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Article

Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts

  • Authors:
    • Changyao Wang
    • Cailong Zhang
    • Feng Zhou
    • Lei Gao
    • Yingzhen Wang
    • Chunsheng Wang
    • Yongtao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266061, P.R. China, Department of Orthopaedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 1166-1172
    |
    Published online on: November 6, 2017
       https://doi.org/10.3892/mmr.2017.7971
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Abstract

The present study investigated the effect of angiotensin II (Ang II) on monocyte chemoattractant protein‑1 (MCP‑1) expression and the underlying mechanism in osteoblasts. MCP‑1 expression levels were analyzed by ELISA and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Ang II type 1 receptor (AT1R) expression levels was examined by RT‑qPCR, western blotting and immunostaining. In addition, the nuclear factor (NF)‑κB signaling pathway was investigated via western blot analysis. Reactive oxygen species (ROS) were also detected by flow cytometry and fluorescent microscopy. The results of the present study ndicated that Ang II upregulated MCP‑1 expression in osteoblasts, which was mitigated by agonists of the AT1R, including olmesartan, a ROS scavenger N‑acetylcysteine (NAC), ammonium pyrrolidinethiocarbamate (PDTC) and nuclear factor (NF)‑κB, but not by the Ang II type 2 receptor antagonist, PD123319. Furthermore, Ang II increased the generation of ROS and activated the NF‑κB signaling pathway. These effects of Ang II were blocked by olmesartan, NAC and PDTC, but not by PD1123319 in osteoblasts. In conclusion, these data indicated that Ang II enhanced ROS production and activated NF‑κB signaling via AT1R, thus upregulating MCP‑1 expression in osteoblasts.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Zhang C, Zhou F, Gao L, Wang Y, Wang C and Zhang Y: Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Mol Med Rep 17: 1166-1172, 2018.
APA
Wang, C., Zhang, C., Zhou, F., Gao, L., Wang, Y., Wang, C., & Zhang, Y. (2018). Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Molecular Medicine Reports, 17, 1166-1172. https://doi.org/10.3892/mmr.2017.7971
MLA
Wang, C., Zhang, C., Zhou, F., Gao, L., Wang, Y., Wang, C., Zhang, Y."Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts". Molecular Medicine Reports 17.1 (2018): 1166-1172.
Chicago
Wang, C., Zhang, C., Zhou, F., Gao, L., Wang, Y., Wang, C., Zhang, Y."Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts". Molecular Medicine Reports 17, no. 1 (2018): 1166-1172. https://doi.org/10.3892/mmr.2017.7971
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Zhang C, Zhou F, Gao L, Wang Y, Wang C and Zhang Y: Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Mol Med Rep 17: 1166-1172, 2018.
APA
Wang, C., Zhang, C., Zhou, F., Gao, L., Wang, Y., Wang, C., & Zhang, Y. (2018). Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts. Molecular Medicine Reports, 17, 1166-1172. https://doi.org/10.3892/mmr.2017.7971
MLA
Wang, C., Zhang, C., Zhou, F., Gao, L., Wang, Y., Wang, C., Zhang, Y."Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts". Molecular Medicine Reports 17.1 (2018): 1166-1172.
Chicago
Wang, C., Zhang, C., Zhou, F., Gao, L., Wang, Y., Wang, C., Zhang, Y."Angiotensin II induces monocyte chemoattractant protein‑1 expression by increasing reactive oxygen species‑mediated activation of the nuclear factor‑κB signaling pathway in osteoblasts". Molecular Medicine Reports 17, no. 1 (2018): 1166-1172. https://doi.org/10.3892/mmr.2017.7971
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