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17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway

  • Authors:
    • Lianyong Liu
    • Lin Zhou
    • Xiaorong Yang
    • Qi Liu
    • Ling Yang
    • Chao Zheng
    • Yongling Zhao
    • Zhenlin Zhang
    • Xiaohong Luo
  • View Affiliations / Copyright

    Affiliations: Medical College of Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Endocrinology, Punan Hospital of Pudong New District, Shanghai 200125, P.R. China, Metabolic Bone Disease and Genetic Research Unit, Department of Osteoporosis and Bone Diseases, Shanghai Key Clinical Center for Metabolic Disease, Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1609-1616
    |
    Published online on: November 14, 2017
       https://doi.org/10.3892/mmr.2017.8042
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Abstract

The present study aimed to investigate whether 17β‑estradiol (E2) exerts protective effects on bone deterioration induced by ovariectomy (OVX) through the ephA2/ephrinA2 signaling pathway in rats. Female rats were subjected to OVX, sham surgeryor OVX+E2 treatment. Levels of biomarkers were measured in serum and urine. Hematoxylin and eosin staining was performed on paraffin‑embedded bone sections. Expression of genes and proteins was analyzed by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. Bone mineral density (BMD) was analyzed by dual‑energy X‑ray absorptiometry. Trabecular bone microarchitecture was also evaluated. Osteoclastogenesis was induced by in vitro culturing with mouse receptor activator of nuclear factor κB ligand (RANKL) and macrophage colony‑stimulating factor 1. small interfering RNA was designed to knockdown ehpA2 receptor and its ligand ephrinA2. Results of the present study demonstrated that E2 had suppressive effects on OVX‑induced body weight gain and bone turnover factors in serum and urine. E2 inhibited the bone resorption function of osteoclasts by inhibiting the production of tartrate‑resistant acid phosphatase‑5b and RANKL, and induced bone formation function of osteoblasts by prompting runt‑related transcription factor 2, Sp7 transcription factor and collagen alpha‑1(I) chain expression in bone marrow cells. E2 treatment significantly increased the tibia BMD and prevented the deterioration of trabecular microarchitecture compared with the OVX group. Moreover, E2 significantly decreased the OVX‑stimulated expression of ephA2 and ephrinA2. EphA2 or ephrin A2 knockdown significantly suppressed osteoclastogenesis in vitro. In conclusion, E2 can attenuate OVX‑induced bone deterioration partially through the suppression of the ephA2/ephrinA2 signaling pathway. Therefore EphA2/ephrinA2 signaling pathway may be a potential target for osteoporosis treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Zhou L, Yang X, Liu Q, Yang L, Zheng C, Zhao Y, Zhang Z and Luo X: 17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway. Mol Med Rep 17: 1609-1616, 2018.
APA
Liu, L., Zhou, L., Yang, X., Liu, Q., Yang, L., Zheng, C. ... Luo, X. (2018). 17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway. Molecular Medicine Reports, 17, 1609-1616. https://doi.org/10.3892/mmr.2017.8042
MLA
Liu, L., Zhou, L., Yang, X., Liu, Q., Yang, L., Zheng, C., Zhao, Y., Zhang, Z., Luo, X."17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway". Molecular Medicine Reports 17.1 (2018): 1609-1616.
Chicago
Liu, L., Zhou, L., Yang, X., Liu, Q., Yang, L., Zheng, C., Zhao, Y., Zhang, Z., Luo, X."17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway". Molecular Medicine Reports 17, no. 1 (2018): 1609-1616. https://doi.org/10.3892/mmr.2017.8042
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Zhou L, Yang X, Liu Q, Yang L, Zheng C, Zhao Y, Zhang Z and Luo X: 17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway. Mol Med Rep 17: 1609-1616, 2018.
APA
Liu, L., Zhou, L., Yang, X., Liu, Q., Yang, L., Zheng, C. ... Luo, X. (2018). 17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway. Molecular Medicine Reports, 17, 1609-1616. https://doi.org/10.3892/mmr.2017.8042
MLA
Liu, L., Zhou, L., Yang, X., Liu, Q., Yang, L., Zheng, C., Zhao, Y., Zhang, Z., Luo, X."17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway". Molecular Medicine Reports 17.1 (2018): 1609-1616.
Chicago
Liu, L., Zhou, L., Yang, X., Liu, Q., Yang, L., Zheng, C., Zhao, Y., Zhang, Z., Luo, X."17β-estradiol attenuates ovariectomy‑induced bone deterioration through the suppression of the ephA2/ephrinA2 signaling pathway". Molecular Medicine Reports 17, no. 1 (2018): 1609-1616. https://doi.org/10.3892/mmr.2017.8042
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