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Review

Advances in the T7 phage display system (Review)

  • Authors:
    • Xiangying Deng
    • Li Wang
    • Xiaolong You
    • Pei Dai
    • Yanhua Zeng
  • View Affiliations / Copyright

    Affiliations: Institute of Pathogenic Biology, Medical College, University of South China, Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan 421001, P.R. China
  • Pages: 714-720
    |
    Published online on: November 7, 2017
       https://doi.org/10.3892/mmr.2017.7994
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Abstract

The present review describes the advantages and updated applications of the T7 phage display system in bioscience and medical science. Current phage display systems are based on various bacteriophage vectors, including M13, T7, T4 and f1. Of these, the M13 phage display is the most frequently used, however, the present review highlights the advantages of the T7 system. As a phage display platform, M13 contains single‑stranded DNA, while the T7 phage consists of double‑stranded DNA, which exhibits increased stability and is less prone to mutation during replication. Additional characteristics of the T7 phage include the following: The T7 phage does not depend on a protein secretion pathway in the lytic cycle; expressed peptides and proteins are usually located on the C‑terminal region of capsid protein gp10B, which avoids problems associated with steric hindrance; and T7 phage particles exhibit high stability under various extreme conditions, including high temperature and low pH, which facilitates effective high‑throughput affinity elutriation. Recent applications of the T7 phage display system have been instrumental in uncovering mechanisms of molecular interaction, particularly in the fields of antigen discovery, vaccine development, protein interaction, and cancer diagnosis and treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Deng X, Wang L, You X, Dai P and Zeng Y: Advances in the T7 phage display system (Review). Mol Med Rep 17: 714-720, 2018.
APA
Deng, X., Wang, L., You, X., Dai, P., & Zeng, Y. (2018). Advances in the T7 phage display system (Review). Molecular Medicine Reports, 17, 714-720. https://doi.org/10.3892/mmr.2017.7994
MLA
Deng, X., Wang, L., You, X., Dai, P., Zeng, Y."Advances in the T7 phage display system (Review)". Molecular Medicine Reports 17.1 (2018): 714-720.
Chicago
Deng, X., Wang, L., You, X., Dai, P., Zeng, Y."Advances in the T7 phage display system (Review)". Molecular Medicine Reports 17, no. 1 (2018): 714-720. https://doi.org/10.3892/mmr.2017.7994
Copy and paste a formatted citation
x
Spandidos Publications style
Deng X, Wang L, You X, Dai P and Zeng Y: Advances in the T7 phage display system (Review). Mol Med Rep 17: 714-720, 2018.
APA
Deng, X., Wang, L., You, X., Dai, P., & Zeng, Y. (2018). Advances in the T7 phage display system (Review). Molecular Medicine Reports, 17, 714-720. https://doi.org/10.3892/mmr.2017.7994
MLA
Deng, X., Wang, L., You, X., Dai, P., Zeng, Y."Advances in the T7 phage display system (Review)". Molecular Medicine Reports 17.1 (2018): 714-720.
Chicago
Deng, X., Wang, L., You, X., Dai, P., Zeng, Y."Advances in the T7 phage display system (Review)". Molecular Medicine Reports 17, no. 1 (2018): 714-720. https://doi.org/10.3892/mmr.2017.7994
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