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Article Open Access

Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7

  • Authors:
    • Ling Wei
    • Bao‑Ming Li
    • Cheng‑Bin Wang
    • Zi‑Jia Kang
    • Jie Sun
    • Hui‑Juan Wu
    • Yong‑Zhi Lun
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, Beijing Centre for Physical and Chemical Analysis, Beijing 100094, P.R. China, Department of Clinical Laboratory Medicine, Chinese People's Liberation Army General Hospital & Medical School of Chinese People's Liberation Army, Beijing 100853, P.R. China, Department of Laboratory Medicine, School of Pharmacy and Medical Technology, Putian University, Putian, Fujian 351100, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 870-876
    |
    Published online on: November 7, 2017
       https://doi.org/10.3892/mmr.2017.7996
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Abstract

Escherichia coli (E. coli) O157:H7 is an important food‑borne pathogen with a low infective threshold and high resistance to treatment. There are currently a number of detection methods available, however, the majority are time‑consuming, complex and expensive, thus it is hard for these methods to be applied in routine detection. Therefore, there is urgent requirement to develop more sensitive, rapid and specific detective techniques. In the present study, an immuno‑biosensor based on the interference of load to the F0F1‑ATPase rotation, indicated by the fluorescence fluctuation, was constructed to detect O157:H7. The results demonstrated a good linear relationship (R2=0.9818) between antigen concentration (range, 102 cfu to 104 cfu) and the fluorescence intensity. The detection signals of the samples containing 102 cfu/well and 104 cfu/well E. coli O157:H7 were significantly stronger than the signal produced by the control sample (P<0.01). Due to its higher sensibility and simplicity when compared with the current methods applied, the results of the present study indicate a promising future for the application of this technique in detecting food source pathogens.
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View References

1 

Naugle AL, Holt KG, Levine P and Eckel R: Food safety and inspection service regulatory testing program for Escherichia coli O157:H7 in raw ground beef. J Food Prot. 68:462–468. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Wang HH and Wu RM: Survey on contamination status of food-borne pathogens in chilled broilers. Shiyong Yufang Yixue. 17:1314–1315. 2010.(In Chinese).

3 

Liu X, Zhang Y, Yue J, Jiang P and Zhang Z: F0F1-ATPase as biosensor to detect single virus. Biochem Biophys Res Commun. 342:1319–1322. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Wu HJWL and Liu QJ: Application of nano biosensortechnology in chloramphenicol detection. Food Sci. 31:167–170. 2010.(In Chinese).

5 

Wu HJ, Wei L, Lun YZ, Kang ZJ and Zhao L: The preliminary study of a rapid detecting technology for Listeria monocytogenes based on immunobiosensor. Zhongguo Weishengtai Xue Zazhi. 22:743–745. 2010.(In Chinese).

6 

Deng Z, Zhang Y, Yue J, Tang F and Wei Q: Green and orange CdTe quantum dots as effective pH-sensitive fluorescent probes for dual simultaneous and independent detection of viruses. J Phys Chem B. 111:12024–12031. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Yun Z, Zhengtao D, Jiachang Y, Fangqiong T and Qun W: Using cadmium telluride quantum dots as a proton flux sensor and applying to detect H9 avian influenza virus. Anal Biochem. 364:122–127. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Liu XL, Zhang XA, Cui YB, Yue JC, Luo ZY and Jiang PD: Mechanically driven proton conduction in single delta-free F0F1-ATPase. Biochem Biophys Res Commun. 347:752–757. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Zhao Y, Wang P, Wang F, Zhou H, Li W, Yue J and Ha Y: A novel biosensor regulated by the rotator of F0F1-ATPase to detect deoxynivalenolrapidly. Biochem Biophys Res Commun. 423:195–199. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Lu HT, Zhang Y, Yue JC, et al: Application of immuno-rotary biosensor based on FoF1-ATPase in Chromatophores for detecting clenbuterol. Food Science. 28:446–450. 2007.(In Chinese).

11 

Capaldi RA and Aggeler R: Mechanism of the F(1)F(0)-type ATP syn-thase, a biological rotary motor. Trends Biochem Sci. 27:154–160. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Karplus M and Gao YQ: Biomolecular motors: The F1-ATPase para-digm. Curr Opin Struct Biol. 14:250–259. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Clark LC Jr and Lyons C: Electrode systems for continuous monitoring in cardiovascular surgery. Ann N Y Acad Sci. 102:29–45. 1962. View Article : Google Scholar : PubMed/NCBI

14 

Wei L, Wu HJ, Li BM, et al: The pollution and detection research progress of four pathogenic bacterias. Food Sci. 32:302–306. 2011.(In Chinese).

15 

Wei L, Wu HJ, Lun YZ, Li BM, Gao LJ, Zhang XL and Kang ZJ: An immunobiosensor for rapid detection of Staphylococcus aureusenes. Zhongguo Shipin Weisheng Zazhi. 22:498–501. 2010.(In Chinese).

16 

Mousavi SL, Rasooli I, Nazarian S and Amani J: Simultaneous detection of Escherichia coli O157:H7, toxigenic Vibrio cholerae and Salmonella typhimurium by multiplex PCR. Iranian Journal of Clinical Infectious Diseases. 4:97–103. 2009.

17 

Zordan MD, Grafton MM, Acharya G, Reece LM, Cooper CL, Aronson AI, Park K and Leary JF: Detection of pathogenic E. coli O157:H7 by a hybrid microfluidic SPR and molecular imaging cytometry device. Cytometry A. 75:155–162. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Bahşi ZB, Buyukaksoy A, Aslan MH and Oral AY: DNA biosensors for E. coli O157:H7 detection in drinking water resources using sol-gel derived waveguides. South Biomed Eng Conf. 24:203–206. 2009.

19 

Oda M, Morita M, Unno H and Tanji Y: Rapid detection of Escherichia coli O157: H7 by using green fluorescent protein-labeled PP01 bacteriophage. Appl Environ Microbiol. 70:527–534. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Li F, Zhao C, Zhang W, Cui S, Meng J, Wu J and Zhang DY: Use of ramification amplification assay for detection of Escherichia coli O157:H7 and other E. J Clin Microbiol. 43:6086–6090. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Tang Z, Kotov NA and Giersig M: Spontaneous organization of single CdTe nanoparticles into luminescent nanowires. Science. 297:237–240. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Nanduri V, Bhunia AK, Tu SI, Paoli GC and Brewster JD: SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody. Biosens Bioelectron. 23:248–252. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Gao P, Xu G, Shi X, Yuan K and Tian J: Rapid detection of Staphylococcus aureus by a combination of monoclonal antibody-coated latex and capillary electrophoresis. Electrophoresis. 27:1784–1789. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Wu X, Liu H, Liu J, Haley KN, Treadway JA, Larson JP, Ge N, Peale F and Bruchez MP: Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat Biotechnol. 21:41–46. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Chan WC and Nie S: Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science. 281:2016–2018. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Nou X, Arthur TM, Bosilevac JM, Brichta-Harhay DM, Guerini MN, Kalchayanand N and Koohmaraie M: Improvement of immunomagnetic separation for Escherichia coli O157:H7 detection by the PickPen magnetic particle separation device. J Food Prot. 69:2870–2874. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Fu Z, Rogelj S and Kieft TL: Rapid detection of Escherichia coli O157:H7 by immunomagnetic separation and real-time PCR. Int J Food Microbiol. 99:47–57. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Chapman PA, Malo AT, Siddons CA and Harkin M: Use of commercial enzyme immunoassays and immunomagnetic separation systems for detecting Escherichia coli O157 in bovine fecal samples. Appl Environ Microbiol. 63:2549–2553. 1997.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wei L, Li BM, Wang CB, Kang ZJ, Sun J, Wu HJ and Lun YZ: Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7. Mol Med Rep 17: 870-876, 2018.
APA
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., & Lun, Y. (2018). Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7. Molecular Medicine Reports, 17, 870-876. https://doi.org/10.3892/mmr.2017.7996
MLA
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., Lun, Y."Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7". Molecular Medicine Reports 17.1 (2018): 870-876.
Chicago
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., Lun, Y."Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7". Molecular Medicine Reports 17, no. 1 (2018): 870-876. https://doi.org/10.3892/mmr.2017.7996
Copy and paste a formatted citation
x
Spandidos Publications style
Wei L, Li BM, Wang CB, Kang ZJ, Sun J, Wu HJ and Lun YZ: Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7. Mol Med Rep 17: 870-876, 2018.
APA
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., & Lun, Y. (2018). Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7. Molecular Medicine Reports, 17, 870-876. https://doi.org/10.3892/mmr.2017.7996
MLA
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., Lun, Y."Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7". Molecular Medicine Reports 17.1 (2018): 870-876.
Chicago
Wei, L., Li, B., Wang, C., Kang, Z., Sun, J., Wu, H., Lun, Y."Application of F0F1‑ATPase immuno‑biosensors for detecting Escherichia coli O157:H7". Molecular Medicine Reports 17, no. 1 (2018): 870-876. https://doi.org/10.3892/mmr.2017.7996
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