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Article

Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts

  • Authors:
    • Yan Zhou
    • Huiyu Zhang
    • Gang Zhang
    • Yong He
    • Ping Zhang
    • Ziqi Sun
    • Yuxia Gao
    • Yinghui Tan
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Stomatology, General Hospital of Chengdu Military Region of PLA, Chengdu, Sichuan 610083, P.R. China, Jiebao Biotechnology, Wuhan, Hubei 430012, P.R. China
  • Pages: 3246-3254
    |
    Published online on: December 6, 2017
       https://doi.org/10.3892/mmr.2017.8205
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Abstract

Periodontal diseases comprise mixed bacterial infections mainly caused by Gram‑negative anaerobic bacteria. Lipopolysaccharides (LPS) are important virulence factors and periodontal pathogens, which change local cytokine levels and promote osteoblast apoptosis, thereby leading to an imbalance in bone remodeling mechanisms and accelerating bone loss. Calcitonin gene‑related peptide (CGRP) is a vasoactive neuropeptide that is released from sensory nerves and has a positive effect on osteoblast proliferation and differentiation. In addition, this small molecule peptide is an important immune regulator in the inflammatory response. The aim of the present study was to assess the in vitro effects of CGRP on Porphyromonas gingivalis (Pg)LPS‑induced osteoblast apoptosis. Osteoblast cultures were stimulated either with various concentrations of PgLPS (0, 25, 50, 100, 500 and 1,000 ng/ml) for 48 h or with 500 ng/ml PgLPS for various lengths of time (0, 6, 12, 24, 48 and 72 h). The PgLPS‑stimulated cells were pretreated with different concentrations of CGRP (0, 1, 10, 100 and 1,000 nM) and cell viability and apoptotic rates were measured by Cell Counting kit‑8 assays and flow cytometry, respectively. CGRP, cleaved (c)‑Caspase‑8 and c‑Caspase‑3 protein expression levels were analyzed by western blotting. Changes in cytokine expression levels, which included tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6, monocyte chemotactic protein (MCP)‑1 and MCP‑2, were measured by ELISA. PgLPS was demonstrated to inhibit osteoblast viability and promote apoptosis in a time‑ and concentration‑dependent manner. CGRP expression was revealed to reduce PgLPS‑induced cytostatic activity and apoptosis in osteoblasts. CGRP also suppressed the PgLPS‑induced release of TNF‑α and inhibited the activation of c‑Caspase‑3 and c‑Caspase‑8, thus preventing apoptosis in osteoblasts. CGRP may be an important neuropeptide in bone remodeling and may reduce osteoblast apoptosis in inflammatory conditions. These results may provide a solid foundation for CGRP to serve as a new target for the treatment of periodontitis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Y, Zhang H, Zhang G, He Y, Zhang P, Sun Z, Gao Y and Tan Y: Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts. Mol Med Rep 17: 3246-3254, 2018.
APA
Zhou, Y., Zhang, H., Zhang, G., He, Y., Zhang, P., Sun, Z. ... Tan, Y. (2018). Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts. Molecular Medicine Reports, 17, 3246-3254. https://doi.org/10.3892/mmr.2017.8205
MLA
Zhou, Y., Zhang, H., Zhang, G., He, Y., Zhang, P., Sun, Z., Gao, Y., Tan, Y."Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts". Molecular Medicine Reports 17.2 (2018): 3246-3254.
Chicago
Zhou, Y., Zhang, H., Zhang, G., He, Y., Zhang, P., Sun, Z., Gao, Y., Tan, Y."Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts". Molecular Medicine Reports 17, no. 2 (2018): 3246-3254. https://doi.org/10.3892/mmr.2017.8205
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Y, Zhang H, Zhang G, He Y, Zhang P, Sun Z, Gao Y and Tan Y: Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts. Mol Med Rep 17: 3246-3254, 2018.
APA
Zhou, Y., Zhang, H., Zhang, G., He, Y., Zhang, P., Sun, Z. ... Tan, Y. (2018). Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts. Molecular Medicine Reports, 17, 3246-3254. https://doi.org/10.3892/mmr.2017.8205
MLA
Zhou, Y., Zhang, H., Zhang, G., He, Y., Zhang, P., Sun, Z., Gao, Y., Tan, Y."Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts". Molecular Medicine Reports 17.2 (2018): 3246-3254.
Chicago
Zhou, Y., Zhang, H., Zhang, G., He, Y., Zhang, P., Sun, Z., Gao, Y., Tan, Y."Calcitonin gene‑related peptide reduces Porphyromonas gingivalis LPS‑induced TNF‑α release and apoptosis in osteoblasts". Molecular Medicine Reports 17, no. 2 (2018): 3246-3254. https://doi.org/10.3892/mmr.2017.8205
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