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Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model

  • Authors:
    • Lingdan Dong
    • Xianglin Cheng
    • Long Zhou
    • Yanhong Hu
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, First People's Hospital of Jingzhou, First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China , Department of Neurology, First People's Hospital of Jingzhou, First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China, Department of Pathology, First People's Hospital of Jingzhou, First Affiliated Hospital of Yangtze University, Jingzhou, Hubei 434000, P.R. China, Nursing Department of Medical School of Yangtze University, Jingzhou, Hubei 434000, P.R. China
    Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2465-2471
    |
    Published online on: November 27, 2017
       https://doi.org/10.3892/mmr.2017.8162
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Abstract

Erythropoietin-producing hepatocyte receptor B (EphB)/ephrinB reverse signaling has been revealed to be activated in chronic ocular hypertension (COH) by increasing the apoptosis of retinal ganglion cells (RGCs). However, the exact mechanism is not well understood. The present study investigated the involvement of Ca2+ channels in the apoptosis of RGCs induced by EphB/ephrinB reverse signaling in a rat CHO model, which was established by cauterizing 3 out of the 4 episcleral veins. The expression levels of four voltage‑gated Ca2+ channel subunits (Cav3.1‑3.3 and Cav1.2) were detected using immunofluorescence and western blot analysis. TUNEL staining was performed to assess RGC apoptosis following an injection with the T type Ca2+ channel blocker. Ca2+ channels, mainly the T type, were upregulated in COH rat retinas when compared with the sham group (P<0.01). Additionally, the Cav3.2 subunit of T type calcium channels was predominantly expressed in Müller cells and RGCs, such as ephrinB2. Furthermore, an intravitreal injection of the Ca2+ channel blocker Mibefradil (3 µM) reduced EphB2‑fragment crystallizable region‑induced RGC apoptosis in normal rats. Thus, the results suggest that Ca2+ channels in a COH model may be a pathway involved in ephrinB/EphB signaling‑induced RGC apoptosis.
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Copy and paste a formatted citation
Spandidos Publications style
Dong L, Cheng X, Zhou L and Hu Y: Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model. Mol Med Rep 17: 2465-2471, 2018.
APA
Dong, L., Cheng, X., Zhou, L., & Hu, Y. (2018). Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model. Molecular Medicine Reports, 17, 2465-2471. https://doi.org/10.3892/mmr.2017.8162
MLA
Dong, L., Cheng, X., Zhou, L., Hu, Y."Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model". Molecular Medicine Reports 17.2 (2018): 2465-2471.
Chicago
Dong, L., Cheng, X., Zhou, L., Hu, Y."Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model". Molecular Medicine Reports 17, no. 2 (2018): 2465-2471. https://doi.org/10.3892/mmr.2017.8162
Copy and paste a formatted citation
x
Spandidos Publications style
Dong L, Cheng X, Zhou L and Hu Y: Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model. Mol Med Rep 17: 2465-2471, 2018.
APA
Dong, L., Cheng, X., Zhou, L., & Hu, Y. (2018). Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model. Molecular Medicine Reports, 17, 2465-2471. https://doi.org/10.3892/mmr.2017.8162
MLA
Dong, L., Cheng, X., Zhou, L., Hu, Y."Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model". Molecular Medicine Reports 17.2 (2018): 2465-2471.
Chicago
Dong, L., Cheng, X., Zhou, L., Hu, Y."Calcium channels are involved in EphB/ephrinB reverse signaling‑induced apoptosis in a rat chronic ocular hypertension model". Molecular Medicine Reports 17, no. 2 (2018): 2465-2471. https://doi.org/10.3892/mmr.2017.8162
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