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Article

Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study

  • Authors:
    • Chiun‑Lang Wang
    • Po‑Sheng Yang
    • Jeng‑Ting Tsao
    • Thanasekaran Jayakumar
    • Meng‑Jiy Wang
    • Joen‑Rong Sheu
    • Duen‑Suey Chou
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Min‑Sheng General Hospital, Taoyuan 33044, Taiwan, R.O.C., Department of Surgery, MacKay Memorial Hospital and Mackay Medical College, Taipei 10449, Taiwan, R.O.C., Division of Allergy and Immunology, Department of Internal Medicine, Cathay General Hospital, Taipei 10630, Taiwan, R.O.C., Department of Pharmacology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan, R.O.C., Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 10607, Taiwan, R.O.C.
  • Pages: 2061-2069
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    Published online on: November 14, 2017
       https://doi.org/10.3892/mmr.2017.8058
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Abstract

Oxygen free radicals have been implicated in the pathogenesis of toxic liver injury and are thought to be involved in cardiac dysfunction in the cirrhotic heart. Therefore, direct evidence for the electron spin resonance (ESR) detection of how D‑galactosamine (GalN), an established experimental hepatotoxic substance, induced free radicals formation in platelets and primary hepatocytes is presented in the present study. ESR results demonstrated that GalN induced hydroxyl radicals (OH•) in a resting human platelet suspension; however, radicals were not produced in a cell free Fenton reaction system. The GalN‑induced OH• formation was significantly inhibited by the cyclooxygenase (COX) inhibitor indomethasin, though it was not affected by the lipoxygenase (LOX) or cytochrome P450 inhibitors, AA861 and 1‑aminobenzotriazole (ABT), in platelets. In addition, the present study demonstrated that baicalein induced semiquinone free radicals in platelets, which were significantly reduced by the COX inhibitor without affecting the formed OH•. In the mouse primary hepatocytes, the formation of arachidonic acid (AA) induced carbon‑centered radicals that were concentration dependently enhanced by GalN. These radicals were inhibited by AA861, though not affected by indomethasin or ABT. In addition, GalN did not induce platelet aggregation prior to or following collagen pretreatment in human platelets. The results of the present study indicated that GalN and baicalein may induce OH• by COX and LOX in human platelets. GalN also potentiated AA induced carbon‑centered radicals in hepatocytes via cytochrome P450. The present study presented the role of free radicals in the pathophysiological association between platelets and hepatocytes.
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View References

1 

Boelsterli UA and Lim PL: Mitochondrial abnormalities-a link to idiosyncratic drug hepatotoxicity? Toxicol Appl Pharmacol. 220:92–107. 2007. View Article : Google Scholar

2 

Gómez-Lechón MJ, Tolosa L, Castell JV and Donato MT: Mechanism-based selection of compounds for the development of innovative in vitro approaches tohepatotoxicity studies in the LIINTOP project. Toxicol In Vitro. 24:1879–1889. 2010. View Article : Google Scholar

3 

Keppler D, Lesch R, Reutter W and Decker K: Experimental hepatitis induced by D-galactosamine. Exp Mol Pathol. 9:279–290. 1968. View Article : Google Scholar

4 

Quintero A, Pedraza CA, Siendones E, ElSaid Kamal AM, Colell A, García-Ruiz C, Montero JL, De la Mata M, Fernández-Checa JC, Miño G and Muntané J: PGE1 protection against apoptosis induced by D-galactosamine is not related to the modulation of intracellular free radical production in primary culture of rat hepatocytes. Free Radic Res. 36:345–355. 2002. View Article : Google Scholar

5 

Lekić N, Cerný D, Hořínek A, Provazník Z, Martínek J and Farghali H: Differential oxidative stress responses to D-galactosamine-lipopolysaccharide hepatotoxicity based on real time PCR analysis of selected oxidant/antioxidant andapoptotic gene expressions in rat. Physiol Res. 60:549–558. 2011.

6 

Choi JH, Kang JW, Kim DW, Sung YK and Lee SM: Protective effects of Mg-CUD against D-galactosamine-induced hepatotoxicity in rats. Eur J Pharmacol. 657:138–143. 2011. View Article : Google Scholar

7 

Bruck R, Aeed H, Avni Y, Shirin H, Matas Z, Shahmurov M, Avinoach I, Zozulya G, Weizman N and Hochman A: Melatonin inhibits nuclear factor kappa B activation and oxidative stress and protects against thioacetamide induced liver damage in rats. J Hepatol. 40:86–93. 2004. View Article : Google Scholar

8 

von Sonntag C: Free-radical-induced DNA damage and its repair. Springer-Verlag; Berlin: 2006, View Article : Google Scholar

9 

Black M: Acetaminophen hepatotoxicity. Annu Rev Med. 35:577–593. 1984. View Article : Google Scholar

10 

Lam FW, Vijayan KV and Rumbaut RE: Platelets and their interactions with other immune cells. Compr Physiol. 5:1265–1280. 2015. View Article : Google Scholar :

11 

van Golen RF, van Gulik TM and Heger M: Mechanistic overview of reactive species-induced degradation of the endothelial glycocalyx during hepatic ischemia/reperfusion injury. Free Radic Biol Med. 52:1382–1402. 2012. View Article : Google Scholar

12 

Khandoga A, Biberthaler P, Enders G, Axmann S, Hutter J, Messmer K and Krombach F: Platelet adhesion mediated by fibrinogen-intercelllular adhesion molecule-1 binding induces tissue injury in the postischemic liver in vivo. Transplantation. 74:681–688. 2002. View Article : Google Scholar

13 

Cook JA: Eicosanoids. Crit Care Med. 33 12 Suppl:S488–S491. 2005. View Article : Google Scholar

14 

Ito S, Ito Y, Katagiri H, Suzuki T, Hoka S, Yokomizo T, Shimizu T and Majima M: Leukotriene B4/leukotriene B4 receptor pathway is involved in hepatic microcirculatory dysfunction elicited by endotoxin. Shock. 30:87–91. 2008.

15 

Reddy RC, Chen GH, Tateda K, Tsai WC, Phare SM, Mancuso P, Peters-Golden M and Standiford TJ: Selective inhibition of COX-2 improves early survival in murine endotoxemia but not in bacterial peritonitis. Am J Physiol Lung Cell Mol Physiol. 281:L537–L543. 2001.

16 

Hsiao G, Lin KH, Chang Y, Chen TL, Tzu NH, Chou DS and Sheu JR: Protective mechanisms of inosine in platelet activation and cerebral ischemic damage. Arterioscler Thromb Vasc Biol. 25:1998–2004. 2005. View Article : Google Scholar

17 

Sun P, Zhang P, Wang PX, Zhu LH, Du Y, Tian S, Zhu X and Li H: Mindin deficiency protects the liver against ischemia/reperfusion injury. J Hepatol. 63:1198–1211. 2015. View Article : Google Scholar

18 

Hsu YP, Chen RJ, Fang JF, Lin BC, Huang TL, Cheng ML, Chiu DT and Tsay PK: Increased susceptibility to oxidant injury in hepatocytes from rats with intra-abdominal hypertension. J Trauma. 57:569–575. 2004. View Article : Google Scholar

19 

Chou DS, Hsiao G, Lai YA, Tsai YJ and Sheu JR: Baicalein induces proliferation inhibition in B16F10 melanoma cells by generating reactive oxygen species via 12-lipoxygenase. Free Radic Biol Med. 46:1197–1203. 2009. View Article : Google Scholar

20 

Chou DS, Hsiao G, Shen MY, Tsai YJ, Chen TF and Sheu JR: ESR spin trapping of a carbon-centered free radical from agonist-stimulated human platelets. Free Radic Biol Med. 39:237–248. 2005. View Article : Google Scholar

21 

Shvedova AA, Kisin ER, Murray AR, Mouithys-Mickalad A, Stadlerd K, Masond RP and Kadiisk M: ESR evidence for in vivo formation of free radicals in tissue of mice exposed to single-walled carbon nanotubes. Free Radic Biol Med. 73:154–165. 2014. View Article : Google Scholar

22 

Qian SY, Wang HP, Schafer FQ and Buettner GR: EPR detection of lipid-derived free radicals from PUFA, LDL, and cell oxidations. Free Radic Biol Med. 29:568–579. 2000. View Article : Google Scholar

23 

Aristatile B, Al-Assaf AH and Pugalendi KV: Carvacrol ameliorates the PPAR-A and cytochrome p450 expression on D-galactosamine induced hepatotoxicity rats. Afr J Tradit Complement Altern Med. 11:118–123. 2014. View Article : Google Scholar :

24 

Khandoga A, Biberthaler P, Messmer K and Krombach F: Platelet-endothelial cell interactions during hepatic ischemia-reperfusion in vivo: A systematic analysis. Microvasc Res. 65:71–77. 2003. View Article : Google Scholar

25 

Woolbright BL and Jaeschke H: Heme oxygenase-1 and platelets in hepatic ischemia reperfusion injury. J Gastroenterol Hepatol. 28:756–757. 2013. View Article : Google Scholar

26 

Kubo SH, Walter BA, John DH, Clark M and Cody RJ: Liver function abnormalities in chronic heart failure. Influence of systemic hemodynamics. Arch Intern Med. 147:1227–1230. 1987. View Article : Google Scholar

27 

Wu YH, Hu SQ, Liu J, Cao HC, Xu W, Li YJ and Li LJ: Nature and mechanisms of hepatocyte apoptosis induced by D-galactosamine/lipopolysaccharide challenge in mice. Int J Mol Med. 33:1498–1506. 2014. View Article : Google Scholar :

28 

Sumida Y, Niki E, Naito Y and Yoshikawa T: Involvement of free radicals and oxidative stress in NAFLD/NASH. Free Radic Res. 47:869–880. 2013. View Article : Google Scholar

29 

Yang YY, Liu H, Nam SW, Kunos G and Lee SS: Mechanisms of TNFalpha-induced cardiac dysfunction in cholestatic bile duct-ligated mice: Interaction between TNFalpha and endocannabinoids. J Hepatol. 53:298–306. 2010. View Article : Google Scholar :

30 

Maskrey BH, Bermúdez-Fajardo A, Morgan AH, Stewart-Jones E, Dioszeghy V, Taylor GW, Baker PR, Coles B, Coffey MJ, Kühn H and O'Donnell VB: Activated platelets and monocytes generate four hydroxyphosphatidylethanolamines via lipoxygenase. J Biol Chem. 282:20151–20163. 2007. View Article : Google Scholar

31 

Johnson EN, Brass LF and Funk CD: Increased platelet sensitivity to ADP in mice lacking platelet-type 12-lipoxygenase. Proc Natl Acad Sci USA. 95:3100–3105. 1998. View Article : Google Scholar :

32 

Chou DS, Lee JJ, Hsiao G, Hsieh CY, Tsai YJ, Chen TF and Sheu JR: Baicalein induction of hydroxyl radical formation via 12-lipoxygenase in human platelets: An ESR study. J Agric Food Chem. 55:649–655. 2007. View Article : Google Scholar

33 

Morteau O: Prostaglandins and inflammation: The cyclooxygenase controversy. Arch Immunol Ther Exp (Warsz). 48:473–480. 2000.

34 

Ko CH, Shen SC, Lin HY, Hou WC, Lee WR, Yang LL and Chen YC: Flavanones structure-related inhibition on TPA induced tumor promotion through suppression of extracellular signal-regulated protein kinases: Involvement of prostaglandin E2 in anti-promotive process. J Cell Physiol. 193:93–102. 2002. View Article : Google Scholar

35 

Hodnick WF, Milosavljević EB, Nelson JH and Pardini RS: Electrochemistry of flavonoids. Relationships between redox potentials, inhibition of mitochondrial respiration, and production of oxygen radicals by flavonoids. Biochem Pharmacol. 37:2607–2611. 1988. View Article : Google Scholar

36 

Bors W, Michel C and Stettmaier K: Electron paramagnetic resonance studies of radical species of proanthocyanidins and gallate esters. Arch Biochem Biophys. 374:347–355. 2000. View Article : Google Scholar

37 

You KM, Jong HG and Kim HP: Inhibition of cyclooxygenase/lipoxygenase from human platelets by polyhydroxylated/methoxylated flavonoids isolated from medicinal plants. Arch Pharm Res. 22:18–24. 1999. View Article : Google Scholar

38 

Greenley TL and Davies MJ: Radical production from peroxide and peracid tumour promoters: EPR spin trapping studies. Biochim Biophys Acta. 1157:23–31. 1993. View Article : Google Scholar

39 

Morgan ET: Regulation of cytochrome p450 by inflammatory mediators: Why and how? Drug Metab Dispos. 29:207–212. 2001.

40 

Tolando R, Zanovello A, Ferrara R, Iley JN and Manno M: Inactivation of rat liver cytochrome P450 (P450) by N,N-dimethylformamide and N,N-dimethylacetamide. Toxicol Lett. 124:101–111. 2001. View Article : Google Scholar

41 

van Golen RF, Stevens KM, Colarusso P, Jaeschke H and Heger M: Platelet aggregation but not activation and degranulation during the acute post-ischemic reperfusion phase in livers with no underlying disease. J Clin Transl Res. 1:107–115. 2015.

42 

Starlinger P, Assinger A, Haegele S, Wanek D, Zikeli S, Schauer D, Birner P, Fleischmann E, Gruenberger B, Brostjan C and Gruenberger T: Evidence for serotonin as a relevant inducer of liver regeneration after liver resection in humans. Hepatology. 60:257–266. 2014. View Article : Google Scholar

43 

Mende K, Reifart J, Rosentreter D, Manukyan D, Mayr D, Krombach F, Rentsch M and Khandoga A: Targeting platelet migration in the postischemic liver by blocking protease-activated receptor 4. Transplantation. 97:154–160. 2014. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wang CL, Yang PS, Tsao JT, Jayakumar T, Wang MJ, Sheu JR and Chou DS: Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study. Mol Med Rep 17: 2061-2069, 2018.
APA
Wang, C., Yang, P., Tsao, J., Jayakumar, T., Wang, M., Sheu, J., & Chou, D. (2018). Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study. Molecular Medicine Reports, 17, 2061-2069. https://doi.org/10.3892/mmr.2017.8058
MLA
Wang, C., Yang, P., Tsao, J., Jayakumar, T., Wang, M., Sheu, J., Chou, D."Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study". Molecular Medicine Reports 17.1 (2018): 2061-2069.
Chicago
Wang, C., Yang, P., Tsao, J., Jayakumar, T., Wang, M., Sheu, J., Chou, D."Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study". Molecular Medicine Reports 17, no. 1 (2018): 2061-2069. https://doi.org/10.3892/mmr.2017.8058
Copy and paste a formatted citation
x
Spandidos Publications style
Wang CL, Yang PS, Tsao JT, Jayakumar T, Wang MJ, Sheu JR and Chou DS: Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study. Mol Med Rep 17: 2061-2069, 2018.
APA
Wang, C., Yang, P., Tsao, J., Jayakumar, T., Wang, M., Sheu, J., & Chou, D. (2018). Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study. Molecular Medicine Reports, 17, 2061-2069. https://doi.org/10.3892/mmr.2017.8058
MLA
Wang, C., Yang, P., Tsao, J., Jayakumar, T., Wang, M., Sheu, J., Chou, D."Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study". Molecular Medicine Reports 17.1 (2018): 2061-2069.
Chicago
Wang, C., Yang, P., Tsao, J., Jayakumar, T., Wang, M., Sheu, J., Chou, D."Mechanism of free radical generation in platelets and primary hepatocytes: A novel electron spin resonance study". Molecular Medicine Reports 17, no. 1 (2018): 2061-2069. https://doi.org/10.3892/mmr.2017.8058
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