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Article

LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia

  • Authors:
    • Chunhong Wang
    • Chunfang Han
    • Yibo Zhang
    • Fengqin Liu
  • View Affiliations / Copyright

    Affiliations: The Third Department of Geriatrics, Weifang People's Hospital, Weifang, Shandong 261014, P.R. China, Department of Pediatrics, Weifang People's Hospital, Weifang, Shandong 261014, P.R. China
  • Pages: 1105-1110
    |
    Published online on: November 3, 2017
       https://doi.org/10.3892/mmr.2017.7962
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Abstract

Non‑small cell lung cancer (NSCLC) represents one of the most important causes of cancer mortality in the world, and leads to the largest number of deaths in all kinds of lung cancer. Hypoxia has been confirmed to be a characteristic feature of NSCLC and has been shown to decrease the therapeutic efficacy of radiotherapy and some forms of chemotherapy. Previous studies revealed that many miRNAs have been proven to be involved in the molecular regulation of hypoxia and to affect the protein expression level of HIF‑1α. Here, we demonstrated that miR‑199a‑5p downregulated HIF‑1α expression and was involved in regulating the proliferation of NLSCS cell under hypoxia through downregulation of HIF‑1α. Recently, PVT1 has been proposed to function as a molecular sponge by competitively binding miR‑199a‑5p using miRcode. In this study, we confirmed that PVT1 was overexpressed in the hypoxic lung cancer cells, and then we further demonstrated that PVT1 functioned as competing endogenous (ce)RNA for miR‑199a‑5p, upregulated expression of its endogenous targets HIF‑1α and inhibited its function. Collectively, our study suggested that PVT1 promotes expression of HIF‑1α in NSCLC by functioning as ceRNA of miR‑199a‑5p. These findings support the hypothesis that PVT1 is a vital potential target for hypoxia therapy.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Miller YE: Pathogenesis of lung cancer: 100 year report. Am J Respir Cell Mol Biol. 33:216–223. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Semenza GL: Targeting HIF-1 for cancer therapy. Nat Rev Cancer. 3:721–732. 2003. View Article : Google Scholar : PubMed/NCBI

4 

Adams JM, Difazio LT, Rolandelli RH, Luján JJ, Haskó G, Csóka B, Selmeczy Z and Németh ZH: HIF-1: A key mediator in hypoxia. Acta Physiol Hung. 96:19–28. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Takasaki C, Kobayashi M, Ishibashi H, Akashi T and Okubo K: Expression of hypoxia-inducible factor-1α affects tumor proliferation and antiapoptosis in surgically resected lung cancer. Mol Clin Oncol. 5:295–300. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Yang SL, Ren QG, Wen L and Hu JL: Clinicopathological and prognostic significance of hypoxia-inducible factor-1 alpha in lung cancer: A systematic review with meta-analysis. J Huazhong Univ Sci Technolog Med Sci. 36:321–327. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Ren W, Mi D, Yang K, Cao N, Tian J, Li Z and Ma B: The expression of hypoxia-inducible factor-1α and its clinical significance in lung cancer: A systematic review and meta-analysis. Swiss Med Wkly. 143:w138552013.PubMed/NCBI

8 

Cha ST, Chen PS, Johansson G, Chu CY, Wang MY, Jeng YM, Yu SL, Chen JS, Chang KJ, Jee SH, et al: MicroRNA-519c suppresses hypoxia-inducible factor-1alpha expression and tumor angiogenesis. Cancer Res. 70:2675–2685. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Ding G, Huang G, Liu HD, Liang HX, Ni YF, Ding ZH, Ni GY and Hua HW: MiR-199a suppresses the hypoxia-induced proliferation of non-small cell lung cancer cells through targeting HIF1α. Mol Cell Biochem. 384:173–180. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Wei X, Wang C, Ma C, Sun W, Li H and Cai Z: Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1α and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia. Cancer Cell Int. 16:732016. View Article : Google Scholar : PubMed/NCBI

11 

Huang C, Liu S, Wang H, Zhang Z, Yang Q and Gao F: LncRNA PVT1 overexpression is a poor prognostic biomarker and regulates migration and invasion in small cell lung cancer. Am J Transl Res. 8:5025–5034. 2016.PubMed/NCBI

12 

Wan L, Sun M, Liu GJ, Wei CC, Zhang EB, Kong R, Xu TP, Huang MD and Wang ZX: Long noncoding RNA PVT1 promotes non-small cell lung cancer cell proliferation through epigenetically regulating LATS2 expression. Mol Cancer Ther. 15:1082–1094. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Cui D, Yu CH, Liu M, Xia QQ, Zhang YF and Jiang WL: Long non-coding RNA PVT1 as a novel biomarker for diagnosis and prognosis of non-small cell lung cancer. Tumour Biol. 37:4127–4134. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Conte F, Fiscon G, Chiara M, Colombo T, Farina L and Paci P: Role of the long non-coding RNA PVT1 in the dysregulation of the ceRNA-ceRNA network in human breast cancer. PLoS One. 12:e01716612017. View Article : Google Scholar : PubMed/NCBI

15 

Harris AL: Hypoxia-a key regulatory factor in tumour growth. Nat Rev Cancer. 2:38–47. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Guerin E, Raffelsberger W, Pencreach E, Maier A, Neuville A, Schneider A, Bachellier P, Rohr S, Petitprez A, Poch O, et al: In vivo topoisomerase I inhibition attenuates the expression of hypoxia-inducible factor 1α target genes and decreases tumor angiogenesis. Mol Med. 18:83–94. 2012. View Article : Google Scholar : PubMed/NCBI

17 

Li L, Lin X, Shoemaker AR, Albert DH, Fesik SW and Shen Y: Hypoxia-inducible factor-1 inhibition in combination with temozolomide treatment exhibits robust antitumor efficacy in vivo. Clin Cancer Res. 12:4747–4754. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Tang CM and Yu J: Hypoxia-inducible factor-1 as a therapeutic target in cancer. J Gastroenterol Hepatol. 28:401–405. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Rane S, He M, Sayed D, Vashistha H, Malhotra A, Sadoshima J, Vatner DE, Vatner SF and Abdellatif M: Downregulation of miR-199a derepresses hypoxia-inducible factor-1alpha and Sirtuin 1 and recapitulates hypoxia preconditioning in cardiac myocytes. Circ Res. 104:879–886. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Cui M, Xiao Z, Wang Y, Zheng M, Song T, Cai X, Sun B, Ye L and Zhang X: Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway. Cancer Res. 75:846–857. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Quagliata L, Matter MS, Piscuoglio S, Arabi L, Ruiz C, Procino A, Kovac M, Moretti F, Makowska Z, Boldanova T, et al: Long noncoding RNA HOTTIP/HOXA13 expression is associated with disease progression and predicts outcome in hepatocellular carcinoma patients. Hepatology. 59:911–923. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Takahashi Y, Sawada G, Kurashige J, Uchi R, Matsumura T, Ueo H, Takano Y, Eguchi H, Sudo T, Sugimachi K, et al: Amplification of PVT-1 is involved in poor prognosis via apoptosis inhibition in colorectal cancers. Br J Cancer. 110:164–171. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Guan Y, Kuo WL, Stilwell JL, Takano H, Lapuk AV, Fridlyand J, Mao JH, Yu M, Miller MA, Santos JL, et al: Amplification of PVT1 contributes to the pathophysiology of ovarian and breast cancer. Clin Cancer Res. 13:5745–5755. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Grampp S, Platt JL, Lauer V, Salama R, Kranz F, Neumann VK, Wach S, Stöhr C, Hartmann A, Eckardt KU, et al: Genetic variation at the 8q24.21 renal cancer susceptibility locus affects HIF binding to a MYC enhancer. Nat Commun. 7:131832016. View Article : Google Scholar : PubMed/NCBI

25 

Salmena L, Poliseno L, Tay Y, Kats L and Pandolfi PP: A ceRNA hypothesis: The Rosetta Stone of a hidden RNA language? Cell. 146:353–358. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Sumazin P, Yang X, Chiu HS, Chung WJ, Iyer A, Llobet-Navas D, Rajbhandari P, Bansal M, Guarnieri P, Silva J and Califano A: An extensive microRNA-mediated network of RNA-RNA interactions regulates established oncogenic pathways in glioblastoma. Cell. 147:370–381. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Karreth FA, Tay Y, Perna D, Ala U, Tan SM, Rust AG, DeNicola G, Webster KA, Weiss D, Perez-Mancera PA, et al: In vivo identification of tumor-suppressive PTEN ceRNAs in an oncogenic BRAF-induced mouse model of melanoma. Cell. 147:382–395. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Hsu SD, Tseng YT, Shrestha S, Lin YL, Khaleel A, Chou CH, Chu CF, Huang HY, Lin CM, Ho SY, et al: miRTarBase update 2014: An information resource for experimentally validated miRNA-target interactions. Nucleic Acids Res. 42:(Database Issue). D78–D85. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Han C, Zhang Y and Liu F: LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia. Mol Med Rep 17: 1105-1110, 2018.
APA
Wang, C., Han, C., Zhang, Y., & Liu, F. (2018). LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia. Molecular Medicine Reports, 17, 1105-1110. https://doi.org/10.3892/mmr.2017.7962
MLA
Wang, C., Han, C., Zhang, Y., Liu, F."LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia". Molecular Medicine Reports 17.1 (2018): 1105-1110.
Chicago
Wang, C., Han, C., Zhang, Y., Liu, F."LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia". Molecular Medicine Reports 17, no. 1 (2018): 1105-1110. https://doi.org/10.3892/mmr.2017.7962
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Han C, Zhang Y and Liu F: LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia. Mol Med Rep 17: 1105-1110, 2018.
APA
Wang, C., Han, C., Zhang, Y., & Liu, F. (2018). LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia. Molecular Medicine Reports, 17, 1105-1110. https://doi.org/10.3892/mmr.2017.7962
MLA
Wang, C., Han, C., Zhang, Y., Liu, F."LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia". Molecular Medicine Reports 17.1 (2018): 1105-1110.
Chicago
Wang, C., Han, C., Zhang, Y., Liu, F."LncRNA PVT1 regulate expression of HIF1α via functioning as ceRNA for miR‑199a‑5p in non‑small cell lung cancer under hypoxia". Molecular Medicine Reports 17, no. 1 (2018): 1105-1110. https://doi.org/10.3892/mmr.2017.7962
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