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Article

Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B

  • Authors:
    • Xiaoxia Wu
    • Kaiming Li
    • Meihua Xie
    • Minjun Yu
    • Shuangyang Tang
    • Zhenyu Li
    • Sihai Hu
  • View Affiliations / Copyright

    Affiliations: Laboratory of Anti‑infectious Immunity, Pathogenic Biology Institute, College of Basic Medicine, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 3178-3185
    |
    Published online on: December 12, 2017
       https://doi.org/10.3892/mmr.2017.8255
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Abstract

Neisseria meningitidis (N. meningitidis) is a major cause of meningitis and sepsis. Capsular polysaccharide‑based vaccines against serogroups A, C, Y, and W135 are available; however, the development of a vaccine against N. meningitidis serogroup B (NMB) has been problematic. NMB0315 is an outer membrane protein of NMB that may be a virulence factor for N. meningitidis and a possible target for functional bactericidal antibodies. The present study aimed to develop a potent DNA vaccine against NMB by cloning the NMB0135 gene into the pcDNA3.1(+) vector to construct the recombinant plasmid pcDNA3.1(+)/NMB0315 (designated pNMB0315). pNMB0315 was transfected into eukaryotic COS‑7 and RAW264.7 cells to express the recombinant (r)NMB0315 protein. Protective immunogenicity of the DNA vaccine was assessed in an in vivo mouse model. The levels of rNMB0315‑specific immunoglobulin G (IgG), IgG1 and IgG2a antibodies in the pNMB0315‑immunized group increased dramatically up to week 6 following the initial vaccination, and were significantly higher compared with the levels in the Control groups. The serum concentrations of interleukin‑4 and interferon‑γ were significantly higher in the pNMB0315‑immunized group compared with the control groups. Following intraperitoneal challenge with a lethal dose of NMB strain MC58, the survival rate in the pNMB0315 + CpG group was 70% (14 out of 20 mice) at 14 days; by contrast, all mice in the control groups succumbed within 3 days. The serum bactericidal titers of the pNMB0315 + CpG group in vitro reached 1:128 following three immunizations. The results indicated that pNMB0315 may serve as a promising DNA vaccine against NMB.
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1 

Shahbaaz M, Bisetty K, Ahmad F and Hassan MI: Towards new drug targets? function prediction of putative proteins of Neisseria meningitidis MC58 and their virulence characterization. OMICS. 19:416–434. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Silva GP, Cruz SC, Cruz AC and Milagres LG: Short-term and long-term antibody response by mice after immunization against Neisseria meningitidis B or diphtheria toxoid. Braz J Med Biol Res. 46:148–153. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Ying S, He J, Yu M, Zhang Y, Deng S, Zhang L, Xie M and Hu S: Recombinant Neisseria surface protein A is a potential vaccine candidate against Neisseria meningitides serogroup B. Mol Med Rep. 10:1619–1625. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Pelton SI: The global evolution of meningococcal epidemiology following the introduction of meningococcal vaccines. J Adolesc Health. 59 Suppl 2:S3–S11. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Braunstein M, Rajkumar P, Claus CL, Vaccarelli G, Moore AJ, Wang D and Anderson MK: HEBAlt enhances the T-cell potential of fetal myeloid-biased precursors. Int Immunol. 22:963–972. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Brehony C, Rodrigues CM, Borrow R, Smith A, Cunney R, Moxon ER and Maiden MCJ: Distribution of bexsero® antigen sequence types (BASTs) in invasive meningococcal disease isolates: Implications for immunisation. Vaccine. 34:4690–4697. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Sung JW, Hsieh SY, Lin CL, Leng CH, Liu SJ, Chou AH, Lai LW, Lin LH, Kwok Y, Yang CY and Chong P: Biochemical characterizations of Escherichia coli-expressed protective antigen Ag473 of Neisseria meningitides group B. Vaccine. 28:8175–8182. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Khatami A and Pollard AJ: The epidemiology of meningococcal disease and the impact of vaccines. Expert Rev Vaccines. 9:285–298. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Martin D, Brodeur BR, Hamel J, Couture F, de Alwis U, Lian Z, Martin S, Andrews D and Ellis RW: Candidate Neisseria meningitidis NspA vaccine. J Biotechnol. 83:27–31. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Wang X, Yang X, Yang C, Wu Z, Xu H and Shen Y: Crystal structure of outer membrane protein NMB0315 from Neisseria meningitidis. PLoS One. 6:e268452011. View Article : Google Scholar : PubMed/NCBI

11 

Lee J, Kang HE and Woo HJ: Protective immunity conferred by the C-terminal fragment of recombinant Pasteurella multocida toxin. Clin Vaccine Immunol. 19:1526–1531. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Fang Y, Lin H, Ma Z and Fan H: Construction and immunogenicity of recombinant swinepox virus expressing outer membrane protein L of salmonella. J Microbiol Biotechnol. 26:1173–1181. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Borrow R, Carlone GM, Rosenstein N, Blake M, Feavers I, Martin D, Zollinger W, Robbins J, Aaberge I, Granoff DM, et al: Neisseria meningitidis group B correlates of protection and assay standardization-International Meeting Report Emory University, Atlanta, Georgia, United States, 16–17 March 2005. Vaccine. 24:5093–5107. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Johansson L, Rytkonen A, Bergman P, Albiger B, Källström H, Hökfelt T, Agerberth B, Cattaneo R and Jonsson AB: CD46 in meningococcal disease. Science. 301:373–375. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Sjölinder H and Jonsson AB: Olfactory nerve-a novel invasion route of Neisseria meningitidis to reach the meninges. PLoS One. 5:e140342010. View Article : Google Scholar : PubMed/NCBI

16 

Chaudhari A, Pathakota GB and Annam PK: Design and construction of shrimp antiviral DNA vaccines expressing long and short hairpins for protection by RNA interference. Methods Mol Biol. 1404:225–240. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Abdulhaqq SA and Weiner DB: DNA vaccines: Developing new strategies to enhance immune responses. Immunol Res. 42:219–232. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Wu QQ, Zhang QH and Ma L: Construction of recombinant eukaryotic expression plasmid pcDNA3.1(+)-mtDNA of human colorectal carcinoma cells. Di Yi Jun Yi Da Xue Xue Bao. 25:1016–1019. 2005.(In Chinese). PubMed/NCBI

19 

Cheng WK, Plumb AW, Lai JC, Abraham N and Dutz JP: Topical CpG oligodeoxynucleotide adjuvant enhances the adaptive immune response against influenza A infections. Front Immunol. 7:2842016. View Article : Google Scholar : PubMed/NCBI

20 

Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K and Akira S: A Toll-like receptor recognizes bacterial DNA. Nature. 408:740–745. 2000. View Article : Google Scholar : PubMed/NCBI

21 

Wu HM, Wang J, Zhang B, Fang L, Xu K and Liu RY: CpG-ODN promotes phagocytosis and autophagy through JNK/P38 signal pathway in Staphylococcus aureus-stimulated macrophage. Life Sci. 161:51–59. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Buchanan RM, Briles DE, Arulanandam BP, Westerink MA, Raeder RH and Metzger DW: IL-12-mediated increases in protection elicited by pneumococcal and meningococcal conjugate vaccines. Vaccine. 19:2020–2028. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Gong W, Qi Y, Xiong X, Jiao J, Duan C and Wen B: Rickettsia rickettsii outer membrane protein YbgF induces protective immunity in C3H/HeN mice. Hum Vaccin Immunother. 11:642–649. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Trotter CL, Yaro S, Njanpop-Lafourcade BM, Drabo A, Kroman SS, Idohou RS, Sanou O, Bowen L, Findlow H, Diagbouga S, et al: Seroprevalence of bactericidal, specific IgG antibodies and incidence of meningitis due to group A Neisseria meningitidis by age in Burkina Faso 2008. PLoS One. 8:e554862013. View Article : Google Scholar : PubMed/NCBI

25 

Bash MC, Lynn F, Mocca B, Borrow R, Findlow H, Hassan-King M, Preziosi MP, Idoko O, Sow S, Kulkarni P and Laforce FM: Development and use of a serum bactericidal assay using pooled human complement to assess responses to a meningococcal group A conjugate vaccine in African toddlers. Clin Vaccine Immunol. 21:755–761. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Findlow J, Holland A, Andrews N, Weynants V, Sotolongo F, Balmer P, Poolman J and Borrow R: Comparison of phenotypically indistinguishable but geographically distinct Neisseria meningitidis group B isolates in a serum bactericidal antibody assay. Clin Vaccine Immunol. 14:1451–1477. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu X, Li K, Xie M, Yu M, Tang S, Li Z and Hu S: Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B. Mol Med Rep 17: 3178-3185, 2018.
APA
Wu, X., Li, K., Xie, M., Yu, M., Tang, S., Li, Z., & Hu, S. (2018). Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B. Molecular Medicine Reports, 17, 3178-3185. https://doi.org/10.3892/mmr.2017.8255
MLA
Wu, X., Li, K., Xie, M., Yu, M., Tang, S., Li, Z., Hu, S."Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B". Molecular Medicine Reports 17.2 (2018): 3178-3185.
Chicago
Wu, X., Li, K., Xie, M., Yu, M., Tang, S., Li, Z., Hu, S."Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B". Molecular Medicine Reports 17, no. 2 (2018): 3178-3185. https://doi.org/10.3892/mmr.2017.8255
Copy and paste a formatted citation
x
Spandidos Publications style
Wu X, Li K, Xie M, Yu M, Tang S, Li Z and Hu S: Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B. Mol Med Rep 17: 3178-3185, 2018.
APA
Wu, X., Li, K., Xie, M., Yu, M., Tang, S., Li, Z., & Hu, S. (2018). Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B. Molecular Medicine Reports, 17, 3178-3185. https://doi.org/10.3892/mmr.2017.8255
MLA
Wu, X., Li, K., Xie, M., Yu, M., Tang, S., Li, Z., Hu, S."Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B". Molecular Medicine Reports 17.2 (2018): 3178-3185.
Chicago
Wu, X., Li, K., Xie, M., Yu, M., Tang, S., Li, Z., Hu, S."Construction and protective immunogenicity of DNA vaccine pNMB0315 against Neisseria meningitidis serogroup B". Molecular Medicine Reports 17, no. 2 (2018): 3178-3185. https://doi.org/10.3892/mmr.2017.8255
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