Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2018 Volume 17 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Review Open Access

Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review)

  • Authors:
    • Stella Baliou
    • Maria Adamaki
    • Anthony M. Kyriakopoulos
    • Demetrios A. Spandidos
    • Michalis Panayiotidis
    • Ioannis Christodoulou
    • Vassilis Zoumpourlis
  • View Affiliations / Copyright

    Affiliations: National Hellenic Research Foundation, 11635 Athens, Greece, Nasco AD Biotechnology Laboratory, 18536 Pireus, Greece, Laboratory of Clinical Virology, Medical School, University of Crete, 71003 Heraklion, Greece, Department of Applied Sciences, Northumbria University, Newcastle Upon Tyne NE1 8ST, UK
    Copyright: © Baliou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1421-1427
    |
    Published online on: November 16, 2017
       https://doi.org/10.3892/mmr.2017.8099
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Even though the accrual of transcripts is implicated in distinct disease states, our knowledge regarding their functional role remains obscure. The CRISPR system has surged at the forefront of genome engineering tools in the field of RNA modulation. In the present review, we discuss some exciting applications of the CRISPR system, including the manipulation of RNA sequences, the visualization of chromosomal loci in living cells and the modulation of transcription. The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9). In the present review, the CRISPR system is presented as an eminent tool for the meticulous analysis of gene regulation, loci mapping and complex pathways.
View Figures

Figure 1

Figure 2

View References

1 

Wright AV, Nuñez JK and Doudna JA: Biology and applications of CRISPR systems: Harnessing nature's toolbox for genome engineering. Cell. 164:29–44. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Wiedenheft B, Sternberg SH and Doudna JA: RNA-guided genetic silencing systems in bacteria and archaea. Nature. 482:331–338. 2012. View Article : Google Scholar : PubMed/NCBI

3 

van der Oost J, Westra ER, Jackson RN and Wiedenheft B: Unravelling the structural and mechanistic basis of CRISPR-cas systems. Nat Rev Microbiol. 12:479–492. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Mougiakos I, Bosma EF, de Vos WM, van Kranenburg R and van der Oost J: Next generation prokaryotic engineering: The CRISPR-cas toolkit. Trends Biotechnol. 34:575–587. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Bolotin A, Quinquis B, Sorokin A and Ehrlich SD: Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin. Microbiology. 151:2551–2561. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Westra ER, Semenova E, Datsenko KA, Jackson RN, Wiedenheft B, Severinov K and Brouns SJ: Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. PLoS Genet. 9:e10037422013. View Article : Google Scholar : PubMed/NCBI

7 

Mojica FJ, Díez-Villaseñor C, García-Martínez J and Almendros C: Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology. 155:733–740. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Jackson AL, Bartz SR, Schelter J, Kobayashi SV, Burchard J, Mao M, Li B, Cavet G and Linsley PS: Expression profiling reveals off-target gene regulation by RNAi. Nat Biotechnol. 21:635–637. 2003. View Article : Google Scholar : PubMed/NCBI

9 

Marine S, Bahl A, Ferrer M and Buehler E: Common seed analysis to identify off-target effects in siRNA screens. J Biomol Screen. 17:370–378. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Moore JD: The impact of CRISPR-Cas9 on target identification and validation. Drug Discov Today. 20:450–457. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA and Zhang F: Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 8:2281–2308. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Kuscu C, Arslan S, Singh R, Thorpe J and Adli M: Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease. Nat Biotechnol. 32:677–683. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Fu Y, Sander JD, Reyon D, Cascio VM and Joung JK: Improving CRISPR-cas nuclease specificity using truncated guide RNAs. Nat Biotechnol. 32:279–284. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP and Lim WA: Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression. Cell. 152:1173–1183. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Boettcher M and McManus MT: Choosing the right tool for the job: RNAi, TALEN, or CRISPR. Mol Cell. 58:575–585. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Lawhorn IE, Ferreira JP and Wang CL: Evaluation of sgRNA target sites for CRISPR-mediated repression of TP53. PLoS One. 9:e1132322014. View Article : Google Scholar : PubMed/NCBI

17 

Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, et al: CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes. Cell. 154:442–451. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Cong L, Platt RJ, Scott DA, Church GM and Zhang F: Optical control of mammalian endogenous transcription and epigenetic states. Nature. 500:472–476. 2013.PubMed/NCBI

19 

Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, et al: CRISPR interference efficiently induces specific and reversible gene silencing in human iPSCs. Cell Stem Cell. 18:541–553. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Larson MH, Gilbert LA, Wang X, Lim WA, Weissman JS and Qi LS: CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Nat Protoc. 8:2180–2196. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Maeder ML, Linder SJ, Cascio VM, Fu Y, Ho QH and Joung JK: CRISPR RNA-guided activation of endogenous human genes. Nat Methods. 10:977–979. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang L and Church GM: CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering. Nat Biotechnol. 31:833–838. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, et al: RNA-guided gene activation by CRISPR-Cas9-based transcription factors. Nat Methods. 10:973–976. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS and Vale RD: A protein-tagging system for signal amplification in gene expression and fluorescence imaging. Cell. 159:635–646. 2014. View Article : Google Scholar : PubMed/NCBI

25 

La Russa MF and Qi LS: The new state of the art: Cas9 for gene activation and repression. Mol Cell Biol. 35:3800–3809. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, et al: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 517:583–588. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle MPR, Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, et al: Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 12:326–328. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M, Tsai JC, Weissman JS, Dueber JE, Qi LS and Lim WA: Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds. Cell. 160:339–350. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Braun CJ, Bruno PM, Horlbeck MA, Gilbert LA, Weissman JS and Hemann MT: Versatile in vivo regulation of tumor phenotypes by dCas9-mediated transcriptional perturbation. Proc Natl Acad Sci USA. 113:E3892–E3900. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Mansour MR, Abraham BJ, Anders L, Berezovskaya A, Gutierrez A, Durbin AD, Etchin J, Lawton L, Sallan SE, Silverman LB, et al: Oncogene regulation. An oncogenic super-enhancer formed through somatic mutation of a noncoding intergenic element. Science. 346:1373–1377. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Inoue F, Kircher M, Martin B, Cooper GM, Witten DM, McManus MT, Ahituv N and Shendure J: A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity. Genome Res. 27:38–52. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Xie S, Duan J, Li B, Zhou P and Hon GC: Multiplexed engineering and analysis of combinatorial enhancer activity in single cells. Molecular cell. 66:285–99. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Chiba K, Johnson JZ, Vogan JM, Wagner T, Boyle JM and Hockemeyer D: Cancer-associated TERT promoter mutations abrogate telomerase silencing. eLife. 4:42015. View Article : Google Scholar

34 

Polstein LR and Gersbach CA: A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol. 11:198–200. 2015. View Article : Google Scholar : PubMed/NCBI

35 

Hemphill J, Borchardt EK, Brown K, Asokan A and Deiters A: Optical Control of CRISPR/Cas9 gene editing. J Am Chem Soc. 137:5642–5645. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Nihongaki Y, Kawano F, Nakajima T and Sato M: Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol. 33:755–760. 2015. View Article : Google Scholar : PubMed/NCBI

37 

Liu KI, Ramli MN, Woo CW, Wang Y, Zhao T, Zhang X, Yim GR, Chong BY, Gowher A, Chua MZ, et al: A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing. Nat Chem Biol. 12:980–987. 2016. View Article : Google Scholar : PubMed/NCBI

38 

Zetsche B, Volz SE and Zhang F: A split-Cas9 architecture for inducible genome editing and transcription modulation. Nat Biotechnol. 33:139–142. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, et al: Genome-scale CRISPR-mediated control of gene repression and activation. Cell. 159:647–661. 2014. View Article : Google Scholar : PubMed/NCBI

40 

Haemmerle M and Gutschner T: Long non-coding RNAs in cancer and development: Where do we go from here? Int J Mol Sci. 16:1395–1405. 2015. View Article : Google Scholar : PubMed/NCBI

41 

Hale CR, Zhao P, Olson S, Duff MO, Graveley BR, Wells L, Terns RM and Terns MP: RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell. 139:945–956. 2009. View Article : Google Scholar : PubMed/NCBI

42 

Gutschner T: Silencing long noncoding RNAs with genome-editing tools. Methods Mol Biol. 1239:241–250. 2015. View Article : Google Scholar : PubMed/NCBI

43 

Sauvageau M, Goff LA, Lodato S, Bonev B, Groff AF, Gerhardinger C, Sanchez-Gomez DB, Hacisuleyman E, Li E, Spence M, et al: Multiple knockout mouse models reveal lincRNAs are required for life and brain development. eLife. 2:e017492013. View Article : Google Scholar : PubMed/NCBI

44 

Yin Y, Yan P, Lu J, Song G, Zhu Y, Li Z, Zhao Y, Shen B, Huang X, Zhu H, et al: Opposing roles for the lncRNA haunt and its genomic locus in regulating HOXA gene activation during embryonic stem cell differentiation. Cell Stem Cell. 16:504–516. 2015. View Article : Google Scholar : PubMed/NCBI

45 

Xiao A, Wang Z, Hu Y, Wu Y, Luo Z, Yang Z, Zu Y, Li W, Huang P, Tong X, et al: Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish. Nucleic Acids Res. 41:e1412013. View Article : Google Scholar : PubMed/NCBI

46 

Li X, Chen W, Zeng W, Wan C, Duan S and Jiang S: microRNA-137 promotes apoptosis in ovarian cancer cells via the regulation of XIAP. Br J Cancer. 116:66–76. 2017. View Article : Google Scholar : PubMed/NCBI

47 

Zhen S, Hua L, Liu YH, Sun XM, Jiang MM, Chen W, Zhao L and Li X: Inhibition of long non-coding RNA UCA1 by CRISPR/Cas9 attenuated malignant phenotypes of bladder cancer. Oncotarget. 8:9634–9646. 2017.PubMed/NCBI

48 

Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T, Argani P, Rinn JL, et al: Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 464:1071–1076. 2010. View Article : Google Scholar : PubMed/NCBI

49 

Singh R, Gupta SC, Peng WX, Zhou N, Pochampally R, Atfi A, Watabe K, Lu Z and Mo YY: Regulation of alternative splicing of Bcl-x by BC200 contributes to breast cancer pathogenesis. Cell Death Dis. 7:e22622016. View Article : Google Scholar : PubMed/NCBI

50 

Yin Y, Zhong J, Li SW, Li JZ, Zhou M, Chen Y, Sang Y and Liu L: TRIM11, a direct target of miR-24-3p, promotes cell proliferation and inhibits apoptosis in colon cancer. Oncotarget. 7:86755–86765. 2016.PubMed/NCBI

51 

Cheng J, Roden CA, Pan W, Zhu S, Baccei A, Pan X, Jiang T, Kluger Y, Weissman SM, Guo S, et al: A molecular chipper technology for CRISPR sgRNA library generation and functional mapping of noncoding regions. Nat Commun. 7:111782016. View Article : Google Scholar : PubMed/NCBI

52 

Shechner DM, Hacisuleyman E, Younger ST and Rinn JL: Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display. Nat Methods. 12:664–670. 2015. View Article : Google Scholar : PubMed/NCBI

53 

Plummer RJ, Guo Y and Peng Y: A CRISPR reimagining: New twists and turns of CRISPR beyond the genome-engineering revolution. J Cell Biochem. 2017, https://doi.org/10.1002/jcb.26406 View Article : Google Scholar

54 

Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, Klasić M and Zoldoš V: Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 44:5615–5628. 2016. View Article : Google Scholar : PubMed/NCBI

55 

Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L and Lombardo A: Inheritable silencing of endogenous genes by hit-and-run targeted epigenetic editing. Cell. 167:219–232. 2016. View Article : Google Scholar : PubMed/NCBI

56 

Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA and Jaenisch R: Editing DNA methylation in the mammalian genome. Cell. 167:233–247. 2016. View Article : Google Scholar : PubMed/NCBI

57 

Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE and Gersbach CA: Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Nat Biotechnol. 33:510–517. 2015. View Article : Google Scholar : PubMed/NCBI

58 

Pineda M, Moghadam F, Ebrahimkhani MR and Kiani S: Engineered CRISPR systems for next generation gene therapies. ACS Synth Biol. 6:1614–1626. 2017. View Article : Google Scholar : PubMed/NCBI

59 

Beliveau BJ, Joyce EF, Apostolopoulos N, Yilmaz F, Fonseka CY, McCole RB, Chang Y, Li JB, Senaratne TN, Williams BR, et al: Versatile design and synthesis platform for visualizing genomes with oligopaint FISH probes. Proc Natl Acad Sci USA. 109:21301–21306. 2012. View Article : Google Scholar : PubMed/NCBI

60 

Heun P, Laroche T, Shimada K, Furrer P and Gasser SM: Chromosome dynamics in the yeast interphase nucleus. Science. 294:2181–2186. 2001. View Article : Google Scholar : PubMed/NCBI

61 

Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS and Huang B: Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell. 155:1479–1491. 2013. View Article : Google Scholar : PubMed/NCBI

62 

Miyanari Y, Ziegler-Birling C and Torres-Padilla ME: Live visualization of chromatin dynamics with fluorescent TALEs. Nat Struct Mol Biol. 20:1321–1324. 2013. View Article : Google Scholar : PubMed/NCBI

63 

Roukos V, Voss TC, Schmidt CK, Lee S, Wangsa D and Misteli T: Spatial dynamics of chromosome translocations in living cells. Science. 341:660–664. 2013. View Article : Google Scholar : PubMed/NCBI

64 

van Steensel B and Dekker J: Genomics tools for unraveling chromosome architecture. Nat Biotechnol. 28:1089–1095. 2010. View Article : Google Scholar : PubMed/NCBI

65 

Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA and Yeo GW: Programmable RNA tracking in live cells with CRISPR/Cas9. Cell. 165:488–496. 2016. View Article : Google Scholar : PubMed/NCBI

66 

Wang S, Su JH, Zhang F and Zhuang X: An RNA-aptamer-based two-color CRISPR labeling system. Sci Rep. 6:268572016. View Article : Google Scholar : PubMed/NCBI

67 

Jaitin DA, Weiner A, Yofe I, Lara-Astiaso D, Keren-Shaul H, David E, Salame TM, Tanay A, van Oudenaarden A and Amit I: Dissecting immune circuits by linking CRISPR-pooled screens with single-Cell RNA-Seq. Cell. 167:1883–1896. 2016. View Article : Google Scholar : PubMed/NCBI

68 

Guernet A, Mungamuri SK, Cartier D, Sachidanandam R, Jayaprakash A, Adriouch S, Vezain M, Charbonnier F, Rohkin G, Coutant S, et al: CRISPR-barcoding for intratumor genetic heterogeneity modeling and functional analysis of oncogenic driver mutations. Mol Cell. 63:526–538. 2016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Baliou S, Adamaki M, Kyriakopoulos AM, Spandidos DA, Panayiotidis M, Christodoulou I and Zoumpourlis V: Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review). Mol Med Rep 17: 1421-1427, 2018.
APA
Baliou, S., Adamaki, M., Kyriakopoulos, A.M., Spandidos, D.A., Panayiotidis, M., Christodoulou, I., & Zoumpourlis, V. (2018). Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review). Molecular Medicine Reports, 17, 1421-1427. https://doi.org/10.3892/mmr.2017.8099
MLA
Baliou, S., Adamaki, M., Kyriakopoulos, A. M., Spandidos, D. A., Panayiotidis, M., Christodoulou, I., Zoumpourlis, V."Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review)". Molecular Medicine Reports 17.1 (2018): 1421-1427.
Chicago
Baliou, S., Adamaki, M., Kyriakopoulos, A. M., Spandidos, D. A., Panayiotidis, M., Christodoulou, I., Zoumpourlis, V."Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review)". Molecular Medicine Reports 17, no. 1 (2018): 1421-1427. https://doi.org/10.3892/mmr.2017.8099
Copy and paste a formatted citation
x
Spandidos Publications style
Baliou S, Adamaki M, Kyriakopoulos AM, Spandidos DA, Panayiotidis M, Christodoulou I and Zoumpourlis V: Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review). Mol Med Rep 17: 1421-1427, 2018.
APA
Baliou, S., Adamaki, M., Kyriakopoulos, A.M., Spandidos, D.A., Panayiotidis, M., Christodoulou, I., & Zoumpourlis, V. (2018). Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review). Molecular Medicine Reports, 17, 1421-1427. https://doi.org/10.3892/mmr.2017.8099
MLA
Baliou, S., Adamaki, M., Kyriakopoulos, A. M., Spandidos, D. A., Panayiotidis, M., Christodoulou, I., Zoumpourlis, V."Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review)". Molecular Medicine Reports 17.1 (2018): 1421-1427.
Chicago
Baliou, S., Adamaki, M., Kyriakopoulos, A. M., Spandidos, D. A., Panayiotidis, M., Christodoulou, I., Zoumpourlis, V."Role of the CRISPR system in controlling gene transcription and monitoring cell fate (Review)". Molecular Medicine Reports 17, no. 1 (2018): 1421-1427. https://doi.org/10.3892/mmr.2017.8099
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team