Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress

  • Authors:
    • Bing Xiao
    • Jian‑Gang Wang
    • Fang Han
    • Yu‑Xiu Shi
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Basic Medical Sciences College, China Medical University, Shenyang, Liaoning 110122, P.R. China
  • Pages: 1099-1104
    |
    Published online on: November 6, 2017
       https://doi.org/10.3892/mmr.2017.7976
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The purpose of the present study was to investigate the role of endoplasmic reticulum (ER)‑resident molecular chaperone proteins to identify whether these proteins were involved in post‑traumatic stress disorder (PTSD). The present study detected changes of calreticulin (CRT), calnexin (CNX) and ERp57 in the amygdala of rats, which may with aim of providing a novel insight into the modulation effect of amygdala in PTSD. Single‑prolonged stress (SPS) was applied to create the models of PTSD in rats. The expression levels of CRT, CNX and ERp57 were examined using immunohistochemistry or immunofluorescence, western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The results showed that SPS induced significant changes in CRT, CNX and ERp57 expression levels. Furthermore, the expression levels of CRT, CNX and ERp57 were significantly upregulated when compared to that in the control group after SPS exposure by western blot analysis (P<0.05). RT‑qPCR analysis supported these results, indicating an upregulation of mRNA expression level. Taken together, the present findings suggest that SPS may induce changes to the expression of CRT, CNX and ERp57 in the amygdala of rats. The present study provides an insight into the effects of ER‑resident molecular chaperones in the amygdala participating in PTSD, and provides the experimental basis and a mechanism for the pathophysiology of PTSD.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

American Psychiatric Association: Diagnostic and Statistical Manual of Mental Disorders. 5th edition. Arlington, VA: American Psychiatric Publishing; pp. 271–280. 2013

2 

Mol SS, Arntz A, Metsemakers JF, Dinant GJ, Vilters-van Monfort PA and Knottnerus JA: Symptoms of post-traumatic stress disorder after non-traumatic events: Evidence from an open population study. Br J Psychiatry. 186:494–499. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Wen Y, Li B, Han F, Wang E and Shi Y: Dysfunction of calcium/calmodulin/CaM kinase IIα cascades in the medial prefrontal cortex in post-traumatic stress disorder. Mol Med Rep. 6:1140–1144. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Zhao D, Han F and Shi Y: Effect of glucose-regulated protein 94 and endoplasmic reticulum modulator caspase-12 in medial prefrontal cortex in a rat model of posttraumatic stress disorder. J Mol Neurosci. 54:147–155. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Xie J, Han F and Shi Y: The unfolded protein response is triggered in rat neurons of the dorsal raphe nucleus after single-pronged stress. Neurochem Res. 39:741–747. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Zhao W, Han F and Shi YX: IRE1α pathway of endoplasmic reticulum stress induces neuronal apoptosis in the locus coeruleus of rats under single prolonged stress. Prog Neuropsychopharmacol Biol Psychiatry. 69:11–18. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Groenendyk J and Michalak M: Endoplasmic reticulum quality control and apoptosis. Acta Biochim Pol. 52:381–395. 2005.PubMed/NCBI

8 

Breckenridge DG, Germain M, Mathai JP, Nguyen M and Shore GC: Regulation of apoptosis by endoplasmic reticulum pathways. Oncogene. 22:8608–8618. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Xiang J, Gu X, Qian S and Chen Z: Endoplasmic reticulum stress-mediated apoptosis involved in indirect recognition pathway blockade induces long-term heart allograft survival. J Biomed Biotechnol. 2010:7054312010. View Article : Google Scholar : PubMed/NCBI

10 

Lv S, Sun EC, Xu QY, Zhang JK and Wu DL: Endoplasmic reticulum stress-mediated autophagy contributes to bluetongue virus infection via the PERK-eIF2α pathway. Biochem Biophys Res Commun. 466:406–412. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Rao RV, Hermel E, Castro-Obregon S, del Rio G, Ellerby LM, Ellerby HM and Bredesen DE: Coupling endoplasmic reticulum stress to the cell death program. Mechanism of caspase activation. J Biol Chem. 276:33869–33874. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Boyce M and Yuan J: Cellular response to endoplasmic reticulum stress: A matter of life or death. Cell Death Differ. 13:363–373. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Prell T, Lautenschläger J and Grosskreutz J: Calcium-dependent protein folding in amyotrophic lateral sclerosis. Cell Calcium. 54:132–143. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Bernales S, Papa FR and Walter P: Intracellular signaling by the unfolded protein response. Annu Rev Cell Dev Biol. 22:487–508. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Jung J and Michalak M: Cell surface targeting of myelin oligodendrocyte glycoprotein (MOG) in the absence of endoplasmic reticulum molecular chaperones. Biochim Biophys Acta. 1813:1105–1110. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Williams DB: Beyond lectins: The calnexin/calreticulin chaperone system of the endoplasmic reticulum. J Cell Sci. 119:615–623. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Hebert DN and Molinari M: In and out of the ER: Protein folding, quality control, degradation, and related human diseases. Physiol Rev. 87:1377–1408. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Itakura M, Tsujimura J, Yamamori S, Ohkido T and Takahashi M: NMDA receptor-dependent recruitment of calnexin to the neuronal plasma membrane. Neurosci Lett. 550:173–178. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Ellgaard L, Riek R, Herrmann T, Güntert P, Braun D, Helenius A and Wüthrich K: NMR structure of the calreticulin P-domain. Proc Natl Acad Sci USA. 98:3133–3138. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Coe H and Michalak M: ERp57, a multifunctional endoplasmic reticulum resident oxidoreductase. Int J Biochem Cell Biol. 42:796–799. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Ellgaard L and Frickel EM: Calnexin, calreticulin, and ERp57: Teammates in glycoprotein folding. Cell Biochem Biophys. 39:223–247. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Szydlowska K and Tymianski M: Calcium, ischemia and excitotoxicity. Cell Calcium. 47:122–129. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Amunts K, Kedo O, Kindler M, Pieperhoff P, Mohlberg H, Shah NJ, Habel U, Schneider F and Zilles K: Cytoarchitectonic mapping of the human amygdala, hippocampal region and entorhinal cortex: Intersubject variability and probability maps. Anat Embryol (Berl). 210:343–352. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Davis M and Whalen PJ: The amygdala: Vigilance and emotion. Mol Psychiatry. 6:13–34. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Pape HC: Petrified or aroused with fear: The central amygdala takes the lead. Neuron. 67:527–529. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Solano-Castiella E, Anwander A, Lohmann G, Weiss M, Docherty C, Geyer S, Reimer E, Friederici AD and Turner R: Diffusion tensor imaging segments the human amygdala in vivo. Neuroimage. 49:2958–2965. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Young MB and Thomas SA: M1-muscarinic receptors promote fear memory consolidation via phospholipase C and the M-current. J Neurosci. 34:1570–1578. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Mihaljević S, Vuksan-Ćusa B, Marčinko D, Koić E, Kušević Z and Jakovljević M: Spiritual well-being, cortisol, and suicidality in croatian war veterans suffering from PTSD. J Relig Health. 50:464–473. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Yamamoto S, Morinobu S, Takei S, Fuchikami M, Matsuki A, Yamawaki S and Liberzon I: Single prolonged stress: Toward an animal model of posttraumatic stress disorder. Depress Anxiety. 26:1110–1117. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Wang W, Liu Y, Zheng H, Wang HN, Jin X, Chen YC, Zheng LN, Luo XX and Tan QR: A modified single-prolonged stress model for post-traumatic stress disorder. Neurosci Lett. 441:237–241. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Khan S and Liberzon I: Topiramate attenuated exaggerated acoustic startle in an animal model of PTSD. Psychopharmacology (Berl). 172:225–229. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Chavez CM, McGaugh JL and Weinberger NM: The basolateral amygdala modulates specific sensory memory representations in the cerebral cortex. Neurobiol Learn Mem. 91:382–392. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Xiao B, Han F and Shi YX: Dysfunction of Ca2+/CaM kinase IIalpha cascades in the amygdala in post-traumatic stress disorder. Int J Mol Med. 24:795–799. 2009.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xiao B, Wang JG, Han F and Shi YX: Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress. Mol Med Rep 17: 1099-1104, 2018.
APA
Xiao, B., Wang, J., Han, F., & Shi, Y. (2018). Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress. Molecular Medicine Reports, 17, 1099-1104. https://doi.org/10.3892/mmr.2017.7976
MLA
Xiao, B., Wang, J., Han, F., Shi, Y."Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress". Molecular Medicine Reports 17.1 (2018): 1099-1104.
Chicago
Xiao, B., Wang, J., Han, F., Shi, Y."Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress". Molecular Medicine Reports 17, no. 1 (2018): 1099-1104. https://doi.org/10.3892/mmr.2017.7976
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao B, Wang JG, Han F and Shi YX: Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress. Mol Med Rep 17: 1099-1104, 2018.
APA
Xiao, B., Wang, J., Han, F., & Shi, Y. (2018). Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress. Molecular Medicine Reports, 17, 1099-1104. https://doi.org/10.3892/mmr.2017.7976
MLA
Xiao, B., Wang, J., Han, F., Shi, Y."Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress". Molecular Medicine Reports 17.1 (2018): 1099-1104.
Chicago
Xiao, B., Wang, J., Han, F., Shi, Y."Effects of calcium-dependent molecular chaperones and endoplasmic reticulum in the amygdala in rats under single‑prolonged stress". Molecular Medicine Reports 17, no. 1 (2018): 1099-1104. https://doi.org/10.3892/mmr.2017.7976
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team