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Article

Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry

  • Authors:
    • Yi‑Lei Li
    • Rui‑Qun Qi
    • Yang Yang
    • He‑Xiao Wang
    • Hang‑Hang Jiang
    • Zheng‑Xiu Li
    • Bi‑Huan Xiao
    • Song Zheng
    • Yu‑Xiao Hong
    • Jiu‑Hong Li
    • Hong‑Duo Chen
    • Xing‑Hua Gao
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 2651-2659
    |
    Published online on: November 27, 2017
       https://doi.org/10.3892/mmr.2017.8159
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Abstract

In the clinic, vitiligo is characterized by two stages: Stable and progressive. The pathogenesis of vitiligo is still not clear. Here, we identified serum markers of vitiligo by screening for differentially expressed proteins in patients with vitiligo compared to healthy individuals. Serum samples were collected from patients with vitiligo (n=10 for both the stable and progressive stages) and healthy individuals (n=10). Two‑dimensional gel electrophoresis followed by matrix‑assisted laser desorption/ionization time‑of‑flight mass spectrometry and western blotting were used to validate the differential expression of the proteins in the serum (n=20 each, at both stages for patients and healthy individuals). A total of 48 differentially expressed proteins were identified by gel image analysis. There were 28 differentially expressed proteins in patients with progressive vitiligo (PV) and 13 differentially expressed proteins in patients with stable vitiligo (SV) compared with that in healthy individuals. Additionally, 7 differentially expressed proteins were identified in patients with PV compared with those in patients with SV. The western blotting results showed that Peroxiredoxin‑6, apolipoprotein L1, apolipoprotein E and mannose‑binding protein were differentially expressed in patients with different stages of vitiligo. Our results showed that change serum levels of several proteins might be useful as biomarkers or in understanding the pathogenesis of vitiligo.
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Copy and paste a formatted citation
Spandidos Publications style
Li YL, Qi RQ, Yang Y, Wang HX, Jiang HH, Li ZX, Xiao BH, Zheng S, Hong YX, Li JH, Li JH, et al: Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry. Mol Med Rep 17: 2651-2659, 2018.
APA
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z. ... Gao, X. (2018). Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry. Molecular Medicine Reports, 17, 2651-2659. https://doi.org/10.3892/mmr.2017.8159
MLA
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z., Xiao, B., Zheng, S., Hong, Y., Li, J., Chen, H., Gao, X."Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry". Molecular Medicine Reports 17.2 (2018): 2651-2659.
Chicago
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z., Xiao, B., Zheng, S., Hong, Y., Li, J., Chen, H., Gao, X."Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry". Molecular Medicine Reports 17, no. 2 (2018): 2651-2659. https://doi.org/10.3892/mmr.2017.8159
Copy and paste a formatted citation
x
Spandidos Publications style
Li YL, Qi RQ, Yang Y, Wang HX, Jiang HH, Li ZX, Xiao BH, Zheng S, Hong YX, Li JH, Li JH, et al: Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry. Mol Med Rep 17: 2651-2659, 2018.
APA
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z. ... Gao, X. (2018). Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry. Molecular Medicine Reports, 17, 2651-2659. https://doi.org/10.3892/mmr.2017.8159
MLA
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z., Xiao, B., Zheng, S., Hong, Y., Li, J., Chen, H., Gao, X."Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry". Molecular Medicine Reports 17.2 (2018): 2651-2659.
Chicago
Li, Y., Qi, R., Yang, Y., Wang, H., Jiang, H., Li, Z., Xiao, B., Zheng, S., Hong, Y., Li, J., Chen, H., Gao, X."Screening and identification of differentially expressed serum proteins in patients with vitiligo using two‑dimensional gel electrophoresis coupled with mass spectrometry". Molecular Medicine Reports 17, no. 2 (2018): 2651-2659. https://doi.org/10.3892/mmr.2017.8159
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