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Article Open Access

Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer

  • Authors:
    • Xiao Qiu
    • Jue‑Rong Feng
    • Fan Wang
    • Peng‑Fei Chen
    • Xiao‑Xing Chen
    • Rui Zhou
    • Ying Chang
    • Jing Liu
    • Qiu Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Zhongnan Hospital of Wuhan University, The Hubei Clinical Center and Key Laboratory of Intestinal and Colorectal Diseases, Wuhan, Hubei 430071, P.R. China
    Copyright: © Qiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3028-3034
    |
    Published online on: December 7, 2017
       https://doi.org/10.3892/mmr.2017.8210
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Abstract

The present study aimed to identify differentially expressed genes (DEGs) in colorectal cancer (CRC) and provide novel prognostic biomarkers for CRC. The microarray dataset GSE41258 was used to screen DEGs of CRC. Subsequently, a protein‑protein interaction network of DEGs and Gene Ontology analysis were performed to identify hub genes and associated biological processes. Nebulette (NEBL) and complement C1q like 1 (C1QL1) were validated using reverse transcription‑quantitative polymerase chain reaction in patients with CRC. Survival analysis was performed for the two hub genes. GSE41258 dataset included 182 CRC samples and 54 normal tissues. A total of 759 DEGs, including 279 upregulated and 480 downregulated were screened between both groups. NEBL and C1QL1 were identified as the two hub genes and upregulated genes involved in various biological processes, including ‘regulation of biological quality’ and ‘response to stimulus’, respectively. Additionally, the overexpression of NEBL and C1QL1 in experimental validation was consistent with the aforementioned bioinformatics analysis results. Survival analysis suggested that overexpressed NEBL in patients with CRC was associated with a positive prognosis for overall survival. In conclusion, CRC was associated with a large group of DEGs. From the upregulated genes, overexpressed NEBL in patients CRC indicated a positive prognosis for overall survival and may be used as a prognostic biomarker for patients with CRC.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, Nakamura Y, White R, Smits AM and Bos JL: Genetic alterations during colorectal-tumor development. N Engl J Med. 319:525–532. 1988. View Article : Google Scholar : PubMed/NCBI

3 

Rahmani F, Avan A, Hashemy SI and Hassanian SM: Role of Wnt/β-catenin signaling regulatory microRNAs in the pathogenesis of colorectal cancer. J Cell Physiol. 233:811–817. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Louis P, Hold GL and Flint HJ: The gut microbiota, bacterial metabolites and colorectal cancer. Nat Rev Microbiol. 12:661–672. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Grady WM and Carethers JM: Genomic and epigenetic instability in colorectal cancer pathogenesis. Gastroenterology. 135:1079–1099. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Nakayama T, Hirakawa H, Shibata K, Nazneen A, Abe K, Nagayasu T and Taguchi T: Expression of angiopoietin-like 4 (ANGPTL4) in human colorectal cancer: ANGPTL4 promotes venous invasion and distant metastasis. Oncol Rep. 25:929–935. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Gellad ZF and Provenzale D: Colorectal cancer: National and international perspective on the burden of disease and public health impact. Gastroenterology. 138:2177–2190. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Ahmed FE: miRNA as markers for the diagnostic screening of colon cancer. Expert Rev Anticancer Ther. 14:463–485. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Rawson JB and Bapat B: Epigenetic biomarkers in colorectal cancer diagnostics. Expert Rev Mol Diagn. 12:499–509. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Walther A, Johnstone E, Swanton C, Midgley R, Tomlinson I and Kerr D: Genetic prognostic and predictive markers in colorectal cancer. Nat Rev Cancer. 9:489–499. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Verdaguer H, Saurí T and Macarulla T: Predictive and prognostic biomarkers in personalized gastrointestinal cancer treatment. J Gastrointest Oncol. 8:405–417. 2017. View Article : Google Scholar : PubMed/NCBI

12 

van der Stok EP, Spaander MCW, Grünhagen DJ, Verhoef C and Kuipers EJ: Surveillance after curative treatment for colorectal cancer. Nat Rev Clin Oncol. 14:297–315. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Malapell U, Mayo de-Las-Casas C, Rocco D, Garzon M, Pisapia P, Jordana-Ariza N, Russo M, Sgariglia R, De Luca C, Pepe F, et al: Development of a gene panel for next-generation sequencing of clinically relevant mutations in cell-free DNA from cancer patients. Br J Cancer. 116:802–810. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Broderick P, Carvajal-Carmona L, Pittman AM, Webb E, Howarth K, Rowan A, Lubbe S, Spain S, Sullivan K, Fielding S, et al: A genome-wide association study shows that common alleles of SMAD7 influence colorectal cancer risk. Nat Genet. 39:1315–1317. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Snezhkina AV, Krasnov GS, Zaretsky AR, Zhavoronkov A, Nyushko KM, Moskalev AA, Karpova IY, Afremova AI, Lipatova AV, Kochetkov DV, et al: Differential expression of alternatively spliced transcripts related to energy metabolism in colorectal cancer. BMC Genomics. 17 Suppl 14:10112016. View Article : Google Scholar : PubMed/NCBI

16 

Sheffer M, Bacolod MD, Zuk O, Giardina SF, Pincas H, Barany F, Paty PB, Gerald WL, Notterman DA and Domany E: Association of survival and disease progression with chromosomal instability: A genomic exploration of colorectal cancer. Proc Natl Acad Sci USA. 106:pp. 7131–7136. 2009; View Article : Google Scholar : PubMed/NCBI

17 

Ritchie ME, Phipson B, Wu D, Hu Y, Law CW, Shi W and Smyth GK: Limma powers differential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43:e472015. View Article : Google Scholar : PubMed/NCBI

18 

Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Hari DM, Leung AM, Lee JH, Sim MS, Vuong B, Chiu CG and Bilchik AJ: AJCC cancer staging manual 7th edition criteria for colon cancer: Do the complex modifications improve prognostic assessment? J Am Coll Surg. 217:181–190. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(sT)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Goswami CP and Nakshatri H: PROGgeneV2: Enhancements on the existing database. BMC Cancer. 14:9702014. View Article : Google Scholar : PubMed/NCBI

22 

Keane MG and Johnson GJ: Early diagnosis improves survival in colorectal cancer. Practitioner. 256:15–18, 2. 2012.PubMed/NCBI

23 

Lee EJ, De Winter JM, Buck D, Jasper JR, Malik FI, Labeit S, Ottenheijm CA and Granzier H: Fast skeletal muscle troponin activation increases force of mouse fast skeletal muscle and ameliorates weakness due to nebulin-deficiency. PLoS One. 8:e558612013. View Article : Google Scholar : PubMed/NCBI

24 

Cóser VM, Meyer C, Basegio R, Menezes J, Marschalek R and Pombo-de-Oliveira MS: Nebulette is the second member of the nebulin family fused to the MLL gene in infant leukemia. Cancer Genet Cytogenet. 198:151–154. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Mastrototaro G, Liang X, Li X, Carullo P, Piroddi N, Tesi C, Gu Y, Dalton ND, Peterson KL, Poggesi C, et al: Nebulette knockout mice have normal cardiac function, but show Z-line widening and up-regulation of cardiac stress markers. Cardiovasc Res. 107:216–225. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Perrot A, Tomasov P, Villard E, Faludi R, Melacini P, Lossie J, Lohmann N, Richard P, De Bortoli M, Angelini A, et al: Mutations in NEBL encoding the cardiac Z-disk protein nebulette are associated with various cardiomyopathies. Arch Med Sci. 12:263–278. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Miao J, Fan Q, Cui Q, Zhang H, Chen L, Wang S, Guan N, Guan Y and Ding J: Newly identified cytoskeletal components are associated with dynamic changes of podocyte foot processes. Nephrol Dial Transplant. 24:3297–3305. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Aino M, Nishida E, Fujieda Y, Orimoto A, Mitani A, Noguchi T, Makino H, Murakami S, Umezawa A, Yoneda T and Saito M: Isolation and characterization of the human immature osteoblast culture system from the alveolar bones of aged donors for bone regeneration therapy. Expert Opin Biol Ther. 14:1731–1744. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Bérubé NG, Swanson XH, Bertram MJ, Kittle JD, Didenko V, Baskin DS, Smith JR and Pereira-Smith OM: Cloning and characterization of CRF, a novel C1q-related factor, expressed in areas of the brain involved in motor function. Brain Res Mol Brain Res. 63:233–240. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Yuzaki M: The C1q complement family of synaptic organizers: Not just complementary. Curr Opin Neurobiol. 45:9–15. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Sigoillot SM, Iyer K, Binda F, González-Calvo I, Talleur M, Vodjdani G, Isope P and Selimi F: The secreted protein C1QL1 and its receptor bai3 control the synaptic connectivity of excitatory inputs converging on cerebellar Purkinje cells. Cell Rep. pii:S2211–1247, 00059-100065. 2015.

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Copy and paste a formatted citation
Spandidos Publications style
Qiu X, Feng JR, Wang F, Chen PF, Chen XX, Zhou R, Chang Y, Liu J and Zhao Q: Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer. Mol Med Rep 17: 3028-3034, 2018.
APA
Qiu, X., Feng, J., Wang, F., Chen, P., Chen, X., Zhou, R. ... Zhao, Q. (2018). Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer. Molecular Medicine Reports, 17, 3028-3034. https://doi.org/10.3892/mmr.2017.8210
MLA
Qiu, X., Feng, J., Wang, F., Chen, P., Chen, X., Zhou, R., Chang, Y., Liu, J., Zhao, Q."Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer". Molecular Medicine Reports 17.2 (2018): 3028-3034.
Chicago
Qiu, X., Feng, J., Wang, F., Chen, P., Chen, X., Zhou, R., Chang, Y., Liu, J., Zhao, Q."Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer". Molecular Medicine Reports 17, no. 2 (2018): 3028-3034. https://doi.org/10.3892/mmr.2017.8210
Copy and paste a formatted citation
x
Spandidos Publications style
Qiu X, Feng JR, Wang F, Chen PF, Chen XX, Zhou R, Chang Y, Liu J and Zhao Q: Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer. Mol Med Rep 17: 3028-3034, 2018.
APA
Qiu, X., Feng, J., Wang, F., Chen, P., Chen, X., Zhou, R. ... Zhao, Q. (2018). Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer. Molecular Medicine Reports, 17, 3028-3034. https://doi.org/10.3892/mmr.2017.8210
MLA
Qiu, X., Feng, J., Wang, F., Chen, P., Chen, X., Zhou, R., Chang, Y., Liu, J., Zhao, Q."Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer". Molecular Medicine Reports 17.2 (2018): 3028-3034.
Chicago
Qiu, X., Feng, J., Wang, F., Chen, P., Chen, X., Zhou, R., Chang, Y., Liu, J., Zhao, Q."Profiles of differentially expressed genes and overexpression of NEBL indicates a positive prognosis in patients with colorectal cancer". Molecular Medicine Reports 17, no. 2 (2018): 3028-3034. https://doi.org/10.3892/mmr.2017.8210
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