Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain

  • Authors:
    • Guanghui Jin
    • Xing Jin
    • Shuang Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Affiliated Hospital of Beihua University, Jilin, Jilin 132000, P.R. China
  • Pages: 1247-1252
    |
    Published online on: November 3, 2017
       https://doi.org/10.3892/mmr.2017.7951
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

It has been previously demonstrated that sparstolonin B (SsnB) inhibits toll‑like receptor (TLR)‑2 and TLR‑4. The present study investigated the effect of SsnB on neuropathic pain (NP). A chronic constriction injury (CCI) model was constructed in rats and the protein expression of TLR‑2 and TLR‑4 was determined by western blot analysis. Rats were divided into the following three groups: Rats with sham surgery (control group); rats with CCI (model group); and rats with CCI and injection of SsnB (SsnB group). The mechanical withdrawal threshold (MWT) was measured by using Von Frey filaments. In addition, the mRNA and protein expression levels of nuclear factor‑κB (NF‑κB) were investigated by reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively, and the concentrations of tumor necrosis factor‑α (TNF‑α) and interleukin (IL)‑6 were determined by ELISA. Compared with control rats, the protein expression levels of TLR‑2 and TLR‑4 were increased in model rats (P<0.001). At 7 and 14 days after surgery, the MWTs in the model group were significantly reduced compared with the control group (P<0.001). However, the MWTs in the SsnB group were significantly increased compared with the model group (P<0.001). The results also demonstrated that the mRNA and protein expression levels of NF‑κB, and the protein expression levels of TNF‑α and IL‑6, were increased in model group compared with the control group (P<0.001). Furthermore, these increases in expression were all reduced in the SsnB group compared with the model group. Therefore, the results indicate that SsnB may alleviate NP via suppression of TLR‑2 and TLR‑4, and may be a potential drug for the treatment of NP.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Jaggi AS and Singh N: Therapeutic targets for the management of peripheral nerve injury-induced neuropathic pain. CNS Neurol Disord Drug Targets. 10:589–609. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Mukherjee P, Cinelli MA, Kang S and Silverman RB: Development of nitric oxide synthase inhibitors for neurodegeneration and neuropathic pain. Chem Soc Rev. 43:6814–6838. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Sisignano M, Baron R, Scholich K and Geisslinger G: Mechanism-based treatment for chemotherapy-induced peripheral neuropathic pain. Nat Rev Neurol. 10:694–707. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Attal N, Cruccu G, Baron R, Haanpää M, Hansson P, Jensen TS and Nurmikko T: European Federation of Neurological Societies: EFNS guidelines on the pharmacological treatment of neuropathic pain: 2010 revision. Eur J Neurol. 17:1113–1188. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Costigan M, Scholz J and Woolf CJ: Neuropathic pain: A maladaptive response of the nervous system to damage. Annu Rev Neurosci. 32:1–32. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Bouhassira D, Lantéri-Minet M, Attal N, Laurent B and Touboul C: Prevalence of chronic pain with neuropathic characteristics in the general population. Pain. 136:380–387. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Gilron I, Baron R and Jensen T: Neuropathic Pain: Principles of diagnosis and treatment. Mayo Clinic Proc. 90:532–545. 2015. View Article : Google Scholar

8 

Rajamani U and Jialal I: Hyperglycemia induces Toll-like receptor-2 and −4 expression and activity in human microvascular retinal endothelial cells: Implications for diabetic retinopathy. J Diabetes Res. 2014:7909022014. View Article : Google Scholar : PubMed/NCBI

9 

Madhumitha H, Mohan V, Kumar NP, Pradeepa R, Babu S and Aravindhan V: Impaired toll-like receptor signalling in peripheral B cells from newly diagnosed type-2 diabetic subjects. Cytokine. 76:253–259. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Xu Y, Jiang Z, Huang J, Meng Q, Coh P and Tao L: The association between toll-like receptor 4 polymorphisms and diabetic retinopathy in Chinese patients with type 2 diabetes. Br J Ophthalmol. 99:1301–1305. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Nicotra L, Loram LC, Watkins LR and Hutchinson MR: Toll-like receptors in chronic pain. Exp Neurol. 234:316–329. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Zhao M, Li CH and Liu YL: Toll-like receptor (TLR)-2/4 expression in retinal ganglion cells in a high-glucose environment and its implications. Genet Mol Res. 15:2016.

13 

Kim D, Kim MA, Cho IH, Kim MS, Lee S, Jo EK, Choi SY, Park K, Kim JS, Akira S, et al: A critical role of toll-like receptor 2 in nerve injury-induced spinal cord glial cell activation and pain hypersensitivity. J Biol Chem. 282:14975–14983. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Bettoni I, Comelli F, Rossini C, Granucci F, Giagnoni G, Peri F and Costa B: Glial TLR4 receptor as new target to treat neuropathic pain: Efficacy of a new receptor antagonist in a model of peripheral nerve injury in mice. Glia. 56:1312–1319. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Liang Q, Wu Q, Jiang J, Duan J, Wang C, Smith MD, Lu H, Wang Q, Nagarkatti P and Fan D: Characterization of sparstolonin B, a Chinese herb-derived compound, as a selective Toll-like receptor antagonist with potent anti-inflammatory properties. J Biol Chem. 286:26470–26479. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Liu Q, Li J, Liang Q, Wang D, Luo Y, Yu F, Janicki JS and Fan D: Sparstolonin B suppresses rat vascular smooth muscle cell proliferation, migration, inflammatory response and lipid accumulation. Vascul Pharmacol. 67–69:59–66. 2015. View Article : Google Scholar

17 

Wang M, Xiu L, Diao J, Wei L and Sun J: Sparstolonin B inhibits lipopolysaccharide-induced inflammation in 3T3-L1 adipocytes. Eur J Pharmacol. 769:79–85. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Liu Q, Wang J, Liang Q, Wang D, Luo Y, Li J, Janicki JS and Fan D: Sparstolonin B attenuates hypoxia-reoxygenation-induced cardiomyocyte inflammation. Exp Biol Med (Maywood). 239:376–384. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Zimmermann M: Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 16:109–110. 1983. View Article : Google Scholar : PubMed/NCBI

20 

Liu S, Zhu B, Sun Y and Xie X: MiR-155 modulates the progression of neuropathic pain through targeting SGK3. Int J Clin Exp Pathol. 8:14374–14382. 2015.PubMed/NCBI

21 

Zhou Z, Liang Y, Deng F, Cheng Y, Sun J, Guo L and Xu G: Phosphorylated neuronal nitric oxide synthase in neuropathic pain in rats. Int J Clin Exp Pathol. 8:12748–12756. 2015.PubMed/NCBI

22 

Zhang J, Zou N, Liang Q, Tang Y and Duan JA: Simultaneous HPLC quantitative analysis of nine bioactive constituents in Scirpus Yagara Ohwi (Cyperaceae). J Chromatogr Sci. 54:453–459. 2016.PubMed/NCBI

23 

Schefe JH, Lehmann KE, Buschmann IR, Unger T and Funke-Kaiser H: Quantitative real-time RT-PCR data analysis: Current concepts and the novel ‘gene expression's CT difference’ formula. J Mol Med (Berl). 84:901–910. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Dworkin RH, Turk DC, Katz NP, Rowbotham MC, Peirce-Sandner S, Cerny I, Clingman CS, Eloff BC, Farrar JT, Kamp C, et al: Evidence-based clinical trial design for chronic pain pharmacotherapy: A blueprint for ACTION. Pain. 152 Suppl 3:S107–S115. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Kawasaki Y, Zhang L, Cheng JK and Ji RR: Cytokine mechanisms of central sensitization: Distinct and overlapping role of interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha in regulating synaptic and neuronal activity in the superficial spinal cord. J Neurosci. 28:5189–5194. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Manning DC: New and emerging pharmacological targets for neuropathic pain. Curr Pain Headache Rep. 8:192–198. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Smith JA, Das A, Ray SK and Banik NL: Role of pro-inflammatory cytokines released from microglia in neurodegenerative diseases. Brain Res Bull. 87:10–20. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Shi XQ, Zekki H and Zhang J: The role of TLR2 in nerve injury-induced neuropathic pain is essentially mediated through macrophages in peripheral inflammatory response. Glia. 59:231–241. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Wu FX, Bian JJ, Miao XR, Huang SD, Xu XW, Gong DJ, Sun YM, Lu ZJ and Yu WF: Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain. Int J Med Sci. 7:251–259. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Jurga AM, Rojewska E, Piotrowska A, Makuch W, Pilat D, Przewlocka B and Mika J: Blockade of toll-like receptors (TLR2, TLR4) attenuates pain and potentiates buprenorphine analgesia in a rat neuropathic pain model. Neural Plast. 2016:52387302016. View Article : Google Scholar : PubMed/NCBI

31 

Zhang Y, Zhang Y, Miao J, Hanley G, Stuart C, Sun X, Chen T and Yin D: Chronic restraint stress promotes immune suppression through toll-like receptor 4-mediated phosphoinositide 3-kinase signaling. J Neuroimmunol. 204:13–19. 2008. View Article : Google Scholar : PubMed/NCBI

32 

Blich M, Golan A, Arvatz G, Sebbag A, Shafat I, Sabo E, Cohen-Kaplan V, Petcherski S, Avniel-Polak S, Eitan A, et al: Macrophages activation by heparanase is mediated by TLR-2 and TLR-4 and associates with plaque progression. Arterioscler Thromb Vasc Biol. 33:e56–e65. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Verstrepen L, Bekaert T, Chau TL, Tavernier J, Chariot A and Beyaert R: TLR-4, IL-1R and TNF-R signaling to NF-kappaB: Variations on a common theme. Cell Mol Life Sci. 65:2964–2978. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Palová-Jelínková L, Dáňová K, Drašarová H, Dvořák M, Funda DP, Fundová P, Kotrbová-Kozak A, Černá M, Kamanová J, Martin SF, et al: Pepsin digest of wheat gliadin fraction increases production of IL-1β via TLR4/MyD88/TRIF/MAPK/NF-κB signaling pathway and an NLRP3 inflammasome activation. PLoS One. 8:e624262013. View Article : Google Scholar : PubMed/NCBI

35 

Zhang Q, Raoof M, Chen Y, Sumi Y, Sursal T, Junger W, Brohi K, Itagaki K and Hauser CJ: Circulating mitochondrial DAMPs cause inflammatory responses to injury. Nature. 464:104–107. 2010. View Article : Google Scholar : PubMed/NCBI

36 

Cao H and Zhang YQ: Spinal glial activation contributes to pathological pain states. Neurosci Biobehav Rev. 32:972–983. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Jin G, Jin X and Zhou S: Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain. Mol Med Rep 17: 1247-1252, 2018.
APA
Jin, G., Jin, X., & Zhou, S. (2018). Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain. Molecular Medicine Reports, 17, 1247-1252. https://doi.org/10.3892/mmr.2017.7951
MLA
Jin, G., Jin, X., Zhou, S."Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain". Molecular Medicine Reports 17.1 (2018): 1247-1252.
Chicago
Jin, G., Jin, X., Zhou, S."Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain". Molecular Medicine Reports 17, no. 1 (2018): 1247-1252. https://doi.org/10.3892/mmr.2017.7951
Copy and paste a formatted citation
x
Spandidos Publications style
Jin G, Jin X and Zhou S: Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain. Mol Med Rep 17: 1247-1252, 2018.
APA
Jin, G., Jin, X., & Zhou, S. (2018). Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain. Molecular Medicine Reports, 17, 1247-1252. https://doi.org/10.3892/mmr.2017.7951
MLA
Jin, G., Jin, X., Zhou, S."Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain". Molecular Medicine Reports 17.1 (2018): 1247-1252.
Chicago
Jin, G., Jin, X., Zhou, S."Sparstolonin B selectively suppresses toll‑like receptor‑2 and ‑4 to alleviate neuropathic pain". Molecular Medicine Reports 17, no. 1 (2018): 1247-1252. https://doi.org/10.3892/mmr.2017.7951
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team