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Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway

  • Authors:
    • Lei Jing
    • Xue‑Wen Jia
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, Ningbo First Hospital, Ningbo, Zhejiang 315000, P.R. China
    Copyright: © Jing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2415-2421
    |
    Published online on: November 21, 2017
       https://doi.org/10.3892/mmr.2017.8128
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Abstract

Palmitate (PA) has been identified to induce cell apoptosis in osteoblasts. The c‑Jun NH2‑teminal kinase (JNK) signaling pathway and endoplasmic reticulum stress (ERS) were found to be important contributors. Therefore, natural or synthetic agents may antagonize PA‑induced apoptosis in osteoblasts, and demonstrate the potential application to reverse osteoporosis. The present study demonstrated that the Lycium barbarum polysaccharide (LBP) is as a major active ingredient of Lycium barbarum and that it can reduce the fatty acid toxicity of PA. Furthermore, this study attempted to elucidate the underlying molecular mechanisms of LBP. Firstly, it was demonstrated via a Cell Counting Kit‑8 assay, that LBP could significantly increase the viability of MC3T3‑E1 cells in a dose‑dependent manner. Flow cytometric analysis indicated that LBP inhibits PA‑induced apoptosis in osteoblastic cells. Reverse transcription‑quantitative polymerase chain reaction and western blotting results showed that the expression levels of glucose‑regulated protein 78, C/EBP homologous protein and cysteinyl asparate specific proteinase‑3/‑9/‑12, were increased in MC3T3‑E1 cells following PA treatment. The treatment of the cells with PA resulted in an activation of the ERS and the JNK signaling pathway. These pathways were effectively suppressed by co‑incubation with LBP. Taken together, PA may cause ERS, in cell apoptosis, and it may further activate the JNK signaling pathway. LBP reversed PA‑induced apoptosis in MC3T3‑E1 cells through inhibition of the activation of the ERS‑mediated JNK signaling pathway.
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1 

Rachner TD, Khosla S and Hofbauer LC: Osteoporosis: Now and the future. Lancet. 377:1276–1287. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Ray NF, Chan JK, Thamer M and Melton LJ III: Medical expenditures for the treatment of osteoporotic fractures in the United States in 1995: Report from the National Osteoporosis Foundation. J Bone Miner Res. 12:24–35. 1997. View Article : Google Scholar : PubMed/NCBI

3 

Ravn P, Cizza G, Bjarnason NH, Thompson D, Daley M, Wasnich RD, McClung M, Hosking D, Yates AJ and Christiansen C: Low body mass index is an important risk factor for low bone mass and increased bone loss in early postmenopausal women. Early postmenopausal intervention cohort (EPIC) study group. J Bone Miner Res. 14:1622–1627. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Aguirre L, Napoli N, Waters D, Qualls C, Villareal DT and Armamento-Villareal R: Increasing adiposity is associated with higher adipokine levels and lower bone mineral density in obese older adults. J Clin Endocrinol Metab. 99:3290–3297. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Cohen A, Dempster DW, Recker RR, Lappe JM, Zhou H, Zwahlen A, Müller R, Zhao B, Guo X, Lang T, et al: Abdominal fat is associated with lower bone formation and inferior bone quality in healthy premenopausal women: A transiliac bone biopsy study. J Clin Endocrinol Metab. 98:2562–2572. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Arner P and Rydén M: Fatty Acids, Obesity and insulin resistance. Obes Facts. 8:147–155. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Elbaz A, Wu X, Rivas D, Gimble JM and Duque G: Inhibition of fatty acid biosynthesis prevents adipocyte lipotoxicity on human osteoblasts in vitro. J Cell Mol Med. 14:982–991. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Sharma S, Tandon VR, Mahajan S, Mahajan V and Mahajan A: Obesity: Friend or foe for osteoporosis. J Midlife Health. 5:6–9. 2014.PubMed/NCBI

9 

Kikuta S, Tanaka N, Kazama T, Kazama M, Kano K, Ryu J, Tokuhashi Y and Matsumoto T: Osteogenic effects of dedifferentiated fat cell transplantation in rabbit models of bone defect and ovariectomy-induced osteoporosis. Tissue Eng Part A. 19:1792–1802. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Lubkowska A, Dobek A, Mieszkowski J, Garczynski W and Chlubek D: Adiponectin as a biomarker of osteoporosis in postmenopausal women: Controversies. Dis Markers. 2014:9751782014. View Article : Google Scholar : PubMed/NCBI

11 

Kim JE, Ahn MW, Baek SH, Lee IK, Kim YW, Kim JY, Dan JM and Park SY: AMPK activator, AICAR, inhibits palmitate-induced apoptosis in osteoblast. Bone. 43:394–404. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Martino L, Masini M, Novelli M, Beffy P, Bugliani M, Marselli L, Masiello P, Marchetti P and De Tata V: Palmitate activates autophagy in INS-1E β-cells and in isolated rat and human pancreatic islets. PLoS One. 7:e361882012. View Article : Google Scholar : PubMed/NCBI

13 

Simon-Szabó L, Kokas M, Mandl J, Kéri G and Csala M: Metformin attenuates palmitate-induced endoplasmic reticulum stress, serine phosphorylation of IRS-1 and apoptosis in rat insulinoma cells. PLoS One. 9:e978682014. View Article : Google Scholar : PubMed/NCBI

14 

Zhang H, Li H, Liu X and Bi J: Effect of caspase-9 inhibition on endoplasmic reticulum stress induced cortical neuronal injury in rats. Int J Clin Exp Med. 6:546–551. 2013.PubMed/NCBI

15 

Wu H, Tang Q, Yang J, Ding J, Ye M and Dong W: Atorvastatin ameliorates myocardial ischemia/reperfusion injury through attenuation of endoplasmic reticulum stress-induced apoptosis. Int J Clin Exp Med. 7:4915–4923. 2014.PubMed/NCBI

16 

Wu CX, Wang TF and Yu JQ: Lycium barbarum polysaccharide pretreatment attenuates cerebral ischemic reperfusion injury by inhibiting apoptosis in mice. Zhong Yao Cai. 38:1454–1459. 2015.(In Chinese). PubMed/NCBI

17 

Zhao R, Cai Y, Shao X and Ma B: Improving the activity of Lycium barbarum polysaccharide on sub-health mice. Food Funct. 6:2033–2040. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Bie M, Lv Y, Ren C, Xing F, Cui Q, Xiao J and So KF: Lycium barbarum polysaccharide improves bipolar pulse current-induced microglia cell injury through modulating autophagy. Cell Transplant. 24:419–428. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Li XM, Ma YL and Liu XJ: Effect of the Lycium barbarum polysaccharides on age-related oxidative stress in aged mice. J Ethnopharmacol. 111:504–511. 2007. View Article : Google Scholar : PubMed/NCBI

20 

Mazziotti G, Bilezikian J, Canalis E, Cocchi D and Giustina A: New understanding and treatments for osteoporosis. Endocrine. 41:58–69. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Halade GV, Rahman MM, Williams PJ and Fernandes G: High fat diet-induced animal model of age-associated obesity and osteoporosis. J Nutr Biochem. 21:1162–1169. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Patsch JM, Kiefer FW, Varga P, Pail P, Rauner M, Stupphann D, Resch H, Moser D, Zysset PK, Stulnig TM and Pietschmann P: Increased bone resorption and impaired bone microarchitecture in short-term and extended high-fat diet-induced obesity. Metabolism. 60:243–249. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Boot-Handford RP and Briggs MD: The unfolded protein response and its relevance to connective tissue diseases. Cell Tissue Res. 339:197–211. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Thuaud F, Bernard Y, Türkeri G, Dirr R, Aubert G, Cresteil T, Baguet A, Tomasetto C, Svitkin Y, Sonenberg N, et al: Synthetic analogue of rocaglaol displays a potent and selective cytotoxicity in cancer cells: Involvement of apoptosis inducing factor and caspase-12. J Med Chem. 52:5176–5187. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jing L and Jia XW: Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway. Mol Med Rep 17: 2415-2421, 2018.
APA
Jing, L., & Jia, X. (2018). Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway. Molecular Medicine Reports, 17, 2415-2421. https://doi.org/10.3892/mmr.2017.8128
MLA
Jing, L., Jia, X."Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway". Molecular Medicine Reports 17.2 (2018): 2415-2421.
Chicago
Jing, L., Jia, X."Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway". Molecular Medicine Reports 17, no. 2 (2018): 2415-2421. https://doi.org/10.3892/mmr.2017.8128
Copy and paste a formatted citation
x
Spandidos Publications style
Jing L and Jia XW: Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway. Mol Med Rep 17: 2415-2421, 2018.
APA
Jing, L., & Jia, X. (2018). Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway. Molecular Medicine Reports, 17, 2415-2421. https://doi.org/10.3892/mmr.2017.8128
MLA
Jing, L., Jia, X."Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway". Molecular Medicine Reports 17.2 (2018): 2415-2421.
Chicago
Jing, L., Jia, X."Lycium barbarum polysaccharide arbitrates palmitate-induced apoptosis in MC3T3‑E1 cells through decreasing the activation of ERS‑mediated apoptosis pathway". Molecular Medicine Reports 17, no. 2 (2018): 2415-2421. https://doi.org/10.3892/mmr.2017.8128
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