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Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity

  • Authors:
    • Yongtao Yang
    • Changmin Qu
    • Shuwen Liang
    • Gang Wang
    • Haolun Han
    • Na Chen
    • Xiaoying Wang
    • Zhiwen Luo
    • Changqing Zhong
    • Yan Chen
    • Lianyong Li
    • Wei Wu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The 306th Hospital of PLA, Beijing 100101, P.R. China, Department of Otorhinolaryngology, The 306th Hospital of PLA, Beijing 100101, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2313-2320
    |
    Published online on: November 20, 2017
       https://doi.org/10.3892/mmr.2017.8109
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Abstract

Microgravity can affect many aspects of intestinal homeostasis, leading to an increased risk of colitis. Estrogen, the most frequently affected hormone when under simulated microgravity, regulates the permeability of the colonic mucosa barrier. The associations between alterations in intestinal microbiota and increased susceptibility under microgravity have not been thoroughly elucidated. The aim of the present study was to evaluate the changes in intestinal microbiota under simulated microgravity and to investigate the protective effect of estrogen against those changes. The hindlimb unweighting (HU) model was used to simulate microgravity in rats. Estrogen was administered via intramuscular injection. Amplicons of the V3 variable regions of bacterial 16S rDNA were analyzed using denaturing gradient gel electrophoresis (DGGE), cloning and sequencing. Several specific bacterial groups were assayed using quantitative‑polymerase chain reaction. Bacterial translocation was evaluated by detecting serum lipopolysaccharide (LPS) and LPS binding protein (LBP) levels. DGGE profiles generated by universal primers revealed minor, though specific, changes in bacterial communities under simulated microgravity, particularly the band matching the sequence of Escherichia coli (E. coli). The quantification of 16S RNA revealed increased numbers of Bacteroides fragilis, E. coli and Fusobacterium nucleatum; however, Bifidobacteria longum significantly decreased under microgravity. Estrogen inhibited the overgrowth of E. coli, and decreased the levels of LBS and LBP under simulated microgravity. These results demonstrated that simulated microgravity alters the intestinal microflora and may contribute to bacterial translocation in the gut mucosa. The data also suggested that further investigations evaluating the administration of estrogen to protect against microgravity‑associated diseases may be required.
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1 

Saei AA and Barzegari A: The microbiome: The forgotten organ of the astronaut's body-probiotics beyond terrestrial limits. Future Microbiol. 7:1037–1046. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Cervantes JL and Hong BY: Dysbiosis and immune dysregulation in outer space. Int Rev Immunol. 35:67–82. 2016.PubMed/NCBI

3 

Tou JC, Grindeland RE and Wade CE: Effects of diet and exposure to hindlimb suspension on estrous cycling in Sprague-Dawley rats. Am J Physiol Endocrinol Metab. 286:E425–E433. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Neuman H, Debelius JW, Knight R and Koren O: Microbial endocrinology: The interplay between the microbiota and the endocrine system. FEMS Microbiol Rev. 39:509–521. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Li P, Shi J, Zhang P, Wang K, Li J, Liu H, Zhou Y, Xu X, Hao J, Sun X, et al: Simulated microgravity disrupts intestinal homeostasis and increases colitis susceptibility. FASEB J. 29:3263–3273. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Ritchie LE, Taddeo SS, Weeks BR, Lima F, Bloomfield SA, Azcarate-Peril MA, Zwart SR, Smith SM and Turner ND: Space environmental factor impacts upon murine colon microbiota and mucosal homeostasis. PLoS One. 10:e01257922015. View Article : Google Scholar : PubMed/NCBI

7 

Zhou Y, Ni H, Li M, Sanzari JK, Diffenderfer ES, Lin L, Kennedy AR and Weissman D: Effect of solar particle event radiation and hindlimb suspension on gastrointestinal tract bacterial translocation and immune activation. PLoS One. 7:e443292012. View Article : Google Scholar : PubMed/NCBI

8 

Looijer-van Langen M, Hotte N, Dieleman LA, Albert E, Mulder C and Madsen KL: Estrogen receptor-β signaling modulates epithelial barrier function. Am J Physiol Gastrointest Liver Physiol. 300:G621–G626. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Menon R, Watson SE, Thomas LN, Allred CD, Dabney A, Azcarate-Peril MA and Sturino JM: Diet complexity and estrogen receptor β status affect the composition of the murine intestinal microbiota. Appl Environ Microbiol. 79:5763–5773. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Wang MH and Achkar JP: Gene-environment interactions in inflammatory bowel disease pathogenesis. Curr Opin Gastroenterol. 31:277–282. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Walter J and Ley R: The human gut microbiome: Ecology and recent evolutionary changes. Annu Rev Microbiol. 65:411–429. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Benson AK, Kelly SA, Legge R, Ma F, Low SJ, Kim J, Zhang M, Oh PL, Nehrenberg D, Hua K, et al: Individuality in gut microbiota composition is a complex polygenic trait shaped by multiple environmental and host genetic factors. Proc Natl Acad Sci USA. 107:pp. 18933–18938. 2010; View Article : Google Scholar : PubMed/NCBI

13 

Du F, Ding Y, Zou J, Li Z, Tian J, She R, Wang D, Wang H, Lv D and Chang L: Morphology and molecular mechanisms of hepatic injury in rats under simulated weightlessness and the protective effects of resistance training. PLoS One. 10:e01270472015. View Article : Google Scholar : PubMed/NCBI

14 

Zhang R, Ran HH, Cai LL, Zhu L, Sun JF, Peng L, Liu XJ, Zhang LN, Fang Z, Fan YY and Cui G: Simulated microgravity-induced mitochondrial dysfunction in rat cerebral arteries. FASEB J. 28:2715–2724. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Su Y, Yao W, Perez-Gutierrez ON, Smidt H and Zhu WY: 16S ribosomal RNA-based methods to monitor changes in the hindgut bacterial community of piglets after oral administration of Lactobacillus sobrius S1. Anaerobe. 14:78–86. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Breton J, Massart S, Vandamme P, De Brandt E, Pot B and Foligné B: Ecotoxicology inside the gut: Tmpact of heavy metals on the mouse microbiome. BMC Pharmacol Toxicol. 14:622013. View Article : Google Scholar : PubMed/NCBI

17 

Lozupone CA and Knight R: Species divergence and the measurement of microbial diversity. FEMS Microbiol Rev. 32:557–578. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Xu M, Wang B, Fu Y, Chen Y, Yang F, Lu H, Chen Y, Xu J and Li L: Changes of fecal Bifidobacterium species in adult patients with hepatitis B virus-induced chronic liver disease. Microb Ecol. 63:304–313. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Chen Y, Yang F, Lu H, Wang B, Chen Y, Lei D, Wang Y, Zhu B and Li L: Characterization of fecal microbial communities in patients with liver cirrhosis. Hepatology. 54:562–572. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Du Plessis J, Vanheel H, Janssen CE, Roos L, Slavik T, Stivaktas PI, Nieuwoudt M, van Wyk SG, Vieira W, Pretorius E, et al: Activated intestinal macrophages in patients with cirrhosis release NO and IL-6 that may disrupt intestinal barrier function. J Hepatol. 58:1125–1132. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Barman M, Unold D, Shifley K, Amir E, Hung K, Bos N and Salzman N: Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract. Infect Immun. 76:907–915. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Lynch SV, Brodie EL and Matin A: Role and regulation of sigma S in general resistance conferred by low-shear simulated microgravity in Escherichia coli. J Bacteriol. 186:8207–8212. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Bailey MT, Dowd SE, Galley JD, Hufnagle AR, Allen RG and Lyte M: Exposure to a social stressor alters the structure of the intestinal microbiota: Implications for stressor-induced immunomodulation. Brain Behav Immun. 25:397–407. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Rosenzweig JA, Abogunde O, Thomas K, Lawal A, Nguyen YU, Sodipe A and Jejelowo O: Spaceflight and modeled microgravity effects on microbial growth and virulence. Appl Microbiol Biotechnol. 85:885–891. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Allen CA, Niesel DW and Torres AG: The effects of low-shear stress on Adherent-invasive Escherichia coli. Environ Microbiol. 10:1512–1525. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Zhang YJ, Li S, Gan RY, Zhou T, Xu DP and Li HB: Impacts of gut bacteria on human health and diseases. Int J Mol Sci. 16:7493–7519. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Gombošová L, Lazúrová I, Zakuciová M, Curová K, Kmeťová M, Petrášová D and Siegfried L: Genes of intestinal Escherichia coli and their relation to the inflammatory activity in patients with ulcerative colitis and Crohn's disease. Folia Microbiol (Praha). 56:367–372. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Arunasri K, Adil M, Venu Charan K, Suvro C, Himabindu Reddy S and Shivaji S: Effect of simulated microgravity on E. Coli K12 MG1655 growth and gene expression. PLoS One. 8:e578602013. View Article : Google Scholar : PubMed/NCBI

29 

Rubtsova K, Marrack P and Rubtsov AV: Sexual dimorphism in autoimmunity. J Clin Invest. 125:2187–2193. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Yurkovetskiy L, Burrows M, Khan AA, Graham L, Volchkov P, Becker L, Antonopoulos D, Umesaki Y and Chervonsky AV: Gender bias in autoimmunity is influenced by microbiota. Immunity. 39:400–412. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Xavier RJ and Podolsky DK: Unravelling the pathogenesis of inflammatory bowel disease. Nature. 448:427–434. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Bashir A, Miskeen AY, Bhat A, Fazili KM and Ganai BA: Fusobacterium nucleatum: An emerging bug in colorectal tumorigenesis. Eur J Cancer Prev. 24:373–385. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Sears CL, Geis AL and Housseau F: Bacteroides fragilis subverts mucosal biology: From symbiont to colon carcinogenesis. J Clin Invest. 124:4166–4172. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Castellarin M, Warren RL, Freeman JD, Dreolini L, Krzywinski M, Strauss J, Barnes R, Watson P, Allen-Vercoe E, Moore RA and Holt RA: Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma. Genome Res. 22:299–306. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Mermel LA: Infection prevention and control during prolonged human space travel. Clin Infect Dis. 56:123–130. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang Y, Qu C, Liang S, Wang G, Han H, Chen N, Wang X, Luo Z, Zhong C, Chen Y, Chen Y, et al: Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity. Mol Med Rep 17: 2313-2320, 2018.
APA
Yang, Y., Qu, C., Liang, S., Wang, G., Han, H., Chen, N. ... Wu, W. (2018). Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity. Molecular Medicine Reports, 17, 2313-2320. https://doi.org/10.3892/mmr.2017.8109
MLA
Yang, Y., Qu, C., Liang, S., Wang, G., Han, H., Chen, N., Wang, X., Luo, Z., Zhong, C., Chen, Y., Li, L., Wu, W."Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity". Molecular Medicine Reports 17.2 (2018): 2313-2320.
Chicago
Yang, Y., Qu, C., Liang, S., Wang, G., Han, H., Chen, N., Wang, X., Luo, Z., Zhong, C., Chen, Y., Li, L., Wu, W."Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity". Molecular Medicine Reports 17, no. 2 (2018): 2313-2320. https://doi.org/10.3892/mmr.2017.8109
Copy and paste a formatted citation
x
Spandidos Publications style
Yang Y, Qu C, Liang S, Wang G, Han H, Chen N, Wang X, Luo Z, Zhong C, Chen Y, Chen Y, et al: Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity. Mol Med Rep 17: 2313-2320, 2018.
APA
Yang, Y., Qu, C., Liang, S., Wang, G., Han, H., Chen, N. ... Wu, W. (2018). Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity. Molecular Medicine Reports, 17, 2313-2320. https://doi.org/10.3892/mmr.2017.8109
MLA
Yang, Y., Qu, C., Liang, S., Wang, G., Han, H., Chen, N., Wang, X., Luo, Z., Zhong, C., Chen, Y., Li, L., Wu, W."Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity". Molecular Medicine Reports 17.2 (2018): 2313-2320.
Chicago
Yang, Y., Qu, C., Liang, S., Wang, G., Han, H., Chen, N., Wang, X., Luo, Z., Zhong, C., Chen, Y., Li, L., Wu, W."Estrogen inhibits the overgrowth of Escherichia coli in the rat intestine under simulated microgravity". Molecular Medicine Reports 17, no. 2 (2018): 2313-2320. https://doi.org/10.3892/mmr.2017.8109
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