and Jänicke RU: Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 6:99–104. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Kook D, Wolf AH, Alice LY, Neubauer AS, Priglinger SG, Kampik A and Welge-Luüssen UC: The protective effect of quercetin against oxidative stress in the human RPE in vitro. Invest Ophth Vis Sci. 49:1712–1720. 2008. View Article : Google Scholar

25 

Wang R, Peng L, Zhao J, Zhang L, Guo C, Zheng W and Chen H: Gardenamide A protects RGC-5 cells from H2O2-induced oxidative stress insults by activating PI3K/Akt/eNOS signaling pathway. Int J Mol Sci. 16:22350–22367. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Zha X, Wu G, Zhao X, Zhou L, Zhang H, Li J, Ma L and Zhang Y: PRDX6 protects ARPE-19 cells from oxidative damage via PI3K/AKT signaling. Cell Physiol Biochem. 36:2217–2228. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Li Z, Dong X, Liu H, Chen X, Shi H, Fan Y, Hou D and Zhang X: Astaxanthin protects ARPE-19 cells from oxidative stress via upregulation of Nrf2-regulated phase II enzymes through activation of PI3K/Akt. Mol Vis. 19:1656–1666. 2013.PubMed/NCBI

28 

Yang P, Peairs JJ, Tano R and Jaffe GJ: Oxidant-mediated Akt activation in human RPE cells. Invest Ophthalmol Vis Sci. 47:4598–4606. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Luo Y, Sun G, Dong X, Wang M, Qin M, Yu Y and Sun X: Isorhamnetin attenuates atherosclerosis by inhibiting macrophage apoptosis via PI3K/AKT activation and HO-1 induction. PLoS One. 10:e01202592015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Gong HM, Zou HH, Liang L and Wu XY: Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells. Mol Med Rep 17: 648-652, 2018.
APA
Wang, J., Gong, H., Zou, H., Liang, L., & Wu, X. (2018). Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells. Molecular Medicine Reports, 17, 648-652. https://doi.org/10.3892/mmr.2017.7916
MLA
Wang, J., Gong, H., Zou, H., Liang, L., Wu, X."Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells". Molecular Medicine Reports 17.1 (2018): 648-652.
Chicago
Wang, J., Gong, H., Zou, H., Liang, L., Wu, X."Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells". Molecular Medicine Reports 17, no. 1 (2018): 648-652. https://doi.org/10.3892/mmr.2017.7916
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Gong HM, Zou HH, Liang L and Wu XY: Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells. Mol Med Rep 17: 648-652, 2018.
APA
Wang, J., Gong, H., Zou, H., Liang, L., & Wu, X. (2018). Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells. Molecular Medicine Reports, 17, 648-652. https://doi.org/10.3892/mmr.2017.7916
MLA
Wang, J., Gong, H., Zou, H., Liang, L., Wu, X."Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells". Molecular Medicine Reports 17.1 (2018): 648-652.
Chicago
Wang, J., Gong, H., Zou, H., Liang, L., Wu, X."Isorhamnetin prevents H2O2‑induced oxidative stress in human retinal pigment epithelial cells". Molecular Medicine Reports 17, no. 1 (2018): 648-652. https://doi.org/10.3892/mmr.2017.7916
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