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Article

Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses

  • Authors:
    • Yan Xiao
    • Jie Wu
    • Yongjun Yuan
    • Xiaohua Guo
    • Bo Chen
    • Qiaobing Huang
  • View Affiliations / Copyright

    Affiliations: Department of Pathophysiology, Key Laboratory for Shock and Microcirculation Research of Guangdong, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
  • Pages: 1933-1939
    |
    Published online on: November 13, 2017
       https://doi.org/10.3892/mmr.2017.8029
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Abstract

It was previously reported that low‑dose sphingosine‑1‑phosphate (S1P) enhanced endothelial barrier integrity, whereas high‑dose S1P induced endothelial monolayer hyperpermeability responses. A number of studies have revealed the underlying molecular mechanisms of the physiological‑dose of S1P on barrier‑protective effect. However, little work has been performed to determine the effect of S1P‑induced endothelial barrier responses. In the present study, the role of moesin phosphorylation in excessive S1P‑induced endothelial hyperpermeability was investigated by western blotting, fluorescence staining and transendothelial electrical resistance detection. It was revealed that S1P induced moesin phosphorylation in a time‑ and concentration‑dependent manner. In addition, it was confirmed that high‑dose S1P‑induced moesin phosphorylation occurred via S1P receptor 2 (S1PR2). Moesin phosphorylation was required for S1P‑induced F‑actin rearrangement and endothelial barrier disruption. The results suggested that the S1PR2‑moesin axis is involved in high‑dose S1P‑induced endothelial barrier responses. The results of the present study may provide novel therapeutic targets for endothelial injury‑associated vascular disorders.
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Copy and paste a formatted citation
Spandidos Publications style
Xiao Y, Wu J, Yuan Y, Guo X, Chen B and Huang Q: Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses. Mol Med Rep 17: 1933-1939, 2018.
APA
Xiao, Y., Wu, J., Yuan, Y., Guo, X., Chen, B., & Huang, Q. (2018). Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses. Molecular Medicine Reports, 17, 1933-1939. https://doi.org/10.3892/mmr.2017.8029
MLA
Xiao, Y., Wu, J., Yuan, Y., Guo, X., Chen, B., Huang, Q."Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses". Molecular Medicine Reports 17.1 (2018): 1933-1939.
Chicago
Xiao, Y., Wu, J., Yuan, Y., Guo, X., Chen, B., Huang, Q."Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses". Molecular Medicine Reports 17, no. 1 (2018): 1933-1939. https://doi.org/10.3892/mmr.2017.8029
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao Y, Wu J, Yuan Y, Guo X, Chen B and Huang Q: Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses. Mol Med Rep 17: 1933-1939, 2018.
APA
Xiao, Y., Wu, J., Yuan, Y., Guo, X., Chen, B., & Huang, Q. (2018). Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses. Molecular Medicine Reports, 17, 1933-1939. https://doi.org/10.3892/mmr.2017.8029
MLA
Xiao, Y., Wu, J., Yuan, Y., Guo, X., Chen, B., Huang, Q."Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses". Molecular Medicine Reports 17.1 (2018): 1933-1939.
Chicago
Xiao, Y., Wu, J., Yuan, Y., Guo, X., Chen, B., Huang, Q."Effect of moesin phosphorylation on high‑dose sphingosine‑1‑phosphate‑induced endothelial responses". Molecular Medicine Reports 17, no. 1 (2018): 1933-1939. https://doi.org/10.3892/mmr.2017.8029
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