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Article

microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma

  • Authors:
    • Zheng Luo
    • Gang Wu
    • Daiyang Zhang
    • Jun Liu
    • Renguo Ran
  • View Affiliations / Copyright

    Affiliations: Department of Spinal Surgery, The Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, P.R. China, Department of Inspection, The Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, P.R. China
  • Pages: 2005-2011
    |
    Published online on: November 15, 2017
       https://doi.org/10.3892/mmr.2017.8079
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Abstract

Osteosarcoma (OS) is the leading cause of cancer‑associated mortality in adolescents and children. MicroRNAs (miRNAs) have critical roles in cancer, particularly in the initiation and progression of OS. Therefore, OS‑associated miRNAs should be identified for use as therapeutic targets for treatment of OS. This study aimed to investigate the expression pattern, potential roles and underlying mechanism of microRNA‑625 (miR‑625) in OS. miR‑625 was markedly downregulated in OS tissues and cell lines compared with that in associated adjacent non‑tumor tissues and human normal osteoblasts, respectively. The enforced expression of miR‑625 using miRNA mimics significantly reduced the proliferation and invasion of OS cells. Bioinformatics analysis and luciferase reporter assays indicated that miR‑625 targeted the 3'‑untranslated region of Yes‑associated protein 1 (YAP‑1). Furthermore, upregulation of miR‑625 reduced endogenous YAP1 expression at the mRNA and protein levels. The upregulated expression of YAP1 in OS tissues was inversely correlated with miR‑625 expression. YAP1 restoration using a recombinant plasmid rescued the miR‑625‑mediated tumor‑suppressive effects in OS cells. In conclusion, miR‑625 attenuated the cell proliferation and invasion of OS by suppressing YAP1. Thus, miR‑625 may be a potential target for OS therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Luo Z, Wu G, Zhang D, Liu J and Ran R: microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma. Mol Med Rep 17: 2005-2011, 2018.
APA
Luo, Z., Wu, G., Zhang, D., Liu, J., & Ran, R. (2018). microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma. Molecular Medicine Reports, 17, 2005-2011. https://doi.org/10.3892/mmr.2017.8079
MLA
Luo, Z., Wu, G., Zhang, D., Liu, J., Ran, R."microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma". Molecular Medicine Reports 17.1 (2018): 2005-2011.
Chicago
Luo, Z., Wu, G., Zhang, D., Liu, J., Ran, R."microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma". Molecular Medicine Reports 17, no. 1 (2018): 2005-2011. https://doi.org/10.3892/mmr.2017.8079
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Z, Wu G, Zhang D, Liu J and Ran R: microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma. Mol Med Rep 17: 2005-2011, 2018.
APA
Luo, Z., Wu, G., Zhang, D., Liu, J., & Ran, R. (2018). microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma. Molecular Medicine Reports, 17, 2005-2011. https://doi.org/10.3892/mmr.2017.8079
MLA
Luo, Z., Wu, G., Zhang, D., Liu, J., Ran, R."microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma". Molecular Medicine Reports 17.1 (2018): 2005-2011.
Chicago
Luo, Z., Wu, G., Zhang, D., Liu, J., Ran, R."microRNA‑625 targets Yes‑associated protein 1 to suppress cell proliferation and invasion of osteosarcoma". Molecular Medicine Reports 17, no. 1 (2018): 2005-2011. https://doi.org/10.3892/mmr.2017.8079
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