Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2018 Volume 17 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2018 Volume 17 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction

  • Authors:
    • Lejing Li
    • Shoulian Zhu
    • Ying Li
    • Wenyuan Cao
    • Xiaoli Qiao
  • View Affiliations / Copyright

    Affiliations: Department of Acupuncture and Moxibustio, Linzi District People's Hospital, Linzi, Shandong 255400, P.R. China, Department of Neurology, Linzi District People's Hospital, Linzi, Shandong 255400, P.R. China, Department of Traditional Chinese Medicine, Linzi District People's Hospital, Linzi, Shandong 255400, P.R. China
  • Pages: 2586-2592
    |
    Published online on: November 22, 2017
       https://doi.org/10.3892/mmr.2017.8133
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Antioxidant of bamboo leaves (AOB) has been proven to have antioxidant activity and an inhibitory effect on free radicals that induce deterioration of macromolecules. The multi‑target regulation of microRNAs (miRs) in the complicated process of vasculogenesis and angiogenesis lead to the use of miRNA therapy in vascular development. In the present study, the role of miRNAs on early embryo vascular development upon AOB stimulation was investigated. For this purpose, mouse embryonic stem cells were spontaneously differentiated as embryoid bodies (EBs) and were examined by phase contrast microscopy. miR‑146a mimic and scramble control were transfected into EBs and potential targets of miR‑146a were predicted. Cell proliferation and migration were detected by cell viability and wound‑healing and migration assays, respectively. Angiogenesis was determined by the Spheroid sprouting assay. It was demonstrated that EBs transfected with miR‑146a mimic had an increased growth rate compared with the control cells. miR‑146a‑transfected cells were very susceptible to AOB treatment. Furthermore, among the predicted miR‑146a targets, platelet‑derived growth factor receptor alpha (PDGFRA) was identified as a bona fide target of miR‑146a. In conclusion, PDGFRA was demonstrated to participate in the modulation of cell migration and proliferation of mouse EBs. The present study expanded the current understanding of AOB biology and elucidated the mechanisms underlying early embryo vascular development upon AOB stimulation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Ma X, Wang E, Lu Y, Wang Y, Ou S and Yan R: Acylation of antioxidant of bamboo leaves with fatty acids by lipase and the acylated derivatives' efficiency in the inhibition of acrylamide formation in fried potato crisps. PLoS One. 10:e01306802015. View Article : Google Scholar : PubMed/NCBI

2 

Liu L, Xia B, Jin C and Zhang Y and Zhang Y: Chemical acylation of water-soluble antioxidant of bamboo leaves (AOB-w) and functional evaluation of oil-soluble AOB (cAOB-o). J Food Sci. 79:C1886–C1894. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Zhang Y, Luo Z, Shao Z, Yu C and Wang S: Effects of antioxidants of bamboo leaves and flavonoids on 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) formation in chemical model systems. J Agric Food Chem. 62:4798–4802. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Hirota S and Ogawa M: Activin A in combination with OP9 cells facilitates development of Flk-1(+) PDGFRα(−) and Flk-1(+) PDGFRα(+) hematopoietic mesodermal cells from murine embryonic stem cells. Biochem Biophys Res Commun. 467:583–588. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Moriya J and Ferrara N: Inhibition of protein kinase C enhances angiogenesis induced by platelet-derived growth factor C in hyperglycemic endothelial cells. Cardiovasc Diabetol. 14:192015. View Article : Google Scholar : PubMed/NCBI

6 

Ro S, Park C, Young D, Sanders KM and Yan W: Tissue-dependent paired expression of miRNAs. Nucleic Acids Res. 35:5944–5953. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Mallory AC and Vaucheret H: MicroRNAs: Something important between the genes. Curr Opin Plant Biol. 7:120–125. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Hayes J, Peruzzi PP and Lawler S: MicroRNAs in cancer: Biomarkers, functions and therapy. Trends Mol Med. 20:460–469. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Chang TY, Huang TS, Wang HW, Chang SJ, Lo HH, Chiu YL, Wang YL, Hsiao CD, Tsai CH, Chan CH, et al: miRNome traits analysis on endothelial lineage cells discloses biomarker potential circulating microRNAs which affect progenitor activities. BMC Genomics. 15:8022014. View Article : Google Scholar : PubMed/NCBI

10 

Sundaravinayagam D, Kim HR, Wu T, Kim HH, Lee HS, Jun S, Cha JH, Kee Y, You HJ and Lee JH: miR146a-mediated targeting of FANCM during inflammation compromises genome integrity. Oncotarget. 7:45976–45994. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Zhang B, Kirov S and Snoddy J: WebGestalt: An integrated system for exploring gene sets in various biological contexts. Nucleic Acids Res. 33(Web Server Issue): W741–W748. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Scmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

McCarthy N, Liu JS, Richarte AM, Eskiocak B, Lovely CB, Tallquist MD and Eberhart JK: Pdgfra and Pdgfrb genetically interact during craniofacial development. Dev Dyn. 245:641–652. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Dziedzicka D, Markouli C, Barbé L, Spits C, Sermon K and Geens M: A high proliferation rate is critical for reproducible and standardized embryoid body formation from laminin-521-based human pluripotent stem cell cultures. Stem Cell Rev. 12:721–730. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Boxman J, Sagy N, Achanta S, Vadigepalli R and Nachman I: Integrated live imaging and molecular profiling of embryoid bodies reveals a synchronized progression of early differentiation. Sci Rep. 6:316232016. View Article : Google Scholar : PubMed/NCBI

16 

Moon SH, Ju J, Park SJ, Bae D, Chung HM and Lee SH: Optimizing human embryonic stem cells differentiation efficiency by screening size-tunable homogenous embryoid bodies. Biomaterials. 35:5987–5997. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Kim JE, Lee JM and Chung BG: Microwell arrays for uniform-sized embryoid body-mediated endothelial cell differentiation. Biomed Microdevices. 16:559–566. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Crabbé MA, Gijbels K, Visser A, Craeye D, Walbers S, Pinxteren J, Deans RJ, Annaert W and Vaes BL: Using miRNA-mRNA interaction analysis to link biologically relevant miRNAs to stem cell identity testing for next-generation culturing development. Stem Cells Transl Med. 5:709–722. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Yang Y, Xing Y, Liang C, Hu L, Xu F and Chen Y: Crucial microRNAs and genes of human primary breast cancer explored by microRNA-mRNA integrated analysis. Tumour Biol. 36:5571–5579. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Tutar L, Tutar E, Özgür A and Tutar Y: Therapeutic targeting of microRNAs in cancer: Future perspectives. Drug Dev Res. 76:382–388. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Breda J, Rzepiela AJ, Gumienny R, van Nimwegen E and Zavolan M: Quantifying the strength of miRNA-target interactions. Methods. 85:90–99. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Miao CG, Xiong YY, Yu H, Zhang XL, Qin MS, Song TW and Du CL: Critical roles of microRNAs in the pathogenesis of systemic sclerosis: New advances, challenges and potential directions. Int Immunopharmacol. 28:626–633. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Rudat C, Norden J, Taketo MM and Kispert A: Epicardial function of canonical Wnt-, Hedgehog-, Fgfr1/2- and Pdgfra-signalling. Cardiovasc Res. 100:411–421. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Li F, He Z, Li Y, Liu P, Chen F, Wang M, Zhu H, Ding X, Wangensteen KJ, Hu Y and Wang X: Combined activin A/LiCl/Noggin treatment improves production of mouse embryonic stem cell-derived definitive endoderm cells. J Cell Biochem. 112:1022–1034. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Zhu K, Pan Q, Zhang X, Kong LQ, Fan J, Dai Z, Wang L, Yang XR, Hu J, Wan JL, et al: MiR-146a enhances angiogenic activity of endothelial cells in hepatocellular carcinoma by promoting PDGFRA expression. Carcinogenesis. 34:2071–2079. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Li L, Zhu S, Li Y, Cao W and Qiao X: Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction. Mol Med Rep 17: 2586-2592, 2018.
APA
Li, L., Zhu, S., Li, Y., Cao, W., & Qiao, X. (2018). Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction. Molecular Medicine Reports, 17, 2586-2592. https://doi.org/10.3892/mmr.2017.8133
MLA
Li, L., Zhu, S., Li, Y., Cao, W., Qiao, X."Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction". Molecular Medicine Reports 17.2 (2018): 2586-2592.
Chicago
Li, L., Zhu, S., Li, Y., Cao, W., Qiao, X."Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction". Molecular Medicine Reports 17, no. 2 (2018): 2586-2592. https://doi.org/10.3892/mmr.2017.8133
Copy and paste a formatted citation
x
Spandidos Publications style
Li L, Zhu S, Li Y, Cao W and Qiao X: Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction. Mol Med Rep 17: 2586-2592, 2018.
APA
Li, L., Zhu, S., Li, Y., Cao, W., & Qiao, X. (2018). Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction. Molecular Medicine Reports, 17, 2586-2592. https://doi.org/10.3892/mmr.2017.8133
MLA
Li, L., Zhu, S., Li, Y., Cao, W., Qiao, X."Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction". Molecular Medicine Reports 17.2 (2018): 2586-2592.
Chicago
Li, L., Zhu, S., Li, Y., Cao, W., Qiao, X."Downregulation of miR‑146a promotes proliferation and migration of AOB‑treated embryoid body via PDGFRA induction". Molecular Medicine Reports 17, no. 2 (2018): 2586-2592. https://doi.org/10.3892/mmr.2017.8133
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team