Cytochrome c is important in apoptosis of labial glands in primary Sjogren's syndrome

Kang,Yuanyuan; Sun,Yan; Zhang,Ying; Wang,Zhe

doi:10.3892/mmr.2017.8083

Cytochrome c is important in apoptosis of labial glands in primary Sjogren's syndrome

Authors:
- Yuanyuan Kang
- Yan Sun
- Ying Zhang
- Zhe Wang
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Affiliations: Department of Emergency and Oral Medicine, The School of Stomatology, China Medical University and Liaoning Institute of Dental Research and Liaoning Province Key Laboratory of Oral Diseases and Liaoning Province Translational Medicine Research Center of Oral Diseases, Shenyang, Liaoning 110002, P.R. China, Department of Pathology, The Second Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110004, P.R. China
Published online on: November 15, 2017 https://doi.org/10.3892/mmr.2017.8083
Pages: 1993-1997

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Abstract

The present study aimed to investigate the expression and effect of cytochrome c (Cytc) in patients with primary Sjogren's syndrome (pSS). In total, 35 newly diagnosed pSS patients and 35 healthy subjects were enrolled in the present study. The mRNA expression levels of Cytc were detected using reverse transcription‑polymerase chain reaction and RT‑quantitative PCR. The expression of the Cytc protein in labial salivary glands was detected by immunohistochemistry and was associated with the integral optical density (IOD) of clinical and laboratory variables. In addition, the content of Cytc in the cytoplasm and mitochondria were examined. The mRNA and protein expression levels of Cytc, and the content of Cytc in the cytoplasm of the pSS patients was increased significantly compared with the healthy controls (P<0.05). The content of Cytc in the mitochondria was significantly decreased compared with the healthy controls (P<0.05). The IOD of Cytc protein levels was positively correlated with immunoglobin G (r=0.8142, P<0.05) and erythrocyte sedimentation rate (r=0.7512, P<0.05). Cytc was upregulated in the pSS patients, indicating the potential role of Cytc in the pathogenesis and development of pSS. Further studies may facilitate the development of targeting this molecular pathway for the treatment of pSS.

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