Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells

  • Authors:
    • Dan Shao
    • Chunfang Wang
    • Yaping Sun
    • Lei Cui
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The First People's Hospital of Qingdao Economic and Technological Development Zone, Qingdao, Shandong 266555, P.R. China
    Copyright: © Shao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1537-1544
    |
    Published online on: November 15, 2017
       https://doi.org/10.3892/mmr.2017.8094
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to regulate the transformation of bone marrow mesenchymal stem cells (BMMSCs) to osteoblasts to promote bone formation and osseointegration surrounding oral implants. BMMSCs were cultured using the whole bone marrow adherence method. Cell surface markers were detected by flow cytometry, and multi‑lineage differentiation potential was detected by osteogenic and adipogenic tests. miR‑122‑modified cell sheets were prepared by non‑viral transfection and complexed with micro‑arc titanium oxide implants to construct a gene‑modified tissue‑engineered implant, with its surface morphology observed by scanning electron microscopy (SEM). In vitro osteogenic activity of the implant was determined by alkaline phosphatase (ALP), Sirius Red, alizarin red staining, polymerase chain reaction and western blot analysis. The BMMSCs were spindle‑ or triangular‑shaped. Surface markers, cluster of differentiation 29 (CD29), CD90 and CD105 were positively expressed, whereas blood cell markers CD34, CD45 and CD31 were negatively expressed. Osteogenic staining exhibited deposition of calcified nodules, while adipogenic staining demonstrated the formation of lipid droplets. miR‑122 modification significantly enhanced the in vitro osteogenic activity of the sheets. On day 3 of osteogenic induction, runt-related transcription factor 2, osterix, osteocalcin, collagen I, ALP and bone morphogenetic protein 2 expression levels of the experimental group were 2.0, 3.1, 4.6, 3.2, 10.5 and 4.5 times those of the blank control group, respectively. SEM imaging of the modified sheet demonstrated close adhesion and fitting between abundant cellular and extracellular matrices, and the porous surface of the implant. In vitro osteogenesis of the complex was promoted and accelerated. Thus, miR‑122 effectively promoted osteogenic differentiation of the BMMSC sheet. Therefore, it is feasible to construct gene‑modified tissue‑engineered implants by complexing miR‑122‑modified sheets with micro‑arc titanium oxide implants.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Masuda S and Shimizu T: Three-dimensional cardiac tissue fabrication based on cell sheet technology. Adv Drug Deliv Rev. 96:103–109. 2016. View Article : Google Scholar

2 

Clark AG and Vignjevic DM: Modes of cancer cell invasion and the role of the microenvironment. Curr Opin Cell Biol. 36:13–22. 2015. View Article : Google Scholar

3 

Chian KS, Leong MF and Kono K: Regenerative medicine for oesophageal reconstruction after cancer treatment. Lancet Oncol. 16:e84–e92. 2015. View Article : Google Scholar

4 

Westrate LM, Lee JE, Prinz WA and Voeltz GK: Form follows function: The importance of endoplasmic reticulum shape. Annu Rev Biochem. 84:791–811. 2015. View Article : Google Scholar

5 

Pocha SM and Montell DJ: Cellular and molecular mechanisms of single and collective cell migrations in Drosophila: Themes and variations. Annu Rev Genet. 48:295–318. 2014. View Article : Google Scholar

6 

Sugimura R: Bioengineering hematopoietic stem cell niche toward regenerative medicine. Adv Drug Deliv Rev. 99:212–220. 2016. View Article : Google Scholar

7 

Flor TB and Blom B: Pathogens use and abuse microRNAs to deceive the immune system. Int J Mol Sci. 17:5382016. View Article : Google Scholar :

8 

Hayes CN and Chayama K: MicroRNAs as biomarkers for liver disease and hepatocellular carcinoma. Int J Mol Sci. 17:2802016. View Article : Google Scholar :

9 

Huang JT, Liu SM, Ma H, Yang Y, Zhang X, Sun H, Zhang X, Xu J and Wang J: Systematic review and meta-analysis: Circulating miRNAs for diagnosis of hepatocellular carcinoma. J Cell Physiol. 231:328–335. 2016. View Article : Google Scholar

10 

Farooqi AA, Fayyaz S, Shatynska-Mytsyk I, Javed Z, Jabeen S, Yaylim I, Gasparri ML and Panici PB: Is miR-34a a well-equipped swordsman to conquer temple of molecular oncology? Chem Biol Drug Des. 87:321–334. 2016. View Article : Google Scholar

11 

Chmielewska AM, Rychłowska M, Król E, Solarz K and Bieńkowska-Szewczyk K: Novel methods of hepatitis C treatment and prevention. Postepy Hig Med Dosw (Online). 69:946–963. 2015. View Article : Google Scholar

12 

Stachowiak G, Zajac A, Nowak M, Stetkiewicz T and Wilczyński JR: Hemostatic disorders of the menopausal period: The role of microRNA. Prz Menopauzalny. 14:144–148. 2015.

13 

Casas-Agustench P, Iglesias-Gutiérrez E and Dávalos A: Mother's nutritional miRNA legacy: Nutrition during pregnancy and its possible implications to develop cardiometabolic disease in later life. Pharmacol Res. 100:322–334. 2015. View Article : Google Scholar

14 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

15 

Ivanovska IL, Shin JW, Swift J and Discher DE: Stem cell mechanobiology: Diverse lessons from bone marrow. Trends Cell Biol. 25:523–532. 2015. View Article : Google Scholar :

16 

Willeit P, Skroblin P, Kiechl S, Fernández-Hernando C and Mayr M: Liver microRNAs: Potential mediators and biomarkers for metabolic and cardiovascular disease? Eur Heart J. 37:3260–3266. 2016. View Article : Google Scholar :

17 

McGill MR and Jaeschke H: MicroRNAs as signaling mediators and biomarkers of drug- and chemical-induced liver injury. J Clin Med. 4:1063–1078. 2015. View Article : Google Scholar :

18 

Sedano CD and Sarnow P: Interaction of host cell microRNAs with the HCV RNA genome during infection of liver cells. Semin Liver Dis. 35:75–80. 2015. View Article : Google Scholar :

19 

Motavaf M, Safari S and Alavian SM: Targeting microRNA-122: Walking on cutting edge of hepatitis C virus infection therapy. Acta Virol. 58:301–308. 2014. View Article : Google Scholar

20 

He L, Tian DA, Li PY and He XX: Mouse models of liver cancer: Progress and recommendations. Oncotarget. 6:23306–23322. 2015. View Article : Google Scholar :

21 

Gibson NW: Engineered microRNA therapeutics. J R Coll Physicians Edinb. 44:196–200. 2014. View Article : Google Scholar

22 

Wilson JA and Sagan SM: Hepatitis C virus and human miR-122: Insights from the bench to the clinic. Curr Opin Virol. 7:11–18. 2014. View Article : Google Scholar

23 

Gupta P, Cairns MJ and Saksena NK: Regulation of gene expression by microRNA in HCV infection and HCV-mediated hepatocellular carcinoma. Virol J. 11:642014. View Article : Google Scholar :

24 

Baek J, Kang S and Min H: MicroRNA-targeting therapeutics for hepatitis C. Arch Pharm Res. 37:299–305. 2014. View Article : Google Scholar

25 

Qiu Z and Dai Y: Roadmap of miR-122-related clinical application from bench to bedside. Expert Opin Investig Drugs. 23:347–355. 2014. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shao D, Wang C, Sun Y and Cui L: Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells. Mol Med Rep 17: 1537-1544, 2018.
APA
Shao, D., Wang, C., Sun, Y., & Cui, L. (2018). Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells. Molecular Medicine Reports, 17, 1537-1544. https://doi.org/10.3892/mmr.2017.8094
MLA
Shao, D., Wang, C., Sun, Y., Cui, L."Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 17.1 (2018): 1537-1544.
Chicago
Shao, D., Wang, C., Sun, Y., Cui, L."Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 17, no. 1 (2018): 1537-1544. https://doi.org/10.3892/mmr.2017.8094
Copy and paste a formatted citation
x
Spandidos Publications style
Shao D, Wang C, Sun Y and Cui L: Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells. Mol Med Rep 17: 1537-1544, 2018.
APA
Shao, D., Wang, C., Sun, Y., & Cui, L. (2018). Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells. Molecular Medicine Reports, 17, 1537-1544. https://doi.org/10.3892/mmr.2017.8094
MLA
Shao, D., Wang, C., Sun, Y., Cui, L."Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 17.1 (2018): 1537-1544.
Chicago
Shao, D., Wang, C., Sun, Y., Cui, L."Effects of oral implants with miR‑122‑modified cell sheets on rat bone marrow mesenchymal stem cells". Molecular Medicine Reports 17, no. 1 (2018): 1537-1544. https://doi.org/10.3892/mmr.2017.8094
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team