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Article

Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells

  • Authors:
    • Deqiang Lei
    • Fangcheng Zhang
    • Dongxiao Yao
    • Nanxiang Xiong
    • Xiaobing Jiang
    • Hongyang Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 667-673
    |
    Published online on: October 27, 2017
       https://doi.org/10.3892/mmr.2017.7920
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Abstract

Galangin (3,5,7‑trihydroxyflavone), is a natural flavonoid present in plants. Galangin is reported to exhibit anti‑cancer properties against various cancer types. The aim of the present study was to display the effects of galangin on glioma and its mechanism of action in A172 human glioma cancer cells. The results clearly indicated that treatment of galangin inhibited A172 cell migration and invasion under non‑toxic doses. A human proteinase array assay was conducted to elucidate the potential effects of galangin, and the obtained results demonstrated that treatment of galangin inhibited ADAM9 protein expression and mRNA expression, that are known to contribute to cancer progression. Sustained extracellular signal‑regulated kinase (Erk)1/2 activation was also monitored, which contributed to ADAM9 protein expression and mRNA inhibition as investigated using western blotting analysis and reverse transcription‑quantitative polymerase chain reaction experiment. Erk1/2 inhibition by inhibitor or small interfering (si)Erk transfection markedly terminated galangin‑inhibited A172 migration and invasion via an Erk1/2 activation mechanism. Collective results suggested that galangin may act as an effective chemotherapeutic agent for glioma cancer depending on its ability to bring about ADAM9 and Erk1/2 activation.
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Copy and paste a formatted citation
Spandidos Publications style
Lei D, Zhang F, Yao D, Xiong N, Jiang X and Zhao H: Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells. Mol Med Rep 17: 667-673, 2018.
APA
Lei, D., Zhang, F., Yao, D., Xiong, N., Jiang, X., & Zhao, H. (2018). Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells. Molecular Medicine Reports, 17, 667-673. https://doi.org/10.3892/mmr.2017.7920
MLA
Lei, D., Zhang, F., Yao, D., Xiong, N., Jiang, X., Zhao, H."Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells". Molecular Medicine Reports 17.1 (2018): 667-673.
Chicago
Lei, D., Zhang, F., Yao, D., Xiong, N., Jiang, X., Zhao, H."Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells". Molecular Medicine Reports 17, no. 1 (2018): 667-673. https://doi.org/10.3892/mmr.2017.7920
Copy and paste a formatted citation
x
Spandidos Publications style
Lei D, Zhang F, Yao D, Xiong N, Jiang X and Zhao H: Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells. Mol Med Rep 17: 667-673, 2018.
APA
Lei, D., Zhang, F., Yao, D., Xiong, N., Jiang, X., & Zhao, H. (2018). Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells. Molecular Medicine Reports, 17, 667-673. https://doi.org/10.3892/mmr.2017.7920
MLA
Lei, D., Zhang, F., Yao, D., Xiong, N., Jiang, X., Zhao, H."Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells". Molecular Medicine Reports 17.1 (2018): 667-673.
Chicago
Lei, D., Zhang, F., Yao, D., Xiong, N., Jiang, X., Zhao, H."Galangin increases ERK1/2 phosphorylation to decrease ADAM9 expression and prevents invasion in A172 glioma cells". Molecular Medicine Reports 17, no. 1 (2018): 667-673. https://doi.org/10.3892/mmr.2017.7920
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