Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
February-2018 Volume 17 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2018 Volume 17 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway

  • Authors:
    • Huanhuan Chen
    • Yuechun Huang
    • Dandan Huang
    • Zhifang Wu
    • Yunrong Li
    • Chunhua Zhou
    • Gang Wei
  • View Affiliations / Copyright

    Affiliations: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Department of Pharmacy, The First Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510405, P.R. China, Department of Trauma Orthopedics, The Third Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510360, P.R. China
  • Pages: 3267-3273
    |
    Published online on: December 11, 2017
       https://doi.org/10.3892/mmr.2017.8242
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Bone marrow mesenchymal stem cell (BMSC) transplants are promising for the treatment of certain central nervous system diseases. However, oxidative stress is one of the major factors that may limit the survival of the transplanted BMSCs. The present study investigated the effect of pretreatment with gigantol on hydrogen peroxide (H2O2)‑induced apoptosis in rat BMSCs (rBMSCs) and the potential underlying mechanisms. The results demonstrated that gigantol pretreatment significantly inhibited H2O2‑induced apoptosis of rBMSCs. rBMSCs were incubated with 600 µM H2O2 in the absence or presence of different doses of gigantol (1‑100 µM). Cell viability and apoptosis ratios were assessed by MTT assays and flow cytometry, respectively. Morphological alterations and reactive oxygen species were measured by the fluorescent‑based methods of Hoechst staining and dichlorodihydrofluorescein diacetate, respectively. Furthermore, the protein levels of phosphorylated‑protein kinase B (Akt), B‑cell lymphoma‑2 (Bcl‑2), Bcl‑2‑associated X (Bax), caspase‑3 and caspase‑9 were investigated by western blotting. Following incubation with H2O2 for 2 h, gigantol significantly inhibited the H2O2‑induced reductions in the cell viability of rBMSCs in a dose‑dependent manner. Furthermore, gigantol upregulated Akt phosphorylation and Bcl‑2 expression, downregulated Bax expression, and reduced the expression of caspase‑3 and caspase‑9 in H2O2‑treated rBMSCs, whereas an opposite effect was detected when LY294002, an inhibitor of phosphatidylinositol 3‑kinase, was administered in combination with gigantol. These results indicate that gigantol may be developed as a promising neuroprotective agent for successful MSC transplantation in ischemic diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Tuan RS, Boland G and Tuli R: Adult mesenchymal stem cells and cell-based tissue engineering. Arthritis Res Ther. 5:32–45. 2003. View Article : Google Scholar : PubMed/NCBI

2 

De Keyser J: Autologous mesenchymal stem cell transplantation in stroke patients. Ann Neurol. 58:653–654. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Monsel A, Zhu YG, Gennai S, Hao Q, Liu J and Lee JW: Cell-based therapy for acute organ injury: Preclinical evidence and ongoing clinical trials using mesenchymal stem cells. Anesthesiology. 121:1099–1121. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Hao L, Zou Z, Tian H, Zhang Y, Zhou H and Liu L: Stem cell-based therapies for ischemic stroke. Biomed Res Int. 2014:4687482014. View Article : Google Scholar : PubMed/NCBI

5 

Lee JH, Jung HK, Han YS, Yoon YM, Yun CW, Sun HY, Cho HW and Lee SH: Antioxidant effects of Cirsium setidens extract on oxidative stress in human mesenchymal stem cells. Mol Med Rep. 14:3777–3784. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Wei H, Li Z, Hu S, Chen X and Cong X: Apoptosis of mesenchymal stem cells induced by hydrogen peroxide concerns both endoplasmic reticulum stress and mitochondrial death pathway through regulation of caspases, p38 and JNK. J Cell Biochem. 111:967–978. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Li Y, Wang ZT and Xu LS: Phenols and a triterpene from Dendrobium aurantiacum var. Denneanum (Orchidaceae). Biochem Syst Ecol. 34:658–660. 2006. View Article : Google Scholar

8 

Kuppusamy S, Thavamani P, Megharaj M, Nirola R, Lee YB and Naidu R: Assessment of antioxidant activity, minerals, phenols and flavonoid contents of common plant/tree waste extracts. Ind Crop Prod. 83:630–634. 2016. View Article : Google Scholar

9 

Wang ST, Gao W, Fan YX, Liu XG, Liu K, Du Y, Wang LL, Li HJ, Li P and Yang H: Phenol profiles and antioxidant capacities of bistort rhizoma (Polygonum bistorta L.) extracts. Rsc Adv. 6:27320–27328. 2016. View Article : Google Scholar

10 

Temel E, Alasalvar C, Gokce H, Güder A, Albayrak Ç, Alpaslan YB, Alpaslan G and Dilek N: DFT calculations, spectroscopy and antioxidant activity studies on (E)-2-nitro-4-((phenylimino)methyl)phenol. Spectrochim Acta A. 136:534–546. 2015. View Article : Google Scholar

11 

Menshchikova EB, Wisman NY, Zenkov NK, Tkachev VO and Kandalintseva NV: ARE-inducing phenol antioxidant TC-13 improves survival of drosophila melanogaster in oxidative stress. B Exp Med. 154:260–264. 2012. View Article : Google Scholar

12 

Fang H, Hu X, Wang M, Wan W, Yang Q, Sun X, Gu Q, Gao X, Wang Z, Gu L, et al: Anti-osmotic and antioxidant activities of gigantol from Dendrobium aurantiacum var. Denneanum against cataractogenesis in galactosemic rats. J Ethnopharmacol. 172:238–246. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Chen H, Huang Y, Huang J, Lin LZ and Wei G: Gigantol attenuates the proliferation of human liver cancer through the PI3K/Akt/NF-κB signaling pathway. Oncol Rep. 37:865–870. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Bhummaphan N and Chanvorachote P: Gigantol suppresses cancer stem cell-like phenotypes in lung cancer cells. Evid Based Complement Alternal Med. 2015:1–10. 2015. View Article : Google Scholar

15 

Miyazawa M, Shimamura H, Nakamura S and Kameoka H: Antimutagenic activity of gigantol from Dendrobium nobile. J Agrc Food Chem. 45:2849–2853. 1997. View Article : Google Scholar

16 

Won JH, Kim JY, Yun KJ, Lee JH, Back NI, Chung HG, Chung SA, Jeong TS, Choi MS and Lee KT: Gigantol isolated from the whole plants of cymbidium goeringii inhibits the LPS-induced iNOS and COX-2 expression via NF-κB inactivation in RAW 264.7 macrophages cells. Planta Med. 72:1181–1187. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Wu J, Lu C, Li X, Fang H, Wan W, Yang Q, Sun X, Wang M, Hu X, Chen CY and Wei X: Synthesis and biological evaluation of novel gigantol derivatives as potential agents in prevention of diabetic cataract. PLoS One. 10:e01410922015. View Article : Google Scholar : PubMed/NCBI

18 

Tropel P, Noël D, Platet N, Legrand P, Benabid AL and Berger F: Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow. Exp Cell Res. 295:395–406. 2004. View Article : Google Scholar : PubMed/NCBI

19 

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals: Guide for the Care and Use of Laboratory Animals. 8th. National Academies Press (US); Washington, DC: 2011

20 

Gutiérrez-Fernández M, Rodríguez-Frutos B, Ramos-Cejudo J, Teresa Vallejo-Cremades M, Fuentes B, Cerdán S and Díez-Tejedor E: Effects of intravenous administration of allogenic bone marrow- and adipose tissue-derived mesenchymal stem cells on functional recovery and brain repair markers in experimental ischemic stroke. Stem Cell Res. 4:112013. View Article : Google Scholar

21 

Wei L, Fraser JL, Lu ZY, Hu X and Yu SP: Transplantation of hypoxia preconditioned bone marrow mesenchymal stem cells enhances angiogenesis and neurogenesis after cerebral ischemia in rats. Neurobiol Dis. 46:635–645. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Tsai LK, Wang Z, Munasinghe J, Leng Y, Leeds P and Chuang DM: Mesenchymal stem cells primed with valproate and lithium robustly migrate to infarcted regions and facilitate recovery in a stroke model. Stroke. 42:2932–2939. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Lin YC, Huang YC, Chen SC, Liaw CC, Kuo SC, Huang LJ and Gean PW: Neuroprotective effects of ugonin K on hydrogen peroxide-induced cell death in human neuroblastoma SH-SY5Y cells. Neurochem Res. 34:923–930. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Zhang Q, Huang WD, Lv XY and Yang YM: Puerarin protects differentiated PC12 cells from H2O2-induced apoptosis through the PI3K/Akt signalling pathway. Cell Biol Int. 36:419–426. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Lee AY, Wu TT, Hwang BR, Lee J, Lee MH, Lee S and Cho EJ: The neuro-protective effect of the methanolic extract of Perilla frutescens var. Japonica and rosmarinic acid against H2O2-induced oxidative stress in C6 glial cells. Biomol Ther (Seoul). 24:338–345. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Sun B, Feng M, Tian X, Lu X, Zhang Y, Ke X, Huang S, Cao J and Ding X: Dl-3-n-Butylphthalide protects rat bone marrow stem cells against hydrogen peroxide-induced cell death through antioxidation and activation of PI3K-Akt pathway. Neurosci Lett. 516:247–252. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Tao SC, Chen ZH, Huang KW, Yan MX and Wei G; Guangzhou University of Chinese Medicine, ; Foshan Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Chinese Medicine, : Comparative study of HPLC characteristic spectrum of Dendrobium fimbriatum hook and other huangcao dendrobium species. Chin Med Phar. 27:238–241. 2016.

28 

Li Y, Xue F, Xu SZ, Wang XW, Tong X and Lin XJ: Lycopene protects bone marrow mesenchymal stem cells against ischemia-induced apoptosis in vitro. Eur Rev Med Pharmaco. 18:1625–1631. 2014.

29 

Stambolic V and Woodgett JR: Functional distinctions of protein kinase B/Akt isoforms defined by their influence on cell migration. Trends Cell Bio. 16:461–466. 2006. View Article : Google Scholar

30 

Osaki M, Oshimura M and Ito H: PI3KAkt pathway: Its functions and alterations in human cancer. Apoptosis. 9:667–676. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Song G, Ouyang G and Bao S: The activation of Akt/PIK13 signaling pathway and cell survival. J Cell Mol Med. 9:59–71. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Downward J: PI 3-kinase, Akt and cell survival. Semin Cell Dev Biol. 15:177–182. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Kim JY, Lee JS, Han YS, Lee JH, Bae I, Yoon YM, Kwon SM and Lee SH: Pretreatment with lycopene attenuates oxidative stress-induced apoptosis in human mesenchymal stem cells. Biomol Ther. 23:517–524. 2015. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen H, Huang Y, Huang D, Wu Z, Li Y, Zhou C and Wei G: Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway. Mol Med Rep 17: 3267-3273, 2018.
APA
Chen, H., Huang, Y., Huang, D., Wu, Z., Li, Y., Zhou, C., & Wei, G. (2018). Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway. Molecular Medicine Reports, 17, 3267-3273. https://doi.org/10.3892/mmr.2017.8242
MLA
Chen, H., Huang, Y., Huang, D., Wu, Z., Li, Y., Zhou, C., Wei, G."Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway". Molecular Medicine Reports 17.2 (2018): 3267-3273.
Chicago
Chen, H., Huang, Y., Huang, D., Wu, Z., Li, Y., Zhou, C., Wei, G."Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway". Molecular Medicine Reports 17, no. 2 (2018): 3267-3273. https://doi.org/10.3892/mmr.2017.8242
Copy and paste a formatted citation
x
Spandidos Publications style
Chen H, Huang Y, Huang D, Wu Z, Li Y, Zhou C and Wei G: Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway. Mol Med Rep 17: 3267-3273, 2018.
APA
Chen, H., Huang, Y., Huang, D., Wu, Z., Li, Y., Zhou, C., & Wei, G. (2018). Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway. Molecular Medicine Reports, 17, 3267-3273. https://doi.org/10.3892/mmr.2017.8242
MLA
Chen, H., Huang, Y., Huang, D., Wu, Z., Li, Y., Zhou, C., Wei, G."Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway". Molecular Medicine Reports 17.2 (2018): 3267-3273.
Chicago
Chen, H., Huang, Y., Huang, D., Wu, Z., Li, Y., Zhou, C., Wei, G."Protective effect of gigantol against hydrogen peroxide‑induced apoptosis in rat bone marrow mesenchymal stem cells through the PI3K/Akt pathway". Molecular Medicine Reports 17, no. 2 (2018): 3267-3273. https://doi.org/10.3892/mmr.2017.8242
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team