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Article

IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts

  • Authors:
    • Cheng Yao
    • Sangbum Han
    • Chi‑Hyun Park
    • Ye‑Ji Kim
    • Dong Hun Lee
    • Jin Ho Chung
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Seoul National University College of Medicine, Seoul 110‑744, Republic of Korea
  • Pages: 2581-2585
    |
    Published online on: November 22, 2017
       https://doi.org/10.3892/mmr.2017.8136
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Abstract

Pattern recognition receptors (PRRs) are part of the immune system. They can recognize pathogen‑associated molecular patterns (PAMPs). Toll‑like receptors (TLRs) and retinoic acid‑inducible gene 1 (RIG‑1)‑like receptors (RLRs) are 2 types of PRR in the innate immune system. Double‑stranded RNA (dsRNA) can exist as a PAMP, including dsRNA viruses. dsRNA is known as a ligand not only for TLR3 but also for RLRs, including melanoma differentiation‑associated gene 5 and RIG‑1. Collagen is the main structural protein in the extracellular space in the skin. Recently, it was reported that treatment of a synthetic dsRNA, poly(I:C), decreases procollagen expression in skin fibroblasts. However, signaling pathways involved in this process have not yet been fully elucidated. The present study further explored the underlying signaling pathways involved in the processes. It was demonstrated by western blotting that treatment of poly(I:C), but not another PAMP, Pam3CSK4, inhibited procollagen expression in cultured human skin fibroblasts. Treatment of poly(I:C)and Pam3CSK4 induced activation of the mitogen‑activated protein kinases and the nuclear factor‑κB pathways. However, only poly(I:C), but not Pam3CSK4, induced the activation of the interferon regulatory factor 3 (IRF3) pathway. By using specific inhibitors, it was demonstrated that inhibition of IRF3 pathway relieved poly(I:C)‑induced procollagen reduction. In conclusion, IRF3 signaling pathway serves an important role in poly(I:C)‑induced procollagen reduction in skin fibroblasts. This suggests that the IRF3 signaling pathway may be a key target for collagen regulation in the skin.
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Copy and paste a formatted citation
Spandidos Publications style
Yao C, Han S, Park CH, Kim YJ, Lee DH and Chung JH: IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts. Mol Med Rep 17: 2581-2585, 2018.
APA
Yao, C., Han, S., Park, C., Kim, Y., Lee, D.H., & Chung, J.H. (2018). IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts. Molecular Medicine Reports, 17, 2581-2585. https://doi.org/10.3892/mmr.2017.8136
MLA
Yao, C., Han, S., Park, C., Kim, Y., Lee, D. H., Chung, J. H."IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts". Molecular Medicine Reports 17.2 (2018): 2581-2585.
Chicago
Yao, C., Han, S., Park, C., Kim, Y., Lee, D. H., Chung, J. H."IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts". Molecular Medicine Reports 17, no. 2 (2018): 2581-2585. https://doi.org/10.3892/mmr.2017.8136
Copy and paste a formatted citation
x
Spandidos Publications style
Yao C, Han S, Park CH, Kim YJ, Lee DH and Chung JH: IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts. Mol Med Rep 17: 2581-2585, 2018.
APA
Yao, C., Han, S., Park, C., Kim, Y., Lee, D.H., & Chung, J.H. (2018). IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts. Molecular Medicine Reports, 17, 2581-2585. https://doi.org/10.3892/mmr.2017.8136
MLA
Yao, C., Han, S., Park, C., Kim, Y., Lee, D. H., Chung, J. H."IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts". Molecular Medicine Reports 17.2 (2018): 2581-2585.
Chicago
Yao, C., Han, S., Park, C., Kim, Y., Lee, D. H., Chung, J. H."IRF3 signaling pathway serves an important role in poly(I:C)-induced procollagen reduction in human skin fibroblasts". Molecular Medicine Reports 17, no. 2 (2018): 2581-2585. https://doi.org/10.3892/mmr.2017.8136
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