Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm

  • Authors:
    • Liang Wei
    • Qi Wang
    • Yanfei Zhang
    • Cheng Yang
    • Hongxin Guan
    • Yiming Chen
    • Zhiyang Sun
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 891-897
    |
    Published online on: November 3, 2017
       https://doi.org/10.3892/mmr.2017.7940
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Intracranial aneurysm (IA) is a devastating disease, the pathogenesis of which remains to be elucidated. The present study aimed to determine the molecular mechanism of IA and to identify potential therapeutic targets using bioinformatics analysis. The GSE54083 dataset, which includes data from patients with ruptured IA and superficial temporal artery controls, was downloaded from the Gene Expression Omnibus, and differentially expressed genes (DEGs) were identified in the ruptured IA samples using the limma package in R. Subsequently, the Database for Annotation, Visualization and Integrated Discovery software was used to perform function and pathway enrichment analyses and the Search Tool for the Retrieval of Interacting Genes database was used to construct the protein‑protein interaction (PPI) network. Then, microRNA (miRNA) target and transcription factor (TF) target pairs were identified using the miR2Disease, MiRwalk2, ITFP and TRANSFAC databases. Finally, an integrated network of TF‑target‑miRNAs was constructed using Cytoscape. A total of 402 upregulated DEGs and 375 downregulated DEGs were identified from the ruptured IA samples compared with the superficial temporal artery samples. The majority of the upregulated DEGs were significantly enriched in the immune system development category, including CD40 ligand (CD40LG) and CD40 and the downregulated DEGs, such as striatin (STRN), were enriched in neuron projection development. In addition, nitric oxide synthase 1 (NOS1), a target of miRNA‑125b, and myosin heavy chain 11 (MYH11), a target of minichromosome maintenance complex component 4 (MCM4), had higher degree scores in the integrated network. These findings suggest that CD40, CD40LG, NOS1, STRN, MCM4, MYH11 and miR‑125b may be potential therapeutic targets for the treatment of IA.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Chalouhi N, Hoh BL and Hasan D: Review of cerebral aneurysm formation, growth, and rupture. Stroke. 44:3613–3622. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Blignaut Gerrit, Loggenberg Eugene, Vries D and Coert: The radiological appearance of intracranial aneurysms in adults infected with the human immunodeficiency virus (HIV). Orig Res Sa J Radiol. 18:1–4. 2014.

3 

Brown RD Jr: Management of cerebral aneurysms. Mayo Clinic Proceedings. 79:288–302. 2004. View Article : Google Scholar

4 

Olafsson E, Hauser WA and Gudmundsson G: A population-based study of prognosis of ruptured cerebral aneurysm: Mortality and recurrence of subarachnoid hemorrhage. Neurology. 48:1191–1195. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Varble N and Meng H: Vortex imprints at the wall, but not in the bulk, distinguish ruptured from unruptured intracranial aneurysms. Presented at the DFD15 Meeting of The American Physical Society. (abstract L23.00008). 2015, http://meetings.aps.org/link/BAPS.2015.DFD.L23.8

6 

Lall RR, Eddleman CS, Bendok BR and Batjer HH: Unruptured intracranial aneurysms and the assessment of rupture risk based on anatomical and morphological factors: Sifting through the sands of data. Neurosurg Focus. 26:E22009. View Article : Google Scholar : PubMed/NCBI

7 

Mohan D, Munteanu V, Coman T and Ciurea AV: Genetic factors involves in intracranial aneurysms-actualities. J Med Life. 8:336–341. 2015.PubMed/NCBI

8 

Akagawa H, Tajima A, Sakamoto Y, Krischek B, Yoneyama T, Kasuya H, Onda H, Hori T, Kubota M, Machida T, et al: A haplotype spanning two genes, ELN and LIMK1, decreases their transcripts and confers susceptibility to intracranial aneurysms. Hum Mol Genet. 15:1722–1734. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Xu J, Ma F, Yan W, Qiao S, Xu S, Li Y, Luo J, Zhang J and Jin J: Identification of the soluble form of tyrosine kinase receptor Axl as a potential biomarker for intracranial aneurysm rupture. BMC Neurol. 15:232015. View Article : Google Scholar : PubMed/NCBI

10 

Ollikainen E, Tulamo R, Lehti S, Lee-Rueckert M, Hernesniemi J, Niemelä M, Ylä-Herttuala S, Kovanen PT and Frösen J: Smooth muscle cell foam cell formation, apolipoproteins and ABCA1 in intracranial aneurysms: Implications for lipid accumulation as a promoter of aneurysm wall rupture. J Neuropathol Exp Neurol. 75:689–699. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Luo J, Jin H, Jiang Y, Ge H, Wang J and Li Y: Aberrant Expression of microRNA-9 contributes to development of intracranial aneurysm by suppressing proliferation and reducing contractility of smooth muscle cells. Med Sci Monit. 22:4247–4253. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Wu X, Zhang J, Huang Q, Yang P, Chen J and Liu J: MicroRNA-92a regulates expression of kruppel-like factor2 in rabbit model of intracranial aneurysm. Cell Mol Biol (Noisy-le-grand). 61:44–48. 2015.PubMed/NCBI

13 

Aoki T, Kataoka H, Nishimura M, Ishibashi R, Morishita R and Miyamoto S: Ets-1 promotes the progression of cerebral aneurysm by inducing the expression of MCP-1 in vascular smooth muscle cells. Gene Ther. 17:1117–1123. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Nakaoka H, Tajima A, Yoneyama T, Hosomichi K, Kasuya H, Mizutani T and Inoue I: Gene expression profiling reveals distinct molecular signatures associated with the rupture of intracranial aneurysm. Stroke. 45:2239–2245. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Irizarry RA, Hobbs B, Collin F, Beazer-Barclay YD, Antonellis KJ, Scherf U and Speed TP: Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 4:249–264. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Li C, Wei Z and Li H: Network-based empirical bayes methods for linear models with applications to genomic data. J Biopharm Stat. 20:209–222. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Benjamini Y and Hochberg Y: Controlling the false discovery rate: A practical and powerful approach to multiple testing. J R Statist Soc B. 57:289–300. 1995.

18 

da Huang W, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Franceschini A, Szklarczyk D, Frankild S, Kuhn M, Simonovic M, Roth A, Lin J, Minguez P, Bork P, von Mering C and Jensen LJ: STRING v9.1: Protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res. 41:(Database issue). D808–D815. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Tang Y, Li M, Wang J, Pan Y and Wu FX: CytoNCA: A cytoscape plugin for centrality analysis and evaluation of protein interaction networks. Bio Systems. 127:67–72. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Jiang Q, Wang Y, Hao Y, Juan L, Teng M, Zhang X, Li M, Wang G and Liu Y: miR2Disease: A manually curated database for microRNA deregulation in human disease. Nucleic Acids Res. 37:(Database issue). D98–D104. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Chalouhi N, Ali MS, Jabbour PM, Tjoumakaris SI, Gonzalez LF, Rosenwasser RH, Koch WJ and Dumont AS: Biology of intracranial aneurysms: Role of inflammation. J Cereb Blood Flow Metab. 32:1659–1676. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Aoki T, Fukuda M and Narumiya S: Chronic inflammation in intracranial aneurysm formation. Inflamm Regen. 33:283–287. 2013. View Article : Google Scholar

24 

Tulamo R, Frösen J, Junnikkala S, Paetau A, Pitkäniemi J, Kangasniemi M, Niemelä M, Jääskeläinen J, Jokitalo E, Karatas A, et al: Complement activation associates with saccular cerebral artery aneurysm wall degeneration and rupture. Neurosurgery. 59:1076–1077. 2006. View Article : Google Scholar

25 

Kataoka K, Taneda M, Asai T, Kinoshita A, Ito M and Kuroda R: Structural fragility and inflammatory response of ruptured cerebral aneurysms. A comparative study between ruptured and unruptured cerebral aneurysms. Stroke. 30:1396–1401. 1999. View Article : Google Scholar : PubMed/NCBI

26 

Pyysalo MJ, Pyysalo LM, Pessi T, Karhunen PJ and Öhman JE: The connection between ruptured cerebral aneurysms and odontogenic bacteria. Öhman JE. 84:1214–1218. 2013.

27 

Nagashima H, Aoka Y, Sakomura Y, Uto K, Sakuta A, Aomi S, Kurosawa H, Hagiwara N, Kawana M and Kasanuki H: Matrix metalloproteinase 2 is suppressed by trapidil, a CD40-CD40 ligand pathway inhibitor, in human abdominal aortic aneurysm wall. J Vasc Surg. 39:447–453. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Ochs HD, Hollenbaugh D and Aruffo A: The role of CD40L (gp39)/CD40 in T/B cell interaction and primary immunodeficiency. Semin Immunol. 6:337–341. 1994. View Article : Google Scholar : PubMed/NCBI

29 

Bartoli M, Ternaux JP, Forni C, Portalier P, Salin P, Amalric M and Monneron A: Down-regulation of striatin, a neuronal calmodulin-binding protein, impairs rat locomotor activity. J Neurobiol. 40:234–243. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Liu D, Han L, Wu X, Yang X, Zhang Q and Jiang F: Genome-wide microRNA changes in human intracranial aneurysms. BMC Neurol. 14:1882014. View Article : Google Scholar : PubMed/NCBI

31 

Boyle JJ, Weissberg PL and Bennett MR: Human macrophage-induced vascular smooth muscle cell apoptosis requires NO enhancement of Fas/Fas-L interactions. Arterioscler Thromb Vasc Biol. 22:1624–1630. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Li J, Feng W, Chen L and He J: Downregulation of SMC1A inhibits growth and increases apoptosis and chemosensitivity of colorectal cancer cells. J Int Med Res. 44:67–74. 2016. View Article : Google Scholar : PubMed/NCBI

33 

Hughes CR, Guasti L, Meimaridou E, Chuang CH, Schimenti JC, King PJ, Costigan C, Clark AJ and Metherell LA: MCM4 mutation causes adrenal failure, short stature, and natural killer cell deficiency in humans. J Clin Invest. 122:814–820. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Hinterseher I, Schworer CM, Lillvis JH, Stahl E, Erdman R, Gatalica Z, Tromp G and Kuivaniemi H: Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms. Int J Mol Sci. 16:11196–11212. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Ravindra VM, Karsy M, Schmidt RH, Taussky P, Park MS and Bollo RJ: Rapid de novo aneurysm formation after clipping of a ruptured middle cerebral artery aneurysm in an infant with an MYH11 mutation. J Neurosurg Pediatr. 18:463–470. 2016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wei L, Wang Q, Zhang Y, Yang C, Guan H, Chen Y and Sun Z: Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm. Mol Med Rep 17: 891-897, 2018.
APA
Wei, L., Wang, Q., Zhang, Y., Yang, C., Guan, H., Chen, Y., & Sun, Z. (2018). Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm. Molecular Medicine Reports, 17, 891-897. https://doi.org/10.3892/mmr.2017.7940
MLA
Wei, L., Wang, Q., Zhang, Y., Yang, C., Guan, H., Chen, Y., Sun, Z."Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm". Molecular Medicine Reports 17.1 (2018): 891-897.
Chicago
Wei, L., Wang, Q., Zhang, Y., Yang, C., Guan, H., Chen, Y., Sun, Z."Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm". Molecular Medicine Reports 17, no. 1 (2018): 891-897. https://doi.org/10.3892/mmr.2017.7940
Copy and paste a formatted citation
x
Spandidos Publications style
Wei L, Wang Q, Zhang Y, Yang C, Guan H, Chen Y and Sun Z: Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm. Mol Med Rep 17: 891-897, 2018.
APA
Wei, L., Wang, Q., Zhang, Y., Yang, C., Guan, H., Chen, Y., & Sun, Z. (2018). Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm. Molecular Medicine Reports, 17, 891-897. https://doi.org/10.3892/mmr.2017.7940
MLA
Wei, L., Wang, Q., Zhang, Y., Yang, C., Guan, H., Chen, Y., Sun, Z."Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm". Molecular Medicine Reports 17.1 (2018): 891-897.
Chicago
Wei, L., Wang, Q., Zhang, Y., Yang, C., Guan, H., Chen, Y., Sun, Z."Identification of key genes, transcription factors and microRNAs involved in intracranial aneurysm". Molecular Medicine Reports 17, no. 1 (2018): 891-897. https://doi.org/10.3892/mmr.2017.7940
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team