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Article

Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model

  • Authors:
    • Long Sun
    • Peng Xu
    • Tinggang Fu
    • Xin Huang
    • Jie Song
    • Meng Chen
    • Xinghan Tian
    • Hongli Yin
    • Jichun Han
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Lin Yi Central Hospital, Linyi, Shandong 276400, P.R. China, Department of Medicine, Yantai Yuhuangding Hospital of Laishan Branch, Yantai, Shandong 264003, P.R. China, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China
  • Pages: 3274-3280
    |
    Published online on: December 7, 2017
       https://doi.org/10.3892/mmr.2017.8212
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Abstract

The purpose of the present study was to examine the effects of myricetin on reducing cerebral ischemia injury in a rat model. A rat model of permanent middle cerebral artery occlusion (pMCAO) was used in the present study. Rats were randomized into the following five groups: Sham, model, low‑myricetin (1 mg/kg), medium‑myricetin (5 mg/kg) and high‑myricetin (25 mg/kg) groups. Neurological deficit scores were evaluated by an examiner blinded to the experimental groups. Brain infarct size was estimated macroscopically using 2,3,5‑triphenyltetrazolium chloride staining. The levels of inflammatory factors tumor necrosis factor (TNF)‑α, interleukin (IL)‑6 and IL‑1β, and oxidative stress index superoxide dismutase (SOD), malondiadehyde (MDA), and the glutathione/glutathione disulfide (GSH/GSSG) ratio were measured by ELISA. The degree of brain cell apoptosis was determined using a terminal deoxynucleotidyl transferase dUTP nick‑end labeling assay. Protein expression levels of total or phosphorylated p38 mitogen activated protein kinase (MAPK), nuclear factor (NF)‑κB/p65 and protein kinase B (AKT) were determined using a western blotting assay. The neurological deficit score and infarct area induced by pMCAO decreased in a dose‑dependent manner following myricetin treatment. Furthermore, myricetin reduced the expression levels of IL‑1β, IL‑6, TNF‑α, and MDA, and increased GSH/GSSG ratio and SOD activity. A significant decrease in cell apoptosis was observed in response to myricetin. In addition, myricetin significantly increased the level of phosphorylated AKT protein, and decreased the phosphorylation of p38 MAPK and the level of NF‑κB/p65. Overall, the results of the present study suggested that myricetin exhibits a therapeutic effect by reducing ischemic cerebral injury, and the protective effect of myricetin may be associated with the p38 MAPK, NF‑κB/p65 and AKT signaling pathways.
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1 

Brill AK, Rösti R, Hefti JP, Bassetti C, Gugger M and Ott SR: Adaptive servo-ventilation as treatment of persistent central sleep apnea in post-acute ischemic stroke patients. Sleep Med. 15:1309–1313. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Jovanovic A, Stolic RV, Rasic DV, Markovic-Jovanovic SR and Peric VM: Stroke and diabetic ketoacidosis-some diagnostic and therapeutic considerations. Vasc Health Risk Manag. 10:201–204. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, Abraham J, Adair T, Aggarwal R, Ahn SY, et al: Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: A systematic analysis for the Global Burden of Disease Study 2010. Lancet. 380:2095–2128. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Murray CJ, Vos T, Lozano R, Naghavi M, Flaxman AD, Michaud C, Ezzati M, Shibuya K, Salomon JA, Abdalla S, et al: Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990–2010: A systematic analysis for the Global Burden of Disease Study 2010. Lancet. 380:2197–2223. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Writing Group Members, ; Mozaffarian D, Benjamin EJ, Go AS, Arnett DK, Blaha MJ, Cushman M, Das SR, de Ferranti S, Després JP, et al: Executive summary: Heart disease and stroke statistics-2016 update: A report from the American Heart Association. Circulation. 133:447–454. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Strong K, Mathers C and Bonita R: Preventing stroke: Saving lives around the world. Lancet Neurol. 6:182–187. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Tewari A, Mahendru V, Sinha A and Bilotta F: Antioxidants: The new frontier for translational research in cerebroprotection. J Anaesthesiol Clin Pharmacol. 30:160–171. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Liang LJ, Yang JM and Jin XC: Cocktail treatment, a promising strategy to treat acute cerebral ischemic stroke. Med Gas Res. 6:33–38. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Wang W, Ma X, Han J, Zhou M, Ren H, Pan Q, Zheng C and Zheng Q: Neuroprotective effect of scutellarin on ischemic cerebral injury by down-regulating the expression of angiotensin-converting enzyme and AT1 receptor. PLoS One. 11:e01461972016. View Article : Google Scholar : PubMed/NCBI

10 

Kang KA, Wang ZH, Zhang R, Piao MJ, Kim KC, Kang SS, Kim YW, Lee J, Park D and Hyun JW: Myricetin protects cells against oxidative stress-induced apoptosis via regulation of PI3K/Akt and MAPK signaling pathways. Int J Mol Sci. 11:4348–4360. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Majid M, Khan MR, Shah NA, UI Haq I, Farooq MA, Ullah S, Sharif A, Zahra Z, Younis T and Sajid M: Studies on phytochemical, antioxidant, anti-inflammatory and analgesic activities of Euphorbia dracunculoides. BMC Complement Altern Med. 15:3492015. View Article : Google Scholar : PubMed/NCBI

12 

Mendes V, Vilaça R, de Freitas V, Ferreira PM, Mateus N and Costa V: Effect of myricetin, pyrogallol, and phloroglucinol on yeast resistance to oxidative stress. Oxid Med Cell Longev. 2015:7825042015. View Article : Google Scholar : PubMed/NCBI

13 

Qiu Y, Cong N, Liang M, Wang Y and Wang J: Systems pharmacology dissection of the protective effect of myricetin against acute ischemia/reperfusion-induced myocardial injury in isolated rat heart. Cardiovasc Toxicol. 17:277–286. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Wu S, Yue Y, Peng A, Zhang L, Xiang J, Cao X, Ding H and Yin S: Myricetin ameliorates brain injury and neurological deficits via Nrf2 activation after experimental stroke in middle-aged rats. Food Funct. 7:2624–2634. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Longa EZ, Weinstein PR, Carlson S and Cummins R: Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 20:84–91. 1989. View Article : Google Scholar : PubMed/NCBI

16 

Livnat A, Barbiro-Michaely E and Mayevsky A: Mitochondrial function and cerebral blood flow variable responses to middle cerebral artery occlusion. J Neurosci Methods. 188:76–82. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Ahn HC, Yoo KY, Hwang IK, Cho JH, Lee CH, Choi JH, Li H, Cho BR, Kim YM and Won MH: Ischemia-related changes in naive and mutant forms of ubiquitin and neuroprotective effects of ubiquitin in the hippocampus following experimental transient ischemic damage. Exp Neurol. 220:120–132. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Xiang Y, Zhao H, Wang J, Zhang L, Liu A and Chen Y: Inflammatory mechanisms involved in brain injury following cardiac arrest and cardiopulmonary resuscitation. Biomed Rep. 5:11–17. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Chen YF, Wang YW, Huang WS, Lee MM, Wood WG, Leung YM and Tsai HY: Trans-Cinnamaldehyde, An essential oil in cinnamon powder, ameliorates cerebral ischemia-induced brain injury via inhibition of neuroinflammation through attenuation of iNOS, COX-2 expression and NFκ-B signaling pathway. Neuromolecular Med. 18:322–333. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Wang YS, Li YX, Zhao P, Wang HB, Zhou R, Hao YJ, Wang J, Wang SJ, Du J, Ma L, et al: Anti-inflammation effects of oxysophoridine on cerebral ischemia-reperfusion injury in mice. Inflammation. 38:2259–2268. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Xu S, Zhong A, Ma H, Li D, Hu Y, Xu Y and Zhang J: Neuroprotective effect of salvianolic acid B against cerebral ischemic injury in rats via the CD40/NF-κB pathway associated with suppression of platelets activation and neuroinflammation. Brain Res. 1661:37–48. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Chanput W, Krueyos N and Ritthiruangdej P: Anti-oxidative assays as markers for anti-inflammatory activity of flavonoids. Int Immunopharmacol. 40:170–175. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Chen YF, Wu KJ, Huang WS, Hsieh YW, Wang YW, Tsai HY and Lee MM: Neuroprotection of Gueichih-Fuling-Wan on cerebral ischemia/reperfusion injury in streptozotocin-induced hyperglycemic rats via the inhibition of the cellular apoptosis pathway and neuroinflammation. Biomedicine (Taipei). 6:212016. View Article : Google Scholar : PubMed/NCBI

24 

Zhang S, Shao SY, Song XY, Xia CY, Yang YN, Zhang PC and Chen NH: Protective effects of Forsythia suspense extract with antioxidant and anti-inflammatory properties in a model of rotenone induced neurotoxicity. Neurotoxicology. 52:72–83. 2016. View Article : Google Scholar : PubMed/NCBI

25 

Zhu L, Bi W and Lu D, Zhang C, Shu X and Lu D: Luteolin inhibits SH-SY5Y cell apoptosis through suppression of the nuclear transcription factor-κB, mitogen-activated protein kinase and protein kinase B pathways in lipopolysaccharide-stimulated cocultured BV2 cells. Exp Ther Med. 7:1065–1070. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Fu P, Wu Q, Hu J, Li T and Gao F: Baclofen protects primary rat retinal ganglion cells from chemical hypoxia-induced apoptosis through the Akt and PERK pathways. Front Cell Neurosci. 10:2552016. View Article : Google Scholar : PubMed/NCBI

27 

Ying Y, Zhu H, Liang Z, Ma X and Li S: GLP1 protects cardiomyocytes from palmitate-induced apoptosis via Akt/GSK3b/b-catenin pathway. J Mol Endocrinol. 55:245–262. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Qi Z, Qi S, Gui L, Shen L and Feng Z: Daphnetin protects oxidative stress-induced neuronal apoptosis via regulation of MAPK signaling and HSP70 expression. Oncol Lett. 12:1959–1964. 2016.PubMed/NCBI

29 

Suchal K, Malik S, Gamad N, Malhotra RK, Goyal SN, Chaudhary U, Bhatia J, Ojha S and Arya DS: Kaempferol attenuates myocardial ischemic injury via inhibition of MAPK signaling pathway in experimental model of myocardial ischemia-reperfusion injury. Oxid Med Cell Longev. 2016:75807312016. View Article : Google Scholar : PubMed/NCBI

30 

Apostolatos A, Song S, Acosta S, Peart M, Watson JE, Bickford P, Cooper DR and Patel NA: Insulin promotes neuronal survival via the alternatively spliced protein kinase CδII isoform. J Biol Chem. 287:9299–9310. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Cheng CY, Lin JG, Tang NY, Kao ST and Hsieh CL: Electroacupuncture at different frequencies (5Hz and 25Hz) ameliorates cerebral ischemia-reperfusion injury in rats: Possible involvement of p38 MAPK-mediated anti-apoptotic signaling pathways. BMC Complement Altern Med. 15:2412015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun L, Xu P, Fu T, Huang X, Song J, Chen M, Tian X, Yin H and Han J: Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model. Mol Med Rep 17: 3274-3280, 2018.
APA
Sun, L., Xu, P., Fu, T., Huang, X., Song, J., Chen, M. ... Han, J. (2018). Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model. Molecular Medicine Reports, 17, 3274-3280. https://doi.org/10.3892/mmr.2017.8212
MLA
Sun, L., Xu, P., Fu, T., Huang, X., Song, J., Chen, M., Tian, X., Yin, H., Han, J."Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model". Molecular Medicine Reports 17.2 (2018): 3274-3280.
Chicago
Sun, L., Xu, P., Fu, T., Huang, X., Song, J., Chen, M., Tian, X., Yin, H., Han, J."Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model". Molecular Medicine Reports 17, no. 2 (2018): 3274-3280. https://doi.org/10.3892/mmr.2017.8212
Copy and paste a formatted citation
x
Spandidos Publications style
Sun L, Xu P, Fu T, Huang X, Song J, Chen M, Tian X, Yin H and Han J: Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model. Mol Med Rep 17: 3274-3280, 2018.
APA
Sun, L., Xu, P., Fu, T., Huang, X., Song, J., Chen, M. ... Han, J. (2018). Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model. Molecular Medicine Reports, 17, 3274-3280. https://doi.org/10.3892/mmr.2017.8212
MLA
Sun, L., Xu, P., Fu, T., Huang, X., Song, J., Chen, M., Tian, X., Yin, H., Han, J."Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model". Molecular Medicine Reports 17.2 (2018): 3274-3280.
Chicago
Sun, L., Xu, P., Fu, T., Huang, X., Song, J., Chen, M., Tian, X., Yin, H., Han, J."Myricetin against ischemic cerebral injury in rat middle cerebral artery occlusion model". Molecular Medicine Reports 17, no. 2 (2018): 3274-3280. https://doi.org/10.3892/mmr.2017.8212
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