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Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement

  • Authors:
    • Liping Jiang
    • Zhen Tang
  • View Affiliations / Copyright

    Affiliations: Department of Orthodontics, Affiliated Stomatological Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1499-1506
    |
    Published online on: November 10, 2017
       https://doi.org/10.3892/mmr.2017.8021
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Abstract

The present study aimed to investigate the expression and regulation of extracellular signal‑regulated kinase (ERK)1/2 and p38 mitogen‑activated protein kinase (MAPK) signaling pathways in periodontal tissue remodeling of orthodontic tooth movement. Sprague Dawley rats with orthodontic tooth movement were generated. After tension stress for 1, 3, 5, 7 and 14 days, the protein and mRNA expression levels of ERK1/2 and p38 in periodontal tissue were determined by western blotting and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), respectively. Primary human periodontal ligament cells (hPDLCs) were separated and characterized. Following exposure to centrifugal force for 1, 2, 6, 8 and 12 h, the protein expression levels of ERK1/2 and p38 MAPK, and the mRNA expression levels of ERK1/2, p38 and osteogenesis associated‑genes [including alkaline phosphatase (ALP), osteopontin (OPN), collagen I (Col I), osteocalcin (OCN) and bone sialoprotein (BSP)] were measured. The protein expression levels of ERK1/2 and p38 MAPK in periodontal tissue and hPDLCs treated with stress were similar to those in the control groups. However, compared with the control, the phosphorylation and mRNA expression levels of the genes encoding ERK1/2 and p38 MAPK in orthodontic periodontal tissue and forced hPDLCs were elevated. These increases reached a peak at 5 days for orthodontic periodontal tissue and at 6 h for forced hPDLCs. In forced hPDLCs, the mRNA expression levels of ALP, OPN, Col I, OCN and BSP were notably and continuously upregulated in a time‑dependent manner. In addition, hPDLCs were treated with the ERK1/2 inhibitor, PD098059, and the p38 MAPK inhibitor, SB203580, and the mRNA expression levels of the osteogenesis associated‑genes were then measured using RT‑qPCR. Following treatment with the ERK1/2 inhibitor and p38 MAPK inhibitor, the mRNA expression levels of ALP, OPN, Col I, OCN and BSP were significantly downregulated. In conclusion, ERK1/2 and p38 MAPK signaling pathways may be positively and closely associated with periodontal tissue remodeling of orthodontic tooth movement.
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View References

1 

Liu X and Li Z: Research progress of biochemical markers changes of bone turnover in orthodontic tooth movement. J Oral Sci Res. 29:487–489. 2013.

2 

Adusumilli S, Yalamanchi L and Yalamanchili PS: Periodontally accelerated osteogenic orthodontics: An interdisciplinary approach for faster orthodontic therapy. J Pharm Bioallied Sci. 6 Suppl 1:S2–S5. 2014. View Article : Google Scholar :

3 

Zhou H and Cai P: Research progress on periodontal conditions in subjects following orthodontic therapy. Int J Oral Sci. 38:109–111. 2011.

4 

Peng P and Wang W: Biological behavior of periodontal ligament in orthodontic tooth movement. Beijing J Stom. 20:354–357. 2012.

5 

Bao X and Hu M: Research progress on mechanisms and regulations of bone resorption in orthodontic tooth movement. Int J Oral Sci. 39:187–189. 2012.

6 

Kim IS, Jeong BC, Kim OS, Kim YJ, Lee SE, Lee KN, Koh JT and Chung HJ: Lactone form 3-hydroxy-3-methylglutaryl-coenzyme a reductase inhibitors (statins) stimulate the osteoblastic differentiation of mouse periodontal ligament cells via the ERK pathway. J Periodontal Res. 46:204–213. 2011. View Article : Google Scholar

7 

Chen X, Shi J, Ye Q, Cai X and Zhang T: Effect of p38 MAPK signaling pathway on BMP-2-induced osteogenic differentiation of human dental follicle cells. Chin J Cell Bio. 35:816–823. 2013.(In Chinese). View Article : Google Scholar

8 

Liu C and Sun J: Hydrolyzed tilapia fish collagen induces osteogenic differentiation of human periodontal ligament cells. Biomed Mater. 10:0650202015. View Article : Google Scholar

9 

Tsutsumi T, Kajiya H, Fukawa T, Sasaki M, Nemoto T, Tsuzuki T, Takahashi Y, Fujii S, Maeda H and Okabe K: The potential role of transient receptor potential type A1 as a mechanoreceptor in human periodontal ligament cells. Eur J Oral Sci. 121:538–544. 2013. View Article : Google Scholar

10 

Li L, Han M, Li S, Wang L and Xu Y: Cyclic tensile stress during physiological occlusal force enhances osteogenic differentiation of human periodontal ligament cells via ERK1/2-Elk1 MAPK pathway. DNA Cell Biol. 32:488–497. 2013. View Article : Google Scholar :

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T) method. Methods. 25:402–408. 2001. View Article : Google Scholar

12 

Gerdts J, Summers DW, Milbrandt J and DiAntonio A: Axon self-destruction: New links among SARM1, MAPKs, and NAD+ metabolism. Neuron. 89:449–460. 2016. View Article : Google Scholar :

13 

Kim HK, Kim MG and Leem KH: Effects of egg yolk-derived peptide on osteogenic gene expression and MAPK activation. Molecules. 19:12909–12924. 2014. View Article : Google Scholar

14 

Li C, Yang X, He Y, Ye G, Li X, Zhang X, Zhou L and Deng F: Bone morphogenetic protein-9 induces osteogenic differentiation of rat dental follicle stem cells in P38 and ERK1/2 MAPK dependent manner. Int J Med Sci. 9:862–871. 2012. View Article : Google Scholar :

15 

Xiao G, Jiang D, Gopalakrishnan R and Franceschi RT: Fibroblast growth factor 2 induction of the osteocalcin gene requires MAPK activity and phosphorylation of the osteoblast transcription factor, Cbfa1/Runx2. J Biol Chem. 277:36181–36187. 2002. View Article : Google Scholar

16 

Kang KL, Lee SW, Ahn YS, Kim SH and Kang YG: Bioinformatic analysis of responsive genes in two-dimension and three-dimension cultured human periodontal ligament cells subjected to compressive stress. J Periodontal Res. 48:87–97. 2013. View Article : Google Scholar

17 

Pavlidis D, Bourauel C, Rahimi A, Götz W and Jäger A: Proliferation and differentiation of periodontal ligament cells following short-term tooth movement in the rat using different regimens of loading. Eur J Orthod. 31:565–571. 2009. View Article : Google Scholar

18 

An J, Yang H, Zhang Q, Liu C, Zhao J, Zhang L and Chen B: Natural products for treatment of osteoporosis: The effects and mechanisms on promoting osteoblast-mediated bone formation. Life Sci. 147:46–58. 2016. View Article : Google Scholar

19 

Guo Z, Kang S, Chen D, Wu Q, Wang S, Xie W, Zhu X, Baxter SW, Zhou X, Jurat-Fuentes JL and Zhang Y: MAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback moth. PLoS Genet. 11:e10051242015. View Article : Google Scholar :

20 

Zhang R, Zhang Z, Pan X, Huang X, Huang Z and Zhang G: ATX–LPA axis induces expression of OPN in hepatic cancer cell SMMC7721. Anat Rec (Hoboken). 294:406–411. 2011. View Article : Google Scholar

21 

Wang C: The effect of Asiaticoside on the expression of TGF-β on thIand Col III in rats after myocardial infarction. J Comm Medi. 2015.(In Chinese).

22 

Rocha ÉD, de Brito NJ, Dantas MM, Silva Ade A, Almeida Md and Brandão-Neto J: Effect of zinc supplementation on GH, IGF1, IGFBP3, OCN, and ALP in non-zinc-deficient children. J Am Coll Nutr. 34:290–299. 2015. View Article : Google Scholar

23 

Bouet G, Bouleftour W, Juignet L, Linossier MT, Thomas M, Vanden-Bossche A, Aubin JE, Vico L, Marchat D and Malaval L: The impairment of osteogenesis in bone sialoprotein (BSP) knockout calvaria cell cultures is cell density dependent. PLoS One. 10:e01174022015. View Article : Google Scholar :

24 

Xu L and Kong Q: Research progress of key signaling pathways in osteoblast differentiation and bone formation regulation. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 28:1484–1489. 2014.(In Chinese).

25 

Yang Z, Ren L, Deng F, Wang Z and Song J: Low-intensity pulsed ultrasound induces osteogenic differentiation of human periodontal ligament cells through activation of bone morphogenetic protein-smad signaling. J Ultrasound Med. 33:865–873. 2014. View Article : Google Scholar

26 

Matsuzawa M, Sheu TJ, Lee YJ, Chen M, Li TF, Huang CT, Holz JD and Puzas JE: Putative signaling action of amelogenin utilizes the Wnt/beta-catenin pathway. J Periodontal Res. 44:289–296. 2009. View Article : Google Scholar

27 

Tang M, Peng Z, Mai Z, Chen L, Mao Q, Chen Z, Chen Q, Liu L, Wang Y and Ai H: Fluid shear stress stimulates osteogenic differentiation of human periodontal ligament cells via the extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinase signaling pathways. J Periodontol. 85:1806–1813. 2014. View Article : Google Scholar

28 

Lee SK, Chung JH, Choi SC, Auh QS, Lee YM, Lee SI and Kim EC: Sodium hydrogen sulfide inhibits nicotine and lipopolysaccharide-induced osteoclastic differentiation and reversed osteoblastic differentiation in human periodontal ligament cells. J Cell Biochem. 114:1183–1193. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Jiang L and Tang Z: Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement. Mol Med Rep 17: 1499-1506, 2018.
APA
Jiang, L., & Tang, Z. (2018). Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement. Molecular Medicine Reports, 17, 1499-1506. https://doi.org/10.3892/mmr.2017.8021
MLA
Jiang, L., Tang, Z."Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement". Molecular Medicine Reports 17.1 (2018): 1499-1506.
Chicago
Jiang, L., Tang, Z."Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement". Molecular Medicine Reports 17, no. 1 (2018): 1499-1506. https://doi.org/10.3892/mmr.2017.8021
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang L and Tang Z: Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement. Mol Med Rep 17: 1499-1506, 2018.
APA
Jiang, L., & Tang, Z. (2018). Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement. Molecular Medicine Reports, 17, 1499-1506. https://doi.org/10.3892/mmr.2017.8021
MLA
Jiang, L., Tang, Z."Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement". Molecular Medicine Reports 17.1 (2018): 1499-1506.
Chicago
Jiang, L., Tang, Z."Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement". Molecular Medicine Reports 17, no. 1 (2018): 1499-1506. https://doi.org/10.3892/mmr.2017.8021
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