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Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii

  • Authors:
    • Zhenying Zhang
    • Binbin Hou
    • Yong Zhuo Wu
    • Ying Wang
    • Xiaoming Liu
    • Shixin Han
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, University of Hong Kong‑Shenzhen Hospital, Shenzhen, Guangdong 518053, P.R. China, Department of Dermatology, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116044, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 721-728
    |
    Published online on: November 7, 2017
       https://doi.org/10.3892/mmr.2017.8005
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Abstract

Sporothrix schenckii is a pathogenic dimorphic fungus with a global distribution. It grows in a multicellular hyphal form at 25˚C and a unicellular yeast form at 37˚C. The morphological switch from mold to yeast form is obligatory for establishing pathogenicity in S. schenckii. Two‑component signaling systems are utilized by eukaryotes to sense and respond to external environmental changes. DRK1is a hybrid histidine kinase, which functions as a global regulator of dimorphism and virulence in Blastomyces dermatitidis and Histoplasma capsulatum. An intracellular soluble hybrid histidine kinase, homologous to DRK1 in B. dermatitidis, has previously been identified in S. schenckii and designated as SsDRK1. In the present study, the function of SsDRK1 was investigated using double stranded RNA interference mediated by Agrobacterium tumefaciens. SsDRK1 was demonstrated to be required for normal asexual development, yeast‑phase cell formation, cell wall composition and integrity, melanin synthesis, transcription of the morphogenesis‑associated gene Ste20 that is involved in the high osmolarity glycerol/mitogen‑activated protein kinase pathway, and pathogenicity of S. schenckii in a murine model of cutaneous infection. Further investigations into the signals SsDRK1 responds to, and the interactions of upstream transmembrane hybrid histidine kinases with SsDRK1, are required to uncover novel targets for anti‑fungal therapies.
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View References

1 

Morris-Jones R: Sporotrichosis. Clin Exp Dermatol. 27:427–431. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Cooney NM and Klein BS: Fungal adaptation to the mammalian host: It is a new world, after all. Curr Opin Microbiol. 11:511–516. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Bahn YS, Xue C, Idnurm A, Rutherford JC, Heitman J and Cardenas ME: Sensing the environment: Lessons from fungi. Nat Rev Microbiol. 5:57–69. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Bahn YS: Master and commander in fungal pathogens: The two-component system and the HOG signaling pathway. Eukaryot Cell. 7:2017–2036. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Brewster JL, de Valoir T, Dwyer ND, Winter E and Gustin MC: An osmosensing signal transduction pathway in yeast. Science. 259:1760–1763. 1993. View Article : Google Scholar : PubMed/NCBI

6 

Brown SM, Campbell LT and Lodge JK: Cryptococcus neoformans, a fungus under stress. Curr Opin Microbiol. 10:320–325. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Bahn YS, Kojima K, Cox GM and Heitman J: A unique fungal two-component system regulates stress responses, drug sensitivity, sexual development, and virulence of Cryptococcus neoformans. Mol Biol Cell. 17:3122–3135. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Nemecek JC, Wüthrich M and Klein BS: Global control of dimorphism and virulence in fungi. Science. 312:583–588. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Cheung J and Hendrickson WA: Sensor domains of two-component regulatory systems. Curr Opin Microbiol. 13:116–123. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Hou B, Zhang Z, Zheng F and Liu X: Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii. Int J Mol Med. 31:99–104. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Sambrook J, Fritsch EF and Maniatis T: Molecular cloning: A laboratory manual. 3rd edition. Cold spring harbor laboratory; New York, NY, USA: 2001

12 

Zhang P, Xu B, Wang Y, Li Y, Qian Z, Tang S, Huan S and Ren S: Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the fungus Penicillium marneffei. Mycol Res. 112:943–949. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Garrison RG, Boyd KS and Mariat F: Ultrastructural studies of the mycelium-to yeast transformation of sporothrix schenckii. J Bacteriol. 124:959–968. 1975.PubMed/NCBI

14 

Wang F, Tao J, Qian Z, You S, Dong H, et al: A histidine kinase PmHHK1 regulates polar growth, sporulation and cell wall composition in the dimorphic fungus Penicillium marneffei. Mycological Research. 113:915–923. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Davis RH and De Serres FJ: Genetic and microbiological research techniques for neurospora crassa. Methods Enzymol. 17:79–143. 1970. View Article : Google Scholar

16 

De Nobel JG, Klis FM, Priem J, Munnik T and van den Ende H: The glucanase-soluble mannoproteins limit cell wall porosity in Saccharomyces cerevisiae. Yeast. 6:491–499. 1990. View Article : Google Scholar : PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Kong X, Xiao T, Lin J, Wang Y and Chen HD: Relationships among genotypes, virulence and clinical forms of sporothrix schenckii infection. Clin Microbiol Infect. 12:1077–1081. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Viaud M, Fillinger S, Liu W, Polepalli JS, Le Pêcheur P, Kunduru AR, Leroux P and Legendre L: A class III histidine kinase acts as a novel virulence factor in botrytis cinerea. Mol Plant Microbe Interact. 19:1042–1050. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Vargas-Pérez I, Sánchez O, Kawasaki L, Georgellis D and Aguirre J: Response regulators SrrA and SskA are central components of a phosphorelay system involved in stress signal transduction and asexual sporulation in Aspergillus nidulans. Eukaryot Cell. 6:1570–1583. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Boyce KJ, Schreider L, Kirszenblat L and Andrianopoulos A: The two-component histidine kinases DrkA and SlnA are required for in vivo growth in the human pathogen Penicillium marneffei. Mol Microbiol. 82:1164–1184. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Svoboda A and Trujillo-Gonzalez A: Sporothrix schenckii-a freeze-fracture study. J Basic Microbiol. 30:371–378. 1990. View Article : Google Scholar : PubMed/NCBI

23 

Kruppa M, Jabra-Rizk MA, Meiller TF and Calderone R: The histidine kinases of Candida albicans: Regulation of cell wall mannan biosynthesis. FEMS Yeast Res. 4:409–416. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Drutz DJ and Frey CL: Intracellular and extracellular defenses of human phagocytes against Blastomyces dermatitidis conidia and yeasts. J Lab Clin Med. 105:737–750. 1985.PubMed/NCBI

25 

Klein BS and Tebbets B: Dimorphism and virulence in fungi. Curr Opin Microbiol. 10:314–319. 2007. View Article : Google Scholar : PubMed/NCBI

26 

Revankar SG and Sutton DA: Melanized fungi in human disease. Clin Microbiol Rev. 23:884–928. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Madrid H, Cano J, Gené J, Bonifaz A, Toriello C and Guarro J: Sporothrix globosa, a pathogenic fungus with widespread geographical distribution. Rev Iberoam Micol. 26:218–222. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Romero-Martinez R, Wheeler M, Guerrero-Plata A, Rico G and Torres-Guerrero H: Biosynthesis and functions of melanin in Sporothrix schenckii. Infect Immun. 68:3696–3703. 2000. View Article : Google Scholar : PubMed/NCBI

29 

Morris-Jones R, Youngchim S, Gomez BL, Aisen P, Hay RJ, Nosanchuk JD, Casadevall A and Hamilton AJ: Synthesis of melanin-like pigments by Sporothrix schenckii in vitro and during mammalian infection. Infect Immun. 71:4026–4033. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Bernhardt J, Herman D, Sheridan M and Calderone R: Adherence and invasion studies of Candidia albicans strains, utilizing in vitro models of esophageal candidiasis. J Infect Dis. 184:1170–1175. 2001. View Article : Google Scholar : PubMed/NCBI

31 

Rappleye CA, Engle JT and Goldman WE: RNA interference in Histoplasma capsulatum demonstrates a role for alpha-(1,3)-glucan in virulence. Mol Microbiol. 53:153–165. 2004. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Hou B, Wu YZ, Wang Y, Liu X and Han S: Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii. Mol Med Rep 17: 721-728, 2018.
APA
Zhang, Z., Hou, B., Wu, Y.Z., Wang, Y., Liu, X., & Han, S. (2018). Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii. Molecular Medicine Reports, 17, 721-728. https://doi.org/10.3892/mmr.2017.8005
MLA
Zhang, Z., Hou, B., Wu, Y. Z., Wang, Y., Liu, X., Han, S."Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii". Molecular Medicine Reports 17.1 (2018): 721-728.
Chicago
Zhang, Z., Hou, B., Wu, Y. Z., Wang, Y., Liu, X., Han, S."Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii". Molecular Medicine Reports 17, no. 1 (2018): 721-728. https://doi.org/10.3892/mmr.2017.8005
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Hou B, Wu YZ, Wang Y, Liu X and Han S: Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii. Mol Med Rep 17: 721-728, 2018.
APA
Zhang, Z., Hou, B., Wu, Y.Z., Wang, Y., Liu, X., & Han, S. (2018). Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii. Molecular Medicine Reports, 17, 721-728. https://doi.org/10.3892/mmr.2017.8005
MLA
Zhang, Z., Hou, B., Wu, Y. Z., Wang, Y., Liu, X., Han, S."Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii". Molecular Medicine Reports 17.1 (2018): 721-728.
Chicago
Zhang, Z., Hou, B., Wu, Y. Z., Wang, Y., Liu, X., Han, S."Two‑component histidine kinase DRK1 is required for pathogenesis in Sporothrix schenckii". Molecular Medicine Reports 17, no. 1 (2018): 721-728. https://doi.org/10.3892/mmr.2017.8005
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