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Article Open Access

Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps

  • Authors:
    • Jong‑Yeup Kim
    • Dong‑Kyu Kim
    • Myeong Sang Yu
    • Min‑Ji Cha
    • Seong‑Lan Yu
    • Jaeku Kang
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea, Department of Otorhinolaryngology‑Head and Neck Surgery, Chuncheon Sacred Heart Hospital and Institute of New Frontier Research, Hallym University College of Medicine, Chuncheon, Gangwon 24253, Republic of Korea, Department of Otorhinolaryngology, Konkuk University School of Medicine, Chungju, North Chungcheong 27478, Republic of Korea, Department of Pharmacology, College of Medicine, Konyang University, Daejeon 35365, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1219-1227
    |
    Published online on: November 7, 2017
       https://doi.org/10.3892/mmr.2017.8001
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Abstract

Chronic rhinosinusitis (CRS) is a highly prevalent disease characterized by mucosal inflammation of the nose and paranasal sinuses. CRS can be divided into two main categories, CRS with nasal polyps (NPs; CRSwNP) and CRS without NPs (CRSsNP). Although the pathophysiology of CRS remains unclear, DNA methylation has been implicated in the etiology of CRSwNP. The aim of the present study was to elucidate whether DNA methylation of specific genes is involved in the development of NPs. In total, 18 individuals were included in the present study, and were divided into three groups: CRSwNP (n=7), CRSsNP (n=7) and healthy controls (n=4). NP tissues were obtained from the seven patients with CRSwNP and biopsies of the inferior turbinate mucosa from all three groups were used as controls. Methylated genes detected by methyl‑CpG‑binding domain sequencing were validated by methylation‑specific polymerase chain reaction (PCR), bisulfite sequencing, and reverse transcription‑quantitative PCR (RT‑qPCR). Methyl‑CpG‑binding domain sequencing identified 43,674 CpG islands in 518 genes. The promotor regions of 10 and 30 genes were hypermethylated and hypomethylated, respectively, in NP samples compared with controls. The top four genes with altered hypomethylation in NP tissues were, Keratin 19 (KRT19), nuclear receptor subfamily 2 group F member 2 (NR2F2), A Disintegrin‑like And Metallopeptidase (Reprolysin Type) with Thrombospondin type 1 motif 1 (ADAMTS1) and zinc finger protein 222 (ZNF222). RT‑qPCR demonstrated that the expression levels of KRT19, NR2F2 and ADAMTS1 were significantly increased in NP tissues; however, there was no difference in the levels of ZNF222 between NP and control tissues. Further studies are required to confirm the relevance of these epigenetic modifications in the mechanisms underlying NP formation.
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Copy and paste a formatted citation
Spandidos Publications style
Kim JY, Kim DK, Yu MS, Cha MJ, Yu SL and Kang J: Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps. Mol Med Rep 17: 1219-1227, 2018.
APA
Kim, J., Kim, D., Yu, M.S., Cha, M., Yu, S., & Kang, J. (2018). Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps. Molecular Medicine Reports, 17, 1219-1227. https://doi.org/10.3892/mmr.2017.8001
MLA
Kim, J., Kim, D., Yu, M. S., Cha, M., Yu, S., Kang, J."Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 17.1 (2018): 1219-1227.
Chicago
Kim, J., Kim, D., Yu, M. S., Cha, M., Yu, S., Kang, J."Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 17, no. 1 (2018): 1219-1227. https://doi.org/10.3892/mmr.2017.8001
Copy and paste a formatted citation
x
Spandidos Publications style
Kim JY, Kim DK, Yu MS, Cha MJ, Yu SL and Kang J: Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps. Mol Med Rep 17: 1219-1227, 2018.
APA
Kim, J., Kim, D., Yu, M.S., Cha, M., Yu, S., & Kang, J. (2018). Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps. Molecular Medicine Reports, 17, 1219-1227. https://doi.org/10.3892/mmr.2017.8001
MLA
Kim, J., Kim, D., Yu, M. S., Cha, M., Yu, S., Kang, J."Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 17.1 (2018): 1219-1227.
Chicago
Kim, J., Kim, D., Yu, M. S., Cha, M., Yu, S., Kang, J."Role of epigenetics in the pathogenesis of chronic rhinosinusitis with nasal polyps". Molecular Medicine Reports 17, no. 1 (2018): 1219-1227. https://doi.org/10.3892/mmr.2017.8001
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