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Article

MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway

  • Authors:
    • Fengli Che
    • Huishan Du
    • Weidong Zhang
    • Zhe Cheng
    • Yanna Tong
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Beijing Luhe Hospital, Capital Medical University, Beijing 101145, P.R. China
  • Pages: 2724-2730
    |
    Published online on: November 22, 2017
       https://doi.org/10.3892/mmr.2017.8138
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Abstract

In the present study, the expression of microRNA (miR)‑132 and the mechanism by which it modifies angiogenesis in patients with ischemic cerebrovascular disease (ICD) was investigated. RNA isolation and reverse transcription‑quantitative polymerase chain reaction were used to measure miR‑132 expression in patients with ICD. Inflammatory factors were measured using ELISA kits and western blotting measured B‑cell lymphoma‑2 (Bcl‑2)‑associated X/Bcl‑2 ratio (Bax/Bcl‑2 ratio), nuclear factor (NF)‑κB p65, matrix metalloproteinase‑9 (MMP‑9), vascular cell adhesion molecule‑1 (VCAM‑1) and protein expression of inducible nitric oxide synthase (iNOS), and vascular endothelial growth factor (VEGF) protein expression. miR‑132 expression in patients with ICD was lower compared with healthy volunteers. PC12 cells were used to create an oxygen glucose deprivation (OGD) model. miR‑132 overexpression in an in vitro model was able to reduce tumor necrosis factor‑a, interleukin (IL)‑1β, IL‑6, IL‑8, cyclooxygenase‑2, caspase‑3 and caspase‑9 levels, suppress Bax/Bcl‑2 ratio, NF‑κB p65, MMP‑9, VCAM‑1, iNOS, VEGF protein expression. The results suggested that miR‑132 may modify angiogenesis in patients with ICD by suppressing the NF‑κB pathway and promoting the VEGF pathway, and may develop into a therapy for ICD in future research.
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Copy and paste a formatted citation
Spandidos Publications style
Che F, Du H, Zhang W, Cheng Z and Tong Y: MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway. Mol Med Rep 17: 2724-2730, 2018.
APA
Che, F., Du, H., Zhang, W., Cheng, Z., & Tong, Y. (2018). MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway. Molecular Medicine Reports, 17, 2724-2730. https://doi.org/10.3892/mmr.2017.8138
MLA
Che, F., Du, H., Zhang, W., Cheng, Z., Tong, Y."MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway". Molecular Medicine Reports 17.2 (2018): 2724-2730.
Chicago
Che, F., Du, H., Zhang, W., Cheng, Z., Tong, Y."MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway". Molecular Medicine Reports 17, no. 2 (2018): 2724-2730. https://doi.org/10.3892/mmr.2017.8138
Copy and paste a formatted citation
x
Spandidos Publications style
Che F, Du H, Zhang W, Cheng Z and Tong Y: MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway. Mol Med Rep 17: 2724-2730, 2018.
APA
Che, F., Du, H., Zhang, W., Cheng, Z., & Tong, Y. (2018). MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway. Molecular Medicine Reports, 17, 2724-2730. https://doi.org/10.3892/mmr.2017.8138
MLA
Che, F., Du, H., Zhang, W., Cheng, Z., Tong, Y."MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway". Molecular Medicine Reports 17.2 (2018): 2724-2730.
Chicago
Che, F., Du, H., Zhang, W., Cheng, Z., Tong, Y."MicroRNA-132 modifies angiogenesis in patients with ischemic cerebrovascular disease by suppressing the NF‑κB and VEGF pathway". Molecular Medicine Reports 17, no. 2 (2018): 2724-2730. https://doi.org/10.3892/mmr.2017.8138
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