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Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway

  • Authors:
    • Chun‑Xia Wan
    • Man Xu
    • Si‑Hui Huang
    • Qing‑Qing Wu
    • Yuan Yuan
    • Wei Deng
    • Qi‑Zhu Tang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Wan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3085-3091
    |
    Published online on: December 12, 2017
       https://doi.org/10.3892/mmr.2017.8266
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Abstract

The involvement of vascular endothelial injury with the pathophysiological process of heart failure has been identified. Baicalein (BAI), a flavonoid extracted from the root of Scutellaria baicalensis, is reported to exert antibacterial, antiviral, antithrombotic and antioxidant effects. The aim of the present study was to investigate the effects of BAI on lipopolysaccharide (LPS)‑induced vascular endothelial injury. Human umbilical vein endothelial cells (HUVECs) were stimulated by LPS (10 µM) in the presence or absence of BAI. The expressions of the inflammatory cytokines interleukin (IL)‑lβ, IL‑6, tumor necrosis factor‑α (TNF‑α) and monocyte chemoattractant protein‑1 (MCP‑1) were analyzed by reverse transcription‑quantitative polymerase chain reaction, western blotting and enzyme‑linked immunosorbent assay. Cell apoptosis was assessed by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The results showed that BAI significantly inhibited the LPS‑induced inflammatory response and apoptosis in HUVECs. BAI suppressed the LPS‑induced upregulation of IL‑1β, IL‑6, TNF‑α and MCP‑1. Furthermore, BAI decreased the expression of B‑cell lymphoma 2 (Bcl‑2)‑associated X protein and cleaved caspase‑3; however, it increased the protein level of Bcl‑2. The inhibitory effect of BAI may occur through the suppression of the Toll‑like receptor 4 (TLR4)/phosphorylated (p)‑transforming growth factor β‑activated kinase 1/tumor necrosis factor receptor‑associated family member associated nuclear factor (NF)‑κB activator‑binding kinase 1 (p‑TBK1)/NF‑κB signaling pathway. An increase in the level of p‑TBK1 by MRT67307 abolished the effect of BAI on p‑p65. In conclusion, the results of the present research suggested that BAI ameliorated endothelial cell injury associated with TLR4/NF‑κB signaling, and highlighted the potential clinical use of BAI in blocking endothelial dysfunction and preventing heart failure.
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Copy and paste a formatted citation
Spandidos Publications style
Wan CX, Xu M, Huang SH, Wu QQ, Yuan Y, Deng W and Tang QZ: Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway. Mol Med Rep 17: 3085-3091, 2018.
APA
Wan, C., Xu, M., Huang, S., Wu, Q., Yuan, Y., Deng, W., & Tang, Q. (2018). Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway. Molecular Medicine Reports, 17, 3085-3091. https://doi.org/10.3892/mmr.2017.8266
MLA
Wan, C., Xu, M., Huang, S., Wu, Q., Yuan, Y., Deng, W., Tang, Q."Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway". Molecular Medicine Reports 17.2 (2018): 3085-3091.
Chicago
Wan, C., Xu, M., Huang, S., Wu, Q., Yuan, Y., Deng, W., Tang, Q."Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway". Molecular Medicine Reports 17, no. 2 (2018): 3085-3091. https://doi.org/10.3892/mmr.2017.8266
Copy and paste a formatted citation
x
Spandidos Publications style
Wan CX, Xu M, Huang SH, Wu QQ, Yuan Y, Deng W and Tang QZ: Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway. Mol Med Rep 17: 3085-3091, 2018.
APA
Wan, C., Xu, M., Huang, S., Wu, Q., Yuan, Y., Deng, W., & Tang, Q. (2018). Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway. Molecular Medicine Reports, 17, 3085-3091. https://doi.org/10.3892/mmr.2017.8266
MLA
Wan, C., Xu, M., Huang, S., Wu, Q., Yuan, Y., Deng, W., Tang, Q."Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway". Molecular Medicine Reports 17.2 (2018): 3085-3091.
Chicago
Wan, C., Xu, M., Huang, S., Wu, Q., Yuan, Y., Deng, W., Tang, Q."Baicalein protects against endothelial cell injury by inhibiting the TLR4/NF‑κB signaling pathway". Molecular Medicine Reports 17, no. 2 (2018): 3085-3091. https://doi.org/10.3892/mmr.2017.8266
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