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A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats

  • Authors:
    • Hong Yin
    • Tao Jiang
    • Xi Deng
    • Miao Yu
    • Hui Xing
    • Xianjun Ren
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China, Department of Ultrasound, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, P.R. China
    Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2998-3004
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    Published online on: December 11, 2017
       https://doi.org/10.3892/mmr.2017.8238
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Abstract

Spinal cord injury (SCI), usually resulting in severe sensory and motor deficits, is a major public health concern. Adipose‑derived stem cells (ADSCs), one type of adult stem cell, are free from ethical restriction, easily isolated and enriched. Therefore, ADSCs may provide a feasible cell source for cell‑based therapies in treatment of SCI. The present study successfully isolated rat ADSCs (rADSCs) from Sprague‑Dawley male rats and co‑cultured them with acellular spinal cord scaffolds (ASCs). Then, a rat spinal cord hemisection model was built and rats were randomly divided into 3 groups: SCI only, ASC only, and ASC + ADSCs. Furthermore, behavioral tests were conducted to evaluate functional recovery. Hematoxylin & Eosin staining and immunofluorence were carried out to assess histopathological remodeling. In addition, biotinylated dextran amines anterograde tracing was employed to visualize axon regeneration. The data demonstrated that harvested cells, which were positive for cell surface antigen cluster of differentiation (CD) 29, CD44 and CD90 and negative for CD4, detected by flow cytometry analysis, held the potential to differentiate into osteocytes and adipocytes. Rats that received transplantation of ASCs seeded with rADSCs benefited greatly in functional recovery through facilitation of histopathological rehabilitation, axon regeneration and reduction of reactive gliosis. rADSCs co‑cultured with ASCs may survive and integrate into the host spinal cord on day 14 post‑SCI.
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Copy and paste a formatted citation
Spandidos Publications style
Yin H, Jiang T, Deng X, Yu M, Xing H and Ren X: A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats. Mol Med Rep 17: 2998-3004, 2018.
APA
Yin, H., Jiang, T., Deng, X., Yu, M., Xing, H., & Ren, X. (2018). A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats. Molecular Medicine Reports, 17, 2998-3004. https://doi.org/10.3892/mmr.2017.8238
MLA
Yin, H., Jiang, T., Deng, X., Yu, M., Xing, H., Ren, X."A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats". Molecular Medicine Reports 17.2 (2018): 2998-3004.
Chicago
Yin, H., Jiang, T., Deng, X., Yu, M., Xing, H., Ren, X."A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats". Molecular Medicine Reports 17, no. 2 (2018): 2998-3004. https://doi.org/10.3892/mmr.2017.8238
Copy and paste a formatted citation
x
Spandidos Publications style
Yin H, Jiang T, Deng X, Yu M, Xing H and Ren X: A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats. Mol Med Rep 17: 2998-3004, 2018.
APA
Yin, H., Jiang, T., Deng, X., Yu, M., Xing, H., & Ren, X. (2018). A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats. Molecular Medicine Reports, 17, 2998-3004. https://doi.org/10.3892/mmr.2017.8238
MLA
Yin, H., Jiang, T., Deng, X., Yu, M., Xing, H., Ren, X."A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats". Molecular Medicine Reports 17.2 (2018): 2998-3004.
Chicago
Yin, H., Jiang, T., Deng, X., Yu, M., Xing, H., Ren, X."A cellular spinal cord scaffold seeded with rat adipose‑derived stem cells facilitates functional recovery via enhancing axon regeneration in spinal cord injured rats". Molecular Medicine Reports 17, no. 2 (2018): 2998-3004. https://doi.org/10.3892/mmr.2017.8238
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