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Article Open Access

Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression

  • Authors:
    • Wanting Liu
    • Chaowu Liu
    • Li Zhang
    • Xiaofei Xie
    • Xiaoqiong Gu
    • Chuanlan Sang
    • Mingguo Xu
    • Weijun Xu
    • Hongling Jia
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou, Guangdong 510632, P.R. China, Department of Pediatric Cardiology, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510120, P.R. China, Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Department of Pediatric Cardiology, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China, Information Center, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2402-2414
    |
    Published online on: November 20, 2017
       https://doi.org/10.3892/mmr.2017.8111
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Abstract

Kawasaki disease (KD) is an acquired cardiac disease with a high incidence that affects children. KD has various complications, including coronary artery dilation (CAD) and coronary artery aneurysms (CAA). The identification of differentially expressed proteins and the underlying mechanisms may be the key to understanding differences between these KD complications. In the present study, isobaric tags for relative and absolute quantitation were used to identify variations in serum proteins between KD patients with CAD and CAA. In total, 87 (37 upregulated and 50 downregulated) and 65 (33 upregulated and 32 downregulated) significantly differentially‑expressed proteins were identified in comparisons between control samples (healthy individuals) and those obtained from patients with KD and with CAD or CAA. Investigation into the underlying biological process revealed that variations between the two complications were associated with the wound healing response, as well as lipoprotein‑ and cholesterol‑associated processes. Important proteins involved in the formation of the wound healing signaling network were identified via enriched biological processes and pathway analysis using ClueGo and ReactomeFIViz software. In the present study, 5 significantly differentially‑expressed proteins, including mannose binding lectin 2 (MBL2), complement factor H (CFH), kininogen 1 (KNG1), serpin family C member 1 (SERPINC1) and fibronectin 1 (FN1), were selected and confirmed by western blotting. Analysis indicated that these proteins were associated to immunity, inflammation and metabolism, serving a key role within each module, which has never been reported previously. The present study proposed that MBL2, CFH, KNG1, SERPINC1 and FN1 may be a potentially excellent indicator group for distinguishing the two major KD complications, CAD and CAA.
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Copy and paste a formatted citation
Spandidos Publications style
Liu W, Liu C, Zhang L, Xie X, Gu X, Sang C, Xu M, Xu W and Jia H: Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression. Mol Med Rep 17: 2402-2414, 2018.
APA
Liu, W., Liu, C., Zhang, L., Xie, X., Gu, X., Sang, C. ... Jia, H. (2018). Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression. Molecular Medicine Reports, 17, 2402-2414. https://doi.org/10.3892/mmr.2017.8111
MLA
Liu, W., Liu, C., Zhang, L., Xie, X., Gu, X., Sang, C., Xu, M., Xu, W., Jia, H."Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression". Molecular Medicine Reports 17.2 (2018): 2402-2414.
Chicago
Liu, W., Liu, C., Zhang, L., Xie, X., Gu, X., Sang, C., Xu, M., Xu, W., Jia, H."Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression". Molecular Medicine Reports 17, no. 2 (2018): 2402-2414. https://doi.org/10.3892/mmr.2017.8111
Copy and paste a formatted citation
x
Spandidos Publications style
Liu W, Liu C, Zhang L, Xie X, Gu X, Sang C, Xu M, Xu W and Jia H: Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression. Mol Med Rep 17: 2402-2414, 2018.
APA
Liu, W., Liu, C., Zhang, L., Xie, X., Gu, X., Sang, C. ... Jia, H. (2018). Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression. Molecular Medicine Reports, 17, 2402-2414. https://doi.org/10.3892/mmr.2017.8111
MLA
Liu, W., Liu, C., Zhang, L., Xie, X., Gu, X., Sang, C., Xu, M., Xu, W., Jia, H."Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression". Molecular Medicine Reports 17.2 (2018): 2402-2414.
Chicago
Liu, W., Liu, C., Zhang, L., Xie, X., Gu, X., Sang, C., Xu, M., Xu, W., Jia, H."Molecular basis of coronary artery dilation and aneurysms in patients with Kawasaki disease based on differential protein expression". Molecular Medicine Reports 17, no. 2 (2018): 2402-2414. https://doi.org/10.3892/mmr.2017.8111
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