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Article

Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan

  • Authors:
    • Yuping Yin
    • Qian Shen
    • Ruikang Tao
    • Weilong Chang
    • Ruidong Li
    • Gengchen Xie
    • Weizhen Liu
    • Peng Zhang
    • Kaixiong Tao
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Oncology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Center for Biomolecular Science and Engineering, University of California Santa Cruz, Santa Cruz, CA 95064, USA, Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou, Zhengzhou, Henan 450000, P.R. China
  • Pages: 3344-3349
    |
    Published online on: December 8, 2017
       https://doi.org/10.3892/mmr.2017.8230
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Abstract

Wee1 is an oncogenic nuclear kinase, which can regulate the cell cycle as a crucial G2M checkpoint. Overexpression of Wee1 can be observed in various cancer types, which may lead to a poor prognosis, but the potential therapeutic value of Wee1 in colorectal cancer has not been fully studied. In the present study, the role of Wee1 in colonic cancer was investigated. Wee1 inhibition by small interfering RNA was demonstrated to significantly restrain cancer cell proliferation and sensitize the p53 mutant colonic cancer cell lines HT29 and SW480 to the effect of treatment with ionizing radiation. The anticancer effect of the Wee1 inhibitor MK1775 was investigated in these two colonic cancer cell lines. MK1775 was demonstrated to induce significant DNA damage, suppress cell viability and induce apoptosis. In addition, MK1775 sensitized HT29 and SW480 cells to the effect of irinotecan. Annexin V/propidium iodide staining demonstrated that combination therapy can induce increased apoptosis compared with MK1775 or irinotecan monotherapy. The results of western blot analysis also indicated increased expression of the DNA damage marker histone H2AX, and apoptosis‑associated protein cleaved caspase 3, in HT29 and SW480 cells. In conclusion, the present study indicated that Wee1 may be a valuable target for treatment of p53 mutant colonic cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Yin Y, Shen Q, Tao R, Chang W, Li R, Xie G, Liu W, Zhang P and Tao K: Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan. Mol Med Rep 17: 3344-3349, 2018.
APA
Yin, Y., Shen, Q., Tao, R., Chang, W., Li, R., Xie, G. ... Tao, K. (2018). Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan. Molecular Medicine Reports, 17, 3344-3349. https://doi.org/10.3892/mmr.2017.8230
MLA
Yin, Y., Shen, Q., Tao, R., Chang, W., Li, R., Xie, G., Liu, W., Zhang, P., Tao, K."Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan". Molecular Medicine Reports 17.2 (2018): 3344-3349.
Chicago
Yin, Y., Shen, Q., Tao, R., Chang, W., Li, R., Xie, G., Liu, W., Zhang, P., Tao, K."Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan". Molecular Medicine Reports 17, no. 2 (2018): 3344-3349. https://doi.org/10.3892/mmr.2017.8230
Copy and paste a formatted citation
x
Spandidos Publications style
Yin Y, Shen Q, Tao R, Chang W, Li R, Xie G, Liu W, Zhang P and Tao K: Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan. Mol Med Rep 17: 3344-3349, 2018.
APA
Yin, Y., Shen, Q., Tao, R., Chang, W., Li, R., Xie, G. ... Tao, K. (2018). Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan. Molecular Medicine Reports, 17, 3344-3349. https://doi.org/10.3892/mmr.2017.8230
MLA
Yin, Y., Shen, Q., Tao, R., Chang, W., Li, R., Xie, G., Liu, W., Zhang, P., Tao, K."Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan". Molecular Medicine Reports 17.2 (2018): 3344-3349.
Chicago
Yin, Y., Shen, Q., Tao, R., Chang, W., Li, R., Xie, G., Liu, W., Zhang, P., Tao, K."Wee1 inhibition can suppress tumor proliferation and sensitize p53 mutant colonic cancer cells to the anticancer effect of irinotecan". Molecular Medicine Reports 17, no. 2 (2018): 3344-3349. https://doi.org/10.3892/mmr.2017.8230
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